Molecular cloning and characterization of two phosphatase 2A catalytic subunit genes from Arabidopsis thaliana

Pérez-Callejón, Encarna; Casamayor, Antonio; Pujol, Gemma; Camps, Manel; Ferrer, Albert; Ariño, Joaquı́n

doi:10.1016/s0378-1119(98)00013-4

Cited by 21 publications

(10 citation statements)

References 37 publications

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“…However, the diversity of subunits detected in our experiments argues for a combinatorial diversity of TTP isoforms. We provide evidence that all three A-type, but presumably only two (PP2AC3 and PP2AC4 subunits) of the five Arabidopsis C-type PP2A subunits take part in the complex, the three other PP2AC subunits indeed representing a distinct clade among the five PP2AC subunits 33 . This is further corroborated by the recently published analysis of a complete knock-out double pp2ac3-c4 mutant whose phenotype is strikingly reminiscent of ton1 or fass mutants 34 .…”

Section: Discussionmentioning

confidence: 72%

A protein phosphatase 2A complex spatially controls plant cell division

et al. 2013

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In the absence of cell migration, the orientation of cell divisions is crucial for body plan determination in plants. The position of the division plane in plant cells is set up premitotically via a transient cytoskeletal array, the preprophase band, which precisely delineates the cortical plane of division. Here we describe a protein complex that targets protein phosphatase 2A activity to microtubules, regulating the transition from the interphase to the premitotic microtubule array. This complex, which comprises TONNEAU1 and a PP2A heterotrimeric holoenzyme with FASS as regulatory subunit, is recruited to the cytoskeleton via the TONNEAU1-recruiting motif family of proteins. Despite the acentrosomal nature of plant cells, all members of this complex share similarity with animal centrosomal proteins involved in ciliary and centriolar/centrosomal functions, revealing an evolutionary link between the cortical cytoskeleton of plant cells and microtubule organizers in other eukaryotes.

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Section: Discussionmentioning

confidence: 72%

A protein phosphatase 2A complex spatially controls plant cell division

et al. 2013

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“…By Southern blot analysis, Arin˜o et al (1993) and Casamayor et al (1994) showed that the Arabidopsis genome contains five PP2Ac isogenes; however, only two of the genomic genes, AtPP2A-3 and AtPP2A-4 (which consist of 11 exons and 10 introns) have been described so far (Pe´rez-Callejo´n et al 1998). To retrieve the genomic sequences of the other three Arabidopsis PP2Ac genes (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…IR36) cDNA library (Clontech) was screened by plaque hybridisation with a 1.3-kb barley PP2Ac cDNA probe (unpublished data) and two non-overlapping phage clones, PPcDNA-1 and PPcDNA-5, were obtained. Sequence comparison of the deduced amino acid sequences of PPcDNA-1 (Yu et al 2003) and PPcDNA-5 with the five Arabidopsis PP2Ac isoforms Stamey and Rundle 1995;Pe´rez-Callejo´n et al 1998) suggested that PPcDNA-1 and PPcDNA-5 represent the homologs of two different PP2Ac subfamilies. The deduced protein sequence of PPcDNA-1 shows higher similarity to the Arabidopsis subfamily composed of AtPP2A-1, AtPP2A-2 and AtPP2A-5 (94-95%) than the second subfamily composed of AtPP2A-3 and AtPP2A-4 (77-78%).…”

Section: Isolation Of Three Rice Pp2ac Genesmentioning

confidence: 99%

“…For example, the dicotyledonous plant Arabidopsis thaliana has been shown to contain genes for five isoforms of PP2Ac Casamayor et al 1994;Stamey and Rundle 1995;Pe´rez-Callejo´n et al 1998). The isoforms are divided into two distinct subfamilies (AtPP2A-1, AtPP2A-2 and AtPP2A-5 form the first subfamily while AtPP2A-3 and AtPP2A-4 form the second subfamily) with high degrees of identity between members of the same subfamily (>95%), although identity between subfamilies and indeed mammalian PP2Ac proteins is also high (ca.…”

Section: Introductionmentioning

confidence: 99%

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Structure, evolution and expression of a second subfamily of protein phosphatase 2A catalytic subunit genes in the rice plant (Oryza sativa L.)

