Molecular Cloning and Characterization of a Novel Protein Kinase C-Interacting Protein with Structural Motifs Related to RBCC Family Proteins

A family of structurally related RING finger proteins interacts specificallywith the ubiquitin‐conjugating enzyme UbcM41

“…UIP28 is the mouse homologue of the previously described protein RBCK1 from rat. This protein was identified by the yeast two-hybrid screen as a protein kinase C (PKC)-interacting protein and it has been speculated that it might be a transcription factor that has a role in the signaling pathway through PKC [16,28]. The genes encoding UIP77 and UIP48 are the mouse homologues of the Drosophila genes Ariadne-1 and Ariadne-2, respectively.…”

“…Comparison of amino acid sequences predicted from the open reading frames of the U I P clones with protein sequences of the G e n B a n k data base revealed that UIP28 is 97% identical with the rat R B C K I protein [16], UIP48 is 100% identical with the mouse Ariadne-2 protein (accession n u m b e r AJ130975), UIP4 is 95% identical with the product of the h u m a n gene KIAA0161 (accession n u m b e r D79983), UIP5 is 84% identical with the h u m a n KIAA0860 protein (accession n u m b e r AB020667), UIP117 is 59% identical with a mosquito blood meal-induced protein (accession n u m b e r U84248) and UIP77 is 55% identical (73% similar) with the protein encoded by the Drosophila gene Ariadne-I (accessiom n u m b e r X98310). No significant homology was found for UIP83.…”

“…To obtain the cDNA clones pUIP28 and pUIP48, which contain the complete protein coding region for UIP28 and UIP48, respectively, total liver RNA from BALB/c mice was reverse transcribed, as previously described [15], and amplified by PCR using primers derived from the sequence of the UIP28 homologous rat gene RBCK1 [16] or UlP48 homologous EST clones. The amplified fragments were directly cloned into the EcoRV site of the Bluescript vector.…”

RBCK1, an E3 Ubiquitin Ligase, Interacts with and Ubiquinates the Human Pregnane X Receptor

“…RBCK1, RBCC (ring-B-box-coiled-coil) protein interacting with protein kinase C-1 (PKC-1) (C20orf18 or HOIL-1, XAP3, or UIP28), is a transcription factor that consists of a ubiquitin-like sequence (Tokunaga et al, 1998), two coiled-coil regions, a novel zinc finger motif (Meyer et al, 2002), and a RING-IBR (in between RING fingers) domain (Marin and Ferrus, 2002). RBCK1 is localized in both the nucleus and the cytoplasm, possessing a classic Leu-rich nuclear export signal and a nuclear localization signal (Tatematsu et al, 2005).…”

Decoding the Growth Advantage of Hypoxia-Sensitive Lung Cancer

“…LUBAC is a multiprotein complex consisting of HOIL-1L, HOIL-1-interacting protein (HOIP), and Shank-associated RH domain-interacting protein (SHARPIN). The LUBAC component HOIL-1L was also reported to bind PKCz (10), and in some proteins forms K48 polyubiquitin chains for degradation by the 26S proteasome (11,12).…”