Molecular cloning and characterization of two novel transport proteins from rat kidney

Abstract

The recent cloning of renal transport systems for organic anions and cations (OAT1, OCT1, and OCT2) opened the possibility to search, via polymerase chain reaction (PCR) homology screening, for novel transport proteins. Two integral membrane proteins, UST1 and UST2, were cloned from rat kidney. RT-PCR revealed that UST1 is confined to the kidney whereas UST2 mRNA was detected in all tested tissues. Sequence analyses suggest that UST1 and UST2, together with four related transporters, comprise, within the major facilitator superfamily, a so far unrecognized transporter family, termed amphiphilic solute facilitator (ASF) family. Characteristic signatures for the ASF family were identified.z 1998 Federation of European Biochemical Societies.

Molecular Cloning and Characterization of a New Multispecific Organic Anion Transporter from Rat Brain

“…Interaction of bisubstrates with both organic anion and cation transporters may be explained by the structural similarity of the OAT family and OCT family (Table I). Based on these hypothesis, a family including both the OAT and OCT family was proposed (8,25). Now the molecular structures of three organic anion transporters and three organic cation transporters have been revealed.…”

Organic anion transporters: discovery, pharmacology, regulation and roles in pathophysiology

“…The protein shared more identity with Oat1 (39%) than either of the OCTs (o30%) [14]. Expression has been identified only in kidney, and no orthologs have been reported in other species.…”

“…Oat6 and OAT7 are not renally expressed tion (HUGO) Gene Nomenclature Committee has designated the OCT and OAT families as members of the Solute Carrier Superfamily (SLC), and together, they comprise the group, SLC22. Alternatively, they have been referred to as members of the Major Facilitator Superfamily or as Amphiphilic Solute Transporters [13,14].…”

Discovery of the ergothioneine transporter

“…SLC22A4 codes for OCTN1, which belongs to the amphiphilic solute facilitator (ASF) family of integral membrane transporters (3). OCTN1 was independently cloned by us and others some years ago (GenBank accession no.…”