Molecular cloning–A laboratory manual. New York: Cold Spring Harbor Laboratory. 1982, 545 S., 42 $

Mamiatis, T.; Fritsch, Elisabeth; Sambrook, Joseph; Engel, Jonathan

doi:10.1002/abio.370050118

Cited by 3,604 publications

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“…DNA samples were prepared from peripheral blood with the standard technique (Maniatis et al, 1982). Four of seven probes were purchased from Amersham, U.K. MR24/1 XY pseudoautosomal DNA probe (MR24/1) is assigned to the pseudoautosomal region at the telomers of X and Y chromosomes.…”

Section: Methodsmentioning

confidence: 99%

Investigation of paternity establishing without the putative father using hypervariable DNA probes

et al. 1990

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SummarySeven kinds of DNA probes which recognize hypervariable loci were applied for paternity test. The putative father was decreased and unavailable for the test. The two legitimate children and their mother (the deceased's wife) and the four illegitimate children and their mother (the deceased's kept mistress) were available for analysis. Paternity index of four illegitimate child was investigated. Allelic frequencies and their confidence intervals among unrelated Japanese individuals were previously reported from our laboratory, and co-dominant segregation of the polymorphism was confirmed in family studies. Cumulative paternity indices of four illegitimate children from 16 kinds of standard blood group markers were 165, 42, 0.09, and 36, respectively. On the other hand, cumulative paternity indices from 7 kinds of DNA probes are 2, 363, 4,685, 57,678, and 54,994, respectively, which are 14, 113, 640, 864, and 1,507 times higher than that from standard blood group markers. The DNA analyses gave nearly conclusive evidence that the putative father was the biological father of the children. Especially, the paternity relation of the third illegitimate child could not be established without the DNA analyses. Accordingly, DNA polymorphism is considered to be informative enough for paternity test.

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Section: Methodsmentioning

confidence: 99%

Investigation of paternity establishing without the putative father using hypervariable DNA probes

et al. 1990

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“…To prepare DNA from peripheral blood, buffy coat was mixed with several aliquots of 0.2~ NaC1 containing 10 mM ethylenediamine tetraacetic acid (EDTA) at 4~ DNA was extracted by the standard technique (Maniatis et at., 1982). Blood and semen stains were prepared on cotton fabric and aged at room temperature for one month.…”

Section: Methodsmentioning

confidence: 99%

“…Standard procedures were used for bacterial transformation and plasmid DNA isolation (Maniatis et al, 1982). Y specific probe (pHY10) was labeled with [a2p]dCTP (3,000 ~Ci/mmol) using a Multiprime DNA labeling system (Amersham).…”

Section: Methodsmentioning

confidence: 99%

Hypervariable polymorphism of autosomal origin detected by the Y-chromosome derived probe, pHY10

Yokoi

Sagisaka

Nakahori³

et al. 1989

Jap J Human Genet

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SummaryA recombinant DNA probe (pHY10) hybridizing specifically to human DNA family DYZ1, 3,000 copies of which are present on the long arm of the Y chromosome, was used for probing human genome DNA digested with various restriction enzymes. To our surprise, the probe detected a hypervariable polymorphism of autosomal origin in human DNA when digested with TaqI. None of other 12 restriction enzymes revealed polymorphic patterns. Codominant segregation of the polymorphism was established in family studies. This probe has been widely used in the detection of the Y chromosome. Its ease of availability as well as highly discriminating polymorphic pattern makes it potentially very useful for forensic and human genetic purposes.

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“…Once adapted, an overnight culture was diluted 1:lOO into 20 mL of M9 (Maniatis et al, 1982) supplemented with 0.12% glucose (0.5 mCi [U-'4C]glucose, Amersham), 2 mM MgS04, 0.1 mg/mL ampicillin, and incubated at 30 "C until a Klett reading of 150 was obtained. Thioredoxin was induced by shifting the temperature to 43 "C and continuing growth for 3 h. Metabolically released I4CO2 was trapped in two sequential traps containing 2 M sodium hydroxide.…”

Section: Thioredoxin Purificationmentioning

confidence: 99%

Differences in hydrogen exchange behavior between the oxidized and reduced forms of Escherichia coli thioredoxin

Kaminsky

Richards

1992

Protein Science

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Amide proton exchange of thioredoxin is used to monitor the structural effects of reduction of its single disulfide. An effective 3–5‐proton difference between the oxidized and reduced protein form is observed early in proton out‐exchange of the whole protein, which is independent of temperature in the range of 5–45 °C, indicating that redox‐sensitive changes are probably not due to low‐energy structural fluctuations. Medium resolution hydrogen exchange experiments have localized the redox‐sensitive amide protons to two parts of the sequence that are distant from each other in the three‐dimensional structure: the active‐site turn and the first β‐strand. The sum of the proton differences observed in the peptides from these regions is equal to that of the whole protein, indicating that all redox‐sensitive hydrogen exchange effects are observed in the peptide experiments. A model combining structural changes within the protein matrix with changes in the surface hydration properties is proposed as a mechanism for the communication between distant sites within the protein. Sound velocity and density measurements of reduced and oxidized thioredoxin are presented in the accompanying paper (Kaminsky, S.M. & Richards, F.M., 1992, Protein Sci. 1, 22–30).

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Molecular cloning–A laboratory manual. New York: Cold Spring Harbor Laboratory. 1982, 545 S., 42 $

Cited by 3,604 publications

References 0 publications

Investigation of paternity establishing without the putative father using hypervariable DNA probes

Investigation of paternity establishing without the putative father using hypervariable DNA probes

Hypervariable polymorphism of autosomal origin detected by the Y-chromosome derived probe, pHY10

Differences in hydrogen exchange behavior between the oxidized and reduced forms of Escherichia coli thioredoxin

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