Molecular cloning and characterization of rho, a ras-related small GTP-binding protein from the garden pea.

Yang, Zhenbiao; Watson, John C.

doi:10.1073/pnas.90.18.8732

Cited by 122 publications

(65 citation statements)

References 35 publications

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“…), all plant Rho-like GTPases fall into a single subfamily, ROP (Figure 1) (Brembu et al 2006,Burridge & Wennerberg 2004,Yang 2002,Yang & Fu 2007,Yang & Watson 1993. Six (ROP1 to ROP6) of the 11 Arabidopsis ROPs are known to regulate cell polarity formation (Gu et al 2004,Yang 2002.…”

Section: Rop Gtpases and Partners: Plant Reinvention Of Conserved Polmentioning

confidence: 99%

Cell Polarity Signaling in Arabidopsis

Yang

2008

Annu. Rev. Cell Dev. Biol.

253

239

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Cell polarization is intimately linked to plant development, growth, and responses to the environment. Major advances have been made in our understanding of the signaling pathways and networks that regulate cell polarity in plants owing to recent studies on several model systems, e.g., tip growth in pollen tubes, cell morphogenesis in the leaf epidermis, and polar localization of PINs. From these studies we have learned that plant cells use conserved mechanisms such as Rho family GTPases to integrate both plant-specific and conserved polarity cues and to coordinate the cytoskeketon dynamics/reorganization and vesicular trafficking required for polarity establishment and maintenance. This review focuses upon signaling mechanisms for cell polarity formation in Arabidopsis, with an emphasis on Rho GTPase signaling in polarized cell growth and how these mechanisms compare with those for cell polarity signaling in yeast and animal systems.

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Section: Rop Gtpases and Partners: Plant Reinvention Of Conserved Polmentioning

confidence: 99%

Cell Polarity Signaling in Arabidopsis

Yang

2008

Annu. Rev. Cell Dev. Biol.

253

239

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“…A number of ROPs have been reported from various plant species (Yang and Watson, 1993;Bischoff et al, 1999;Gu et al, 2004). The Arabidopsis thaliana genome encodes 11 ROP genes categorized into four phylogenetic groups, which share high homology.…”

Section: Introductionmentioning

confidence: 99%

“…Plants possess a distinct family of Rho GTPases, named ROP (Yang and Watson, 1993;Winge et al, 1997), but apparently lack orthologs of Cdc42, Rac, and Rho. A number of ROPs have been reported from various plant species (Yang and Watson, 1993;Bischoff et al, 1999;Gu et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis Small G Protein ROP2 Is Activated by Light in Guard Cells and Inhibits Light-Induced Stomatal Opening

Jeon

Hwang

et al. 2008

The Plant Cell

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ROP small G proteins function as molecular switches in diverse signaling processes. Here, we investigated signals that activate ROP2 in guard cells. In guard cells of Vicia faba expressing Arabidopsis thaliana constitutively active (CA) ROP2 fused to red fluorescent protein (RFP-CA-ROP2), fluorescence localized exclusively at the plasma membrane, whereas a dominant negative version of RFP-ROP2 (DN-ROP2) localized in the cytoplasm. In guard cells expressing green fluorescent protein-ROP2, the relative fluorescence intensity at the plasma membrane increased upon illumination, suggesting that light activates ROP2. Unlike previously reported light-activated factors, light-activated ROP2 inhibits rather than accelerates light-induced stomatal opening; stomata bordered by guard cells transformed with CA-rop2 opened less than controls upon light irradiation. When introduced into guard cells together with CA-ROP2, At RhoGDI1, which encodes a guanine nucleotide dissociation inhibitor, inhibited plasma membrane localization of CA-ROP2 and abolished the inhibitory effect of CA-ROP2 on light-induced stomatal opening, supporting the negative effect of active ROP2 on stomatal opening. Mutant rop2 Arabidopsis guard cells showed phenotypes similar to those of transformed V. faba guard cells; CA-rop2 stomata opened more slowly and to a lesser extent, and DN-rop2 stomata opened faster than wild-type stomata in response to light. Moreover, in rop2 knockout plants, stomata opened faster and to a greater extent than wild-type stomata in response to light. Thus, ROP2 is a light-activated negative factor that attenuates the extent of light-induced changes in stomatal aperture. The inhibition of light-induced stomatal opening by light-activated ROP2 suggests the existence of feedback regulatory mechanisms through which stomatal apertures may be finely controlled.

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“…According to the postulated functions of these plant proteins, this superfamily is subdivided into three subfamilies that represent (i) the membrane-bound Rab/Ypt-type-proteins involved in regulating vesicular trafficking (Terryn et al, 1993), (ii) the membrane-bound Rho/Rac-type proteins thought to be important for cytoskeleton organization (Yang and Watson, 1993) and (iii) the soluble Ran (Rasrelated nuclear protein) that is most probably necessary for protein import into the nucleus and for the onset of mitosis (Ach and Gruissem, 1994;Merkle et al, 1994). The mammalian Ran cDNA, originally identified as TC4 (Drivas eta/., 1990) codes for a 25 kDa nuclear protein and has been shown to bind to the chromatin-associated protein RCC1 (Bischoff and Ponstingl, 1991a).…”

Section: Introductionmentioning

confidence: 99%