Molecular cloning and characterisation of Ts8B1, Ts8B2 and Ts8B3, three new members of the Taenia solium metacestode 8kDa diagnostic antigen family

Ferrer, Elizabeth; Bonay, Pedro; Foster-Cuevas, Mildred; González, Luís Miguel; Dávila, Iris; Cortéz, María Milagros; Harrison, L.J.S.; Parkhouse, R. M. E.; Gárate, Teresa

doi:10.1016/j.molbiopara.2006.12.003

Cited by 37 publications

(27 citation statements)

References 43 publications

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“…Significantly, specificities of the HP6-GST and HP6-Bac are higher than the one showed by cyst fluid and other recombinant metacestode antigens (Chung et al 1999;Hubert et al 1999;Sako et al 2000;Greene et al 2000;Hancock et al 2003) and similar specificity to the metacestode-derived antigen Ts8B2 (Ferrer et al 2007). Therefore, this oncosphere derived molecule HP6 may be of value in the diagnosis of human cysticercosis, particularly when expressed in the baculovirus system, considering its better sensitivity with inactive NCC patient samples.…”

Section: Discussionmentioning

confidence: 87%

“…metacestodes are useful in the diagnosis of cysticercosis (Tsang et al 1989;Ito et al 1998), but the enzyme-linked immunoelectrotransfer blot technology is labourious, expensive and requires parasite material. More economical and practical solutions are actively being sought (for example Hancock et al 2003Hancock et al , 2004Ferrer et al 2003Ferrer et al , 2005aFerrer et al , 2007Fleury et al 2003) as they could be of value particularly in extensive epidemiological surveys in endemic areas.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, there is a continuing need for alternative, practical, reproducible and economic supportive diagnostic methodologies that avoid the use of crude parasite material (Chung et al 1999;Greene et al 2000;Hancock et al 2003;Ferrer et al 2003Ferrer et al , 2005aFerrer et al , 2007.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis

et al. 2007

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With the objective of providing inexpensive and reproducible assays for the detection of antibodies indicating exposure to Taenia saginata and Taenia solium, we have evaluated the diagnostic utility of the T. saginata oncosphere adhesion protein (HP6-Tsag), expressed in baculovirus (HP6-Bac) and bacteria (HP6-GST [glutathione S-transferase]), employing enzyme-linked immunosorbent assays (ELISAs) and sera from T. saginata infected cattle, T. solium infected pigs and serum and cerebrospinal fluid (CSF) samples from clinically defined T. solium neurocysticercosis (NCC) patients. The two recombinant proteins were antigenic in all three systems, with the signal to background ratio of the HP6-Bac ELISA slightly higher than that for the HP6-GST ELISA. Assay performance in cattle was similar to previously described peptide-based ELISA assays, although NCC sample sensitivity/specificity was marginally better. The sensitivity of the HP6-Bac and HP6-GST ELISAs was close for active human NCC (77.4 and 80.6% for serum and 76.9 and 73.1% for CSF samples, respectively). In inactive human NCC, however, the sensitivity of the HP6-Bac ELISA was almost twice that of the HP6-GST ELISA. Because peptides are relatively expensive and recombinant proteins are simple and economical to produce, the latter may provide useful reagents for antibody detection in countries with endemic cysticercosis/NCC.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis

et al. 2007

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“…68 Other diagnostic candidates in the 8 kDa family have been expressed and produced as recombinant proteins including Ts8B1, Ts8B2, and Ts8B3. Among these, the Ts8B2 antigen was better able to discriminate between cases of active and inactive NCC, 72 although some cross-reactions with echinococcosis and schistosomiasis were observed.…”

Section: -24mentioning

confidence: 99%

Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

126

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Cysticercosis, the infection with the larval stage of Taenia solium, is a cause of neurological symptoms including seizures, affecting the quality of life of patients and their families. Diagnosis focuses on brain imaging and serological tests are mostly used as confirmatory tools. Most cases, however, occur in poor endemic areas, where both kinds of diagnostic tools are poorly available. Development of point of care diagnostic tests is one of the most important priorities for cysticercosis researches today. The ideal point of care test would require detection of viable cysticercosis and hopefully identify cases with severe or progressive forms of neurocysticercosis, leading to referral of the patient for specialized medical attention. This manuscript describes the evolution of the serological diagnosis of cysticercosis over time, and the characteristics of the most common currently available tools, their advantages and disadvantages, and their potential use in future diagnostic tests.

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“…The use of other recombinant antigens (54,96) or synthetic peptides (100), examination of isotypes such as IgG4 (55), the use of tertiary conformational alterations (227) or multiepitope chimeric proteins (245) and other novel strategies may further improve immunological detection methods.…”

Section: Diagnosismentioning

confidence: 99%

Update on Eosinophilic Meningoencephalitis and Its Clinical Relevance

2009

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SUMMARY Eosinophilic meningoencephalitis is caused by a variety of helminthic infections. These worm-specific infections are named after the causative worm genera, the most common being angiostrongyliasis, gnathostomiasis, toxocariasis, cysticercosis, schistosomiasis, baylisascariasis, and paragonimiasis. Worm parasites enter an organism through ingestion of contaminated water or an intermediate host and can eventually affect the central nervous system (CNS). These infections are potentially serious events leading to sequelae or death, and diagnosis depends on currently limited molecular methods. Identification of parasites in fluids and tissues is rarely possible, while images and clinical examinations do not lead to a definitive diagnosis. Treatment usually requires the concomitant administration of corticoids and anthelminthic drugs, yet new compounds and their extensive and detailed clinical evaluation are much needed. Eosinophilia in fluids may be detected in other infectious and noninfectious conditions, such as neoplastic disease, drug use, and prosthesis reactions. Thus, distinctive identification of eosinophils in fluids is a necessary component in the etiologic diagnosis of CNS infections.

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Molecular cloning and characterisation of Ts8B1, Ts8B2 and Ts8B3, three new members of the Taenia solium metacestode 8kDa diagnostic antigen family

Cited by 37 publications

References 43 publications

Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis

Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis

Immunological and molecular diagnosis of cysticercosis

Update on Eosinophilic Meningoencephalitis and Its Clinical Relevance

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