Molecular Clock as Insight to Estimate the Evolutionary History and Times of Divergence for 10 Nominal <i>Astyanax</i> Species (Characiformes, Characidae): An Evolutionary Approach in Species with 2n = 36, 46, 48, and 50 Chromosomes

Piscor, Diovani; Pozzobon, Allan Pierre Bonetti; Fernandes, Carlos Alexandre; Centofante, Liano; Parise-Maltempi, Patrícia Pasquali

doi:10.1089/zeb.2018.1647

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…We first used the A. mexicanus transcriptome as reference to estimate 1B/0B coverage ratio for the contigs claimed by authors as being in the B chromosome of A. correntinus (61 and 26 contigs, the sequence of which we downloaded from EBI and NCBI, respectively). In different mappings, we expressed the results as “number of reads mapped” (as done in [ 1 ]) or in “number of copies per haploid genome” (as done in [ 2 ]). According to [ 1 ], all 61 and 26 contigs passed the 1.5 coverage ratio threshold.…”

Section: Resultsmentioning

confidence: 99%

Irreproducible results and unsupported conclusions in Ahmad et al. [BMC genomics (2020) 21:656]

Ruiz-Ruano,

Camacho

2023

BMC Genomics

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Section: Resultsmentioning

confidence: 99%

Irreproducible results and unsupported conclusions in Ahmad et al. [BMC genomics (2020) 21:656]

Ruiz-Ruano,

Camacho

2023

BMC Genomics

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“…In this genera the karyotype variation is accompanied by high intra/interspecific and interpopulational divergence of diploid number (Pazza et al, 2018), due to events of fusion and centric fission, often accompanied by inversions and translocations, common in Astyanax spp. (Fernandes, Martins-Santos, 2005;Pazza et al, 2008;Piscor et al, 2019; among others). The two populations of B. aff.…”

Section: Discussionmentioning

confidence: 98%

Cytogenetic markers as tools in delimiting species of the highly diverse Neotropical fish Bryconamericus (Characiformes: Characidae)

et al. 2019

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Bryconamericus is a highly diverse group of characid fishes, being cytogenetic a valuable tool for the delimitation of species. Bryconamericus aff. iheringii (Upper Uruguay/Lower Paraná), B. coeruleus (Upper Paraná), B. cf. ecai e B. cf. eigenmanni (Upper Uruguay) were studied cytogenetically, and presented 2n=52 chromosomes, with interpopulational/interspecific variation of karyotype and fundamental number. Heterochromatin was evidenced in pericentromeric, telomeric and interstitial regions, and it was shown to be an important cytogenetic marker. Single nucleolar organizing regions (NORs) were found in B. cf. eigenmanni, B. cf. ecai and B. aff. iheringii (Lower Paraná), and multiple in B. aff. iheringii (Upper Uruguay) and B. coeruleus, with occurrence of two patterns for the first species, and three for the second. The 5S/18S rDNA-FISH confirmed the location of the NORs and showed single 5S rDNA cistrons only in B. aff. iheringii (Lower Paraná), evidencing the dispersion of both genes, often co-located, in the karyotype of the others species. The data of this work contribute for the delimitation of the species of the genus. Co-localization of ribosomal genes may represent a plesiomorphic condition for the group, and their dispersion suggest the occurrence of duplication, pseudogeneization and transposition events mediated by mobile genetic elements.

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“…In addition to the Charsat01-52 and telomeric sequence, we found another eight satDNA families present on the four satellitomes. These satDNAs were maintained from 11.2 mya, when A. lacustris diverged from Psalidodon ( Piscor et al, 2019 ). The maintenance of satDNA families across different species can occur through the biological function of determinate satDNA ( Fry and Salser, 1977 ) or independence of natural selection ( Stephan, 1986 ; Stephan, 1987 ; Walsh, 1987 ; Stephan, 1989 ; Harding et al, 1992 ).…”

Section: Discussionmentioning

confidence: 99%

Revealing the Satellite DNA History in Psalidodon and Astyanax Characid Fish by Comparative Satellitomics

Goes¹,

Santos²,

Aguiar³

et al. 2022

Front. Genet.

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Eukaryotic genomes are usually enriched in repetitive DNA sequences, which can be classified as dispersed or tandemly repeated elements. Satellite DNAs are noncoding monomeric sequences organized in a head-to-tail fashion that are generally located on the subtelomeric and/or pericentromeric heterochromatin. In general, a single species incorporates a diverse group of satellite DNA families, which collection is called satellitome. Here, we characterized three new satellitomes from distinct characid fish (Psalidodon fasciatus, P. bockmanni, and Astyanax lacustris) using a combination of genomic, cytogenetic, and bioinformatic protocols. We also compared our data with the available satellitome of P. paranae. We described 57 satellite DNA (satDNA) families of P. fasciatus (80 variants), 50 of P. bockmanni (77 variants), and 33 of A. lacustris (54 variants). Our analyses demonstrated that several sequences were shared among the analyzed species, while some were restricted to two or three species. In total, we isolated 104 distinctive satDNA families present in the four species, of which 10 were shared among all four. Chromosome mapping revealed that the clustered satDNA was mainly located in the subtelomeric and pericentromeric areas. Although all Psalidodon species demonstrated the same pattern of clusterization of satDNA, the number of clusters per genome was variable, indicating a high dynamism of these sequences. In addition, our results expand the knowledge of the As51 satellite DNA family, revealing that P. bockmanni and P. paranae exhibited an abundant variant of 39 bp, while P. fasciatus showed a variant of 43 bp. The majority of satDNAs in the satellitomes analyzed here presented a common library repetitive sequence in Psalidodon and Astyanax, with abundance variations in each species, as expected for closely related groups. In addition, we concluded that the most abundant satDNA in Psalidodon (As51) passed through a diversification process in this group, resulting in new variants exclusive of Psalidodon.

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Molecular Clock as Insight to Estimate the Evolutionary History and Times of Divergence for 10 Nominal Astyanax Species (Characiformes, Characidae): An Evolutionary Approach in Species with 2n = 36, 46, 48, and 50 Chromosomes

Cited by 5 publications

References 48 publications

Irreproducible results and unsupported conclusions in Ahmad et al. [BMC genomics (2020) 21:656]

Irreproducible results and unsupported conclusions in Ahmad et al. [BMC genomics (2020) 21:656]

Cytogenetic markers as tools in delimiting species of the highly diverse Neotropical fish Bryconamericus (Characiformes: Characidae)

Revealing the Satellite DNA History in Psalidodon and Astyanax Characid Fish by Comparative Satellitomics

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