Molecular Cloning and Characterisation of Farnesyl Pyrophosphate Synthase from Tripterygium wilfordii

Abstract

Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophosphate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli. Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment. The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.

De Novo Transcriptome and Expression Profile Analysis to Reveal Genes and Pathways Potentially Involved in Cantharidin Biosynthesis in the Blister Beetle Mylabris cichorii

“…Farnesyl diphosphate (FPP) is the precursor of sesquiterpenoids [ 36 , 37 ]. We identified the key enzyme gene farnesyl diphosphate synthase ( FPPS ; 2.5.1.1) in our library and found that it was significantly up-regulated (unigene16392) in male compared with female beetles, while the gene for isopentenyl-diphosphate delta-isomerase ( IDI ; 5.3.3.2), another important enzyme in terpenoid biosynthesis, was also significantly up-regulated (CL3032.…”

Comparative proteomic analyses reveal the proteome response to short-term drought in Italian ryegrass (Lolium multiflorum)

“…Terpenoids constitute a large and structurally diverse group of chemicals, playing diverse functional roles in plants as hormones, electron carriers and structural component of membranes [ 48 ]. Farnesyl pyrophosphate synthase (FPS) not only played a vital role in terpenoid metabolism, but also functioned as a key regulatory enzyme to control the sterol biosynthetic pathway [ 49 ]. In our study, FPS as key regulators was significantly accumulated and involved in Terpenoid backbone biosynthesis.…”

Genetic Transformation System for Woody Plant Tripterygium wilfordii and Its Application to Product Natural Celastrol

“…Indeed, synthetic biology has been designed, regulated and optimized on different levels to produce new drugs, biofuels, and target products to maximize production ( Gechev et al, 2014 ). In recent years, terpenoid biosynthetic pathway analysis has been the most important part of the research in T. wilfordii ( Li et al, 2009 ; Wu et al, 2012 ; Liu et al, 2014 ; Zhang et al, 2015 ; Zhao et al, 2015 ). Our ongoing studies were focus on identifying preciously uncharacterized related genes in terpenoids metabolism of T. wilfordii .…”