Molecular cloning and characterization of a class II ADP ribosylation factor from the shrimp Marsupenaeus japonicus

Characterization of two novel ADP ribosylation factors from the shrimp Marsupenaeus japonicus

“…In contrast to other arthropods (insects), information about the Arf family in crustaceans was quite limited only one from M. japonicus, designed as MjArf4, have been reported [21]. In the present study, the cDNAs of two Arfs, MjArf1 and MjArfn, were identified from M. japonicus.…”

“…These data indicated that MjArf1 and MjArfn are novel Arf proteins in M. japonicus. MjArf1 and MjArf4 [21] were at the same position in class I and II Arfs in NJ tree respectively, which suggested that the class I and class II Arfs might represent a divergent group from the common ancestor in animals.…”

“…But then, the pathway of secretion or endocytic is easy to be employed to cause viral immune evasion and virus propagation, which Arfs was recruited to the specific target membrane to induce the disintegration of intracellular membranes and formation of specific vesicles by viral proteins mediating their interactors [18,19]. Moreover, Mjcent [20], as the Arf6 GAP, and MjArf4 [21], the member of Arf class II in Marsupenaeus japonicus, were up-regulated after WSSV infection, suggesting that shrimp Arfs may also participate in virus invasion and/or host defense events.…”

“…In the past two decades, a number of Arf proteins and genes have been identified from various organisms, but the only crustacean Arf has been characterized is MjArf4 [21], a member of class II from the shrimp M. japonicus to date. To investigate the different Arfs and the relationships between the Arfs and WSSV infection in the shrimps, here we report two novel Arf proteins, MjArf1 and MjArfn, in M. japonicus and their involvement in the WSSV infection.…”

Cloning and characterization of ADP-ribosylation factor 1b from the olive flounder Paralichthys olivaceus

“…Some documents indicated that small GTPases are related to virus infection in the shrimp (Wu et al 2007;Liu et al 2009;Zhang et al 2010). Also, the small GTPases of zebra fish have provided a firm basis of an innate immune system in vertebrates (Salas-Vidal et al 2005).…”