Molecular clonality and antimicrobial resistance in Salmonella entericaserovars Enteritidis and Infantis from broilers in three Northern regions of Iran

Rahmani, Maral; Peighambari, Seyed Mostafa; Svendsen, Christina Aaby; Cavaco, Lina; Agersø, Yvonne; Hendriksen, René S.

doi:10.1186/1746-6148-9-66

Cited by 52 publications

(52 citation statements)

References 41 publications

(40 reference statements)

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“…from animal and human by PFGE as well as the other methods, proved that PFGE is probably the most effective molecular technique (5). Similarly, in recent work in Iran, Rahmani et al in 2013 reported that the results obtained from serotyping and PFGE patterns are practical for determining the current distribution of MDR serotypes of Salmonella and epidemiological state of isolates circulating among poultry (23). These two results were the same as our results.…”

Section: Discussionsupporting

confidence: 89%

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Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran

Golab¹,

Khaki

Noorbakhsh³

2014

Int J Enteric Pathog

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Background: Salmonella is one of the most widespread zoonotic enter pathogenic microorganisms found in the global food chain. Poultry and Poultry products have been identified as one of the important foodborne sources of Salmonella. Pulsed-Field Gel Electrophoresis (PFGE) is a gold standard typing method for identification of Salmonella isolates during outbreaks and epidemiological investigations. Objectives: The aim of this study was to carry out molecular typing of Salmonella enterica spp. by PFGE technique. Materials and Methods: All 47 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates were analyzed by means of the molecular technique XbaI PFGE. Results: In the current work, PFGE and serotyping were used to subtype 47 Salmonella isolates belonging to 22 different serotypes and derived from poultry. Thirty-nine PFGE patterns out of 47 isolates were obtained. The Discrimination Index (DI) by serotyping (0.93) was lower than PFGE (DI = 0.99). Conclusions:In conclusion, molecular methods such as PFGE can be used for epidemiological characterization of Salmonella serotypes.

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Section: Discussionsupporting

confidence: 89%

“…in poultry in this country (5,23). Therefore, our study was to do molecular typing of the Salmonella serotypes in poultry by PFGE technique.…”

Section: Discussionmentioning

confidence: 99%

Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran

Golab¹,

Khaki

Noorbakhsh³

2014

Int J Enteric Pathog

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“…[30][31][32] Our study indicated that Salmonella isolates were most frequently resistant to tetracycline (77.12%), sulfisoxazole (66.62%), and nalidixic acid (64.74%), and this was consistent with the findings of other studies. 33,34 This study presented that 92.92% of the class I integron-positive isolates were resistant to at least two antibiotics, and this exhibited a good evidence for the relationship between the presence of class I integron and the emerging of MDR in gram-negative bacteria. 12,29 Integron, a mobile DNA element, is known to contain one or more linked antimicrobial resistance genes and can transfer these genes among bacteria.…”

Section: Discussionmentioning

confidence: 82%

Antibiotic Susceptibility and Molecular Screening of Class I Integron in Salmonella Isolates Recovered from Retail Raw Chicken Carcasses in China

