Molecular cloning and characterisation of SaCLCa1, a novel protein of the chloride channel (CLC) family from the halophyte Suaeda altissima (L.) Pall

Nedelyaeva, O.I.; Shuvalov, Alexey; Karpichev, Igor V.; Беляев, Д.В.; Myasoedov, N. A.; Халилова, Л. А.; Khramov, Dmitrii E.; Попова, Л. Г.; Balnokin, Yu. V.

doi:10.1016/j.jplph.2019.152995

Cited by 11 publications

(35 citation statements)

References 49 publications

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“…SaCLC‐a1 possesses both conserved glutamates (gating glutamate and proton glutamate) and proline in the selectivity filter, suggesting that it may have NO 3 − /H + activity as AtCLC‐a and AtCLC‐b. Although SaCLC‐a1 failed to complement Δ gef1 mutant, substituting proline with serine (P188S) in the selectivity filter conferred partial growth restoration of Δ gef1 cells (Nedelyaeva et al ., 2019). These studies imply that orthologs of AtCLC‐a and AtCLC‐b in other plant species most probably possess a conserved function as NO 3 − /H + transporter.…”

Section: Clcs In Plantsmentioning

confidence: 99%

“…In Suaeda altissima , the expression of SaCLC‐c1 (homolog of AtCLC‐c ) increases in leaves with a rise in Cl − concentrations (Nedelyaeva et al ., 2019). SaCLC‐c1 possesses both gating and proton glutamates, and serine in the selectivity filter, indicating its role as Cl − /H + antiporters (Nedelyaeva et al ., 2019). When expressed in gef1 yeast mutant, SaCLC‐c1 complemented the iron deficient, pH‐sensitive, as well as salt‐sensitive growth phenotype of the mutant, supporting SaCLC‐c1 as a chloride transporter (Nedelyaeva et al ., 2018, 2019).…”

Section: Clcs In Plantsmentioning

confidence: 99%

“…SaCLC‐c1 possesses both gating and proton glutamates, and serine in the selectivity filter, indicating its role as Cl − /H + antiporters (Nedelyaeva et al ., 2019). When expressed in gef1 yeast mutant, SaCLC‐c1 complemented the iron deficient, pH‐sensitive, as well as salt‐sensitive growth phenotype of the mutant, supporting SaCLC‐c1 as a chloride transporter (Nedelyaeva et al ., 2018, 2019). However, AtCLC‐c does not suppress the growth phenotype of the gef1 yeast mutant (Hechenberger et al ., 1996).…”

Section: Clcs In Plantsmentioning

confidence: 99%

“…BnaCLC‐b was induced in the shoots by NO 3 , while BnaCLC‐c to ‐ g members were either down‐regulated or showed no changes to NO 3 − treatment (Liao et al ., 2018). Even in Suaeda altissima , a euhalophyte plant, the expression of SaCLC‐a1 (that shares sequence similarity with AtCLC‐a and AtCLC‐b ) increased in the roots in response to nitrate‐deficiency (Nedelyaeva et al ., 2019). SaCLC‐a1 possesses both conserved glutamates (gating glutamate and proton glutamate) and proline in the selectivity filter, suggesting that it may have NO 3 − /H + activity as AtCLC‐a and AtCLC‐b.…”

Section: Clcs In Plantsmentioning

confidence: 99%

Section: Clcs In Plantsmentioning

confidence: 99%

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The chloride channels: Silently serving the plants

Subba

Tomar

Pareek

et al. 2020

Physiologia Plantarum

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Chloride channels (CLCs), member of anion transporting proteins, are present ubiquitously in all life forms. Diverging from its name, the CLC family includes both channel and exchanger (proton‐coupled) proteins; nevertheless, they share conserved structural organization. They are engaged in diverse indispensable functions such as acid and fluoride tolerance in prokaryotes to muscle stabilization, transepithelial transport, and neuronal development in mammals. Mutations in genes encoding CLCs lead to several physiological disorders in different organisms, including severe diseases in humans. Even in plants, loss of CLC protein function severely impairs various cellular processes critical for normal growth and development. These proteins sequester Cl− into the vacuole, thus, making them an attractive target for improving salinity tolerance in plants caused by high abundance of salts, primarily NaCl. Besides, some CLCs are involved in NO3− transport and storage function in plants, thus, influencing their nitrogen use efficiency. However, despite their high significance, not many studies have been carried out in plants. Here, we have attempted to concisely highlight the basic structure of CLC proteins and critical residues essential for their function and classification. We also present the diverse functions of CLCs in plants from their first cloning back in 1996 to the knowledge acquired as of now. We stress the need for carrying out more in‐depth studies on CLCs in plants, for they may have future applications towards crop improvement.

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Section: Clcs In Plantsmentioning

confidence: 99%

Section: Clcs In Plantsmentioning

confidence: 99%

Section: Clcs In Plantsmentioning

confidence: 99%

Section: Clcs In Plantsmentioning

confidence: 99%

Section: Clcs In Plantsmentioning

confidence: 99%

See 3 more Smart Citations

The chloride channels: Silently serving the plants

Subba

Tomar

Pareek

et al. 2020

Physiologia Plantarum

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Molecular control to salt tolerance mechanisms of woody plants: recent achievements and perspectives

Llanes

Palchetti

Ibáñez

2021

Annals of Forest Science

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Key message Woody plants have salt-tolerant mechanisms similar to those developed by non-woody plants. Among others, compartmentalization of ions, production of compatible solutes, synthesis of specific proteins and metabolites, and induction of transcriptional factors are the most relevant. Woody plant-associated microbial interactions as well as naturally stress-adapted trees are resources that deserve to be deepened to fully understand the tolerance mechanisms. Context The high variability of salinity responses found in woody plants implies a high potentiality for germplasm selection and breeding. Salt tolerance mechanisms of plants are regulated by numerous genes, which control ion homeostasis, production of compatible solutes and specific proteins, and activation or repression of specific transcription factors. Despite the fact that numerous studies have been done on herbaceous model plants, knowledge about salt tolerance mechanisms in woody plants is still scarce. Aims The present review critically evaluates molecular control of salt tolerance mechanisms of woody plants, focusing on the regulation and compartmentalization of ions, production of compatible solutes, activation of transcription factors, and differential expression of stress response-related proteins, including omics-based approaches and the role of plant-microbial interactions. The potential identification of genes from naturally stress-adapted woody plants and the integration of the massive omics data are also discussed. Conclusion In woody plants, salt tolerance mechanisms seem not to diverge to those identified in non-woody plants. More comparative studies between woody and non-woody salt tolerance plants will be relevant to identify potential molecular mechanisms specifically developed for wood plants. In this sense, the activation of metabolic pathways and molecular networks by novel genetic engineering techniques is key to establish strategies to improve the salt tolerance in woody plant species and to contribute to more sustainable agricultural and forestry systems.

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