Wong

Jack

et al. 2005

Planta

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Protein phosphatase 2A (PP2A) is one of the major serine/threonine protein phosphatases in the cell and plays a variety of regulatory roles in metabolism and signal transduction. Previously, we described the structure and expression of two genes encoding PP2A catalytic subunits (PP2Ac)--OsPP2A-1 and OsPP2A-3--in the rice plant (Yu et al. 2003). Here, we report the isolation and characterisation of a second structurally distinguishable PP2Ac subfamily comprised of three additional isogenes, OsPP2A-2, OsPP2A-4 (each containing ten introns) and OsPP2A-5 (which contains nine introns). Northern blot analysis demonstrated that the three isogenes are ubiquitously expressed in all rice tissues during plant development, and differentially expressed in response to high salinity and the combined stresses of drought and heat. Phylogenetic analyses indicated that the two PP2Ac subfamilies are descended from two ancient lineages, which derived from gene duplications that occurred after the monocotyledon-dicotyledon split. In the second subfamily, it is proposed that two duplication events were involved; in which, the initial duplication of a ten-intron primordial gene yielded OsPP2A-2 and the progenitor of OsPP2A-4 and OsPP2A-5. The OsPP2A-4/OsPP2A-5 progenitor, in turn, underwent a second duplication event, resulting in the present day OsPP2A-4 and OsPP2A-5. It is proposed that loss of the 5'-most intron from OsPP2A-5 occurred after these two duplication events.

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“…Five genes that encode PP2A catalytic subunits (PP2Ac) are found in Arabidopsis, and these five C subunits are grouped into two subfamilies: subfamily I (PP2A-C1, PP2A-C2, and PP2A-C5) and subfamily II (PP2A-C3 and PP2A-C4; Pérez-Callejón et al, 1998). Members of subfamily I are believed to be involved in plant stress and defense responses (He et al, 2004).…”

mentioning

confidence: 99%

Arabidopsis PHOSPHOTYROSYL PHOSPHATASE ACTIVATOR Is Essential for PROTEIN PHOSPHATASE 2A Holoenzyme Assembly and Plays Important Roles in Hormone Signaling, Salt Stress Response, and Plant Development

Chen

Zhu

et al. 2014

Plant Physiology

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PROTEIN PHOSPHATASE 2A (PP2A) is a major group of serine/threonine protein phosphatases in eukaryotes. It is composed of three subunits: scaffolding subunit A, regulatory subunit B, and catalytic subunit C. Assembly of the PP2A holoenzyme in Arabidopsis (Arabidopsis thaliana) depends on Arabidopsis PHOSPHOTYROSYL PHOSPHATASE ACTIVATOR (AtPTPA). Reduced expression of AtPTPA leads to severe defects in plant development, altered responses to abscisic acid, ethylene, and sodium chloride, and decreased PP2A activity. In particular, AtPTPA deficiency leads to decreased methylation in PP2A-C subunits (PP2Ac). Complete loss of PP2Ac methylation in the suppressor of brassinosteroid insensitive1 mutant leads to 30% reduction of PP2A activity, suggesting that PP2A with a methylated C subunit is more active than PP2A with an unmethylated C subunit. Like AtPTPA, PP2A-A subunits are also required for PP2Ac methylation. The interaction between AtPTPA and PP2Ac is A subunit dependent. In addition, AtPTPA deficiency leads to reduced interactions of B subunits with C subunits, resulting in reduced functional PP2A holoenzyme formation. Thus, AtPTPA is a critical factor for committing the subunit A/subunit C dimer toward PP2A heterotrimer formation.

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Molecular cloning and characterization of two phosphatase 2A catalytic subunit genes from Arabidopsis thaliana

Cited by 21 publications

References 37 publications

A protein phosphatase 2A complex spatially controls plant cell division

A protein phosphatase 2A complex spatially controls plant cell division

Structure, evolution and expression of a second subfamily of protein phosphatase 2A catalytic subunit genes in the rice plant (Oryza sativa L.)

Arabidopsis PHOSPHOTYROSYL PHOSPHATASE ACTIVATOR Is Essential for PROTEIN PHOSPHATASE 2A Holoenzyme Assembly and Plays Important Roles in Hormone Signaling, Salt Stress Response, and Plant Development

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