Meng

Zhang

et al. 2017

Microbial Drug Resistance

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Salmonella is one of the leading causes for foodborne diseases. Foods, particularly those of animal origin, act as an important role for Salmonella transmission. In this study, the antibiotic susceptibility of 743 Salmonella isolates recovered from retail raw chicken carcasses in eight provinces was tested, and the isolates were also screened for the presence of class I integron and drug-resistant gene cassettes. One hundred thirteen (15.21%) isolates were harboring class I integron. A higher percentage of integron-positive Salmonella isolates were found in retail chicken in Sichuan Province (29.33%), followed by Beijing (22.14%), Shaanxi (19.15%), Guangxi (14.13%), Henan (12.50%), Shanghai (7.25%), Fujian (8.22%), and Guangdong (6.25%) Provinces. The respective prevalence of class I integron in Salmonella isolates recovered from retail chickens in large, free, and small markets was 16.31%, 14.04%, and 15.27%. Moreover, 20.13%, 14.02%, and 13.74% of Salmonella isolates recovered from retail chickens stored in frozen, chilled, and ambient conditions, respectively, were positive for class I integron. Subsequent sequencing of class I integron revealed the presence of 10 gene cassettes harboring resistance genes (dfrA17-aadA5, dfrA17-aadA5, dfrA1-aadA1, dfrA12-aadA2, dfrA17-aadA5-aadA4, dfrA1-aadA1-aadA2, dfrA1, dfrA5, aadA2, aacA4-catB8-aadA1-dfrA1-(aac6-II)-(bla-8), bla-bla). The most prevalent gene cassette was dfrA17-aadA5 (59.62%). Class I integron-positive isolates were significantly more resistant to multiple antibiotics, and they commonly exhibited corresponding antibiotic resistance profiles to the antibiotic resistance gene cassettes harbored in their class I integron. The results indicated that class I integron with different antibiotic resistance gene cassettes that were prevalent in Salmonella isolates differed from provinces, marketplaces, and chicken storage conditions.

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“…Resistance to ciprofloxacin was reported to be 66.7% to 100%, especially in Germany, Slovakia, Bulgaria and Austria [4] , while the study strains showed sensitivity to ciprofloxacin. Also high frequency of resistance to ciprofloxacin and nalidixic acid for S. Infantis was reported in several reports in Iran too [22,23] . Quinolones were used frequently in treatment regimens and also as a feed additives in poultry industry [24] .…”

Section: Discussionmentioning

confidence: 97%

Class 1 Integrons and the Antibiotic Resistance Profile of Salmonella Infantis Strains from Broiler Chickens

Kaya¹,

Yapıcıer²,

Akan³

et al. 2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleKaya İB, Şahan Yapıcıer Ö, Akan M, Diker KS: Class 1 integrons and the antibiotic resistance profile of Salmonella infantis strains from broiler chickens. Kafkas Univ Vet Fak Derg, 23 (5): 803-807, 2017803-807, . DOI: 10.9775/kvfd.2017 Abstract Salmonella infections are one of the most important diseases and cause economic problems in poultry. The zoonotic feature of the agent and leading to food-borne infections are also important in public health issues. Increasing antibiotic resistance causes difficulties of controlling Salmonella infections, in recent years. Among non-typhoidal Salmonella serotypes the rate of Salmonella Infantis are increasing in Turkey. With this increase, it is important to know the antimicrobial resistance patterns of Salmonella Infantis strains as seen in other Salmonella serotypes. In this study, we aimed to investigate Salmonella Infantis strains which were isolated from feces of healthy broiler chickens for the presence of antibiotic resistance and frequency of Class 1 integrons that is responsible for the transfer of antibiotic resistance as plasmids, transposons. For this purpose a total of 150 S. Infantis strains which were isolated and identified according to the ISO 6579-2002 and Kauffmann-White serotyping scheme were used. Antimicrobial resistance of the strains was determined by the disc diffusion method following to the recommendations of CLSI 2011 standard protocol and also Class 1 integrons were investigated by PCR. According to the results, high rate of multi-drug antibiotic resistance (89.3%), high rate of sensitivity (100%) to cefotaxime, ciprofloxacin, gentamicin, ceftazidime were observed and also Class 1 integrons were determined in all isolates. In conclusion, the presence of Class 1 integron is all strains of Salmonella Infantis showed the potential importance of these strains as recipient for antibiotic resistance.

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Molecular clonality and antimicrobial resistance in Salmonella entericaserovars Enteritidis and Infantis from broilers in three Northern regions of Iran

Cited by 52 publications

References 41 publications

Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran

Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran

Antibiotic Susceptibility and Molecular Screening of Class I Integron in Salmonella Isolates Recovered from Retail Raw Chicken Carcasses in China

Class 1 Integrons and the Antibiotic Resistance Profile of Salmonella Infantis Strains from Broiler Chickens

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