Molecular Cloning and Characterization of the Yew Gene Encoding Squalene Synthase from Taxus cuspidata

Huang, Zheng-Hai; Jiang, Keji; Pi, Yan; Hou, Rong; Liao, Zhihua; Han, Xu; Wang, Qian; Sun, Xiaoping; Tang, Kexuan

doi:10.5483/bmbrep.2007.40.5.625

Cited by 27 publications

(32 citation statements)

References 31 publications

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“…1) with a predicted mass of 47.6 kDa. The result is similar to that of SQSs in other plants, including A. thaliana (Nakashima et al 1995), Taxus cuspidata (Huang et al 2007), and P. ginseng (Kim et al 2011).…”

Section: Discussionsupporting

confidence: 73%

“…Mammals and yeasts have a single copy in their genomes (Robinson et al 1993, Hata et al 1997. Among plant species, a single SQS gene was reported in O. sativa (Hata et al 1997), T. cuspidata (Huang et al 2007), and E. tirucalli (Uchida et al 2009). Likewise, it appears that there is a single copy of the SQS gene in the grain amaranth genome, as suggested by Southern analysis (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Isolation and characterization of a SQS gene from grain amaranth is the first step towards understanding regulation of the squalene biosynthetic pathway. Genes encoding SQS have been isolated from many sources, such as fungi (Jennings et al 1991), bacteria (Lee & Poulter 2008), animals (McKenzie et al 1992), and plants (Nakashima et al 1995, Hata et al 1997, Devarenne et al 1998, Hayashi et al 1999, Akamine et al 2003, Huang et al 2007, Uchida et al 2009, Kim et al 2011). To our knowledge, this is the first report on the molecular cloning and expression analysis of an SQS gene from grain amaranth.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Cloning, Expression and Characterization of a Squalene Synthase Gene from Grain Amaranth (<i>Amaranthus cruentus</i> L.)

Park

Nemoto

Minami

et al. 2016

JARQ

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A gene encoding squalene synthase from grain amaranth was cloned and characterized. The full-length cDNA was 1805-bp long and contained a 1248-bp open reading frame encoding a protein of 416 amino acids with a molecular mass of 47.6 kDa. Southern blot analysis revealed that the A. cruentus genome contained a single copy of the gene. Comparison of the cDNA and genomic sequences indicated that the amaranth SQS gene had 12 introns and 13 exons. All 13 exons contributed to the coding sequence. The predicted amino acid sequence of the SQS cDNA shared high homology with those of SQSs from several other plants. It contained five conserved domains that are believed to represent crucial regions of the active site. We conducted qRT-PCR analyses to examine the expression pattern of the SQS gene in seeds at different developmental stages and in several tissues. The amaranth SQS gene was expressed late in seed development and played a role in the perisperm, and mainly expressed in stem and root tissues.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Molecular Cloning, Expression and Characterization of a Squalene Synthase Gene from Grain Amaranth (<i>Amaranthus cruentus</i> L.)

Park

Nemoto

Minami

et al. 2016

JARQ

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“…A single copy of the SS gene was detected in Euphorbia tirucalli (Uchida et al 2009), Taxus cuspidate (Huang et al 2007) and Oryza sativa (Hata et al 1997), and two copies were detected in N. tabacum (Devarenne et al 1998), A. thaliana (Busquets et al 2008) and G. glabra (Hayashi et al 1999). In B. falcatum, BfSS1 gene was predicted to contain two copies.…”

Section: Discussionmentioning

confidence: 95%

Gene regulation patterns in triterpene biosynthetic pathway driven by overexpression of squalene synthase and methyl jasmonate elicitation in Bupleurum falcatum

Kim

Cho

Park

et al. 2010

Planta

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The root of Bupleurum falcatum L. (Apiaceae) has long been one of the most important traditional herbal medicines in Asian countries. A group of triterpene saponins (saikosaponins) are the major constituents of this plant. Squalene synthase (SS) may play a regulatory role in directing triterpene intermediates and sterol pathways. Here, we investigated the regulatory role of the squalene synthase (BfSS1) gene in the biosynthesis of phytosterol and triterpene in B. falcatum. BfSS1 mRNA accumulated ubiquitously in plant organs and markedly increased in roots after treatment with methyl jasmonate (MeJA), ABA and ethephon. Transgenic B. falcatum constructs overexpressing BfSS1 in the sense and antisense orientations were assembled using the Agrobacterium-mediated method. Transgenic roots overexpressing BfSS1 in the sense orientation resulted in enhanced production of both phytosterol and saikosaponins. Overexpression of the BfSS1 gene in the sense orientation increased the mRNA accumulation of downstream genes such as squalene epoxidase and cycloartenol synthase but unexpectedly decreased the mRNA levels of β-amyrin synthase (β-AS), a triterpene synthase mRNA. MeJA treatment of wild-type roots strongly stimulated β-AS mRNA accumulation and saikosaponin production but suppressed phytosterol production. MeJA treatment of transgenic roots overexpressing BfSS1 in the sense orientation failed to stimulate β-AS mRNA accumulation but still enhanced saikosaponin and phytosterol production. These results indicate that overexpression of BfSS1 in B. falcatum regulates more powerfully the downstream genes than elicitor (MeJA) treatment in triterpene and phytosterol biosynthesis.

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“…Biosynthesis of taxol and squalene-derived products shared the same isoprenoid biosynthetic pathway upon the precursor farnesyl diphosphate (FPP) (Huang et al, 2007). Decreasing the carbon flux through competitive pathways is shown to be one of the useful approaches to improvement of the desired secondary metabolism (Oksman-Caldentey and Inze, 2004).…”

Section: Discussionmentioning

confidence: 99%

Squalestatin-induced production of taxol and baccatin in cell suspension culture of yew (Taxus baccata L.)

Amini¹,

Shabani²,

Afghani³

et al. 2014

Turk J Biol

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Various elicitors have already been reported to enhance the production of taxol and related taxanes. In this study, effects of a fungal metabolite, squalestatin, on production of taxol and baccatin III were studied. Expressions of 4 key involved genes, ts, dbat, bapt, and dbtnbt, in suspension cultures of Taxus baccata were also detected using qPCR. Results showed that application of squalestatin significantly increased taxol and baccatin III yields. Increased expressions of the genes were in accordance with measures of taxol and baccatin accumulations in cells and medium. Production of H 2 O 2 has significant positive correlations with both gene expression and taxanes, indicating that the increase in H 2 O 2 might be involved in the upregulation of the taxane production in yew under squalestatin treatment. Our results suggest that H 2 O 2 is a key signaling component in the stimulation of taxane production in T. baccata cells induced by squalestatin.

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Molecular Cloning and Characterization of the Yew Gene Encoding Squalene Synthase from Taxus cuspidata

Cited by 27 publications

References 31 publications

Molecular Cloning, Expression and Characterization of a Squalene Synthase Gene from Grain Amaranth (<i>Amaranthus cruentus</i> L.)

Molecular Cloning, Expression and Characterization of a Squalene Synthase Gene from Grain Amaranth (<i>Amaranthus cruentus</i> L.)

Gene regulation patterns in triterpene biosynthetic pathway driven by overexpression of squalene synthase and methyl jasmonate elicitation in Bupleurum falcatum

Squalestatin-induced production of taxol and baccatin in cell suspension culture of yew (Taxus baccata L.)

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