Molecular Choreography and Structure of Ca2+ Release-Activated Ca2+ (CRAC) and KCa2+ Channels and Their Relevance in Disease with Special Focus on Cancer

Tiffner, Adéla; Derler, Isabella

doi:10.3390/membranes10120425

Cited by 10 publications

(7 citation statements)

References 462 publications

(868 reference statements)

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“…As shown in Figure 3 , in the model, customer experience is the prevariable of brand loyalty. Brand loyalty is divided into two dimensions: behavior and attitude loyalty, and customer consumption (CS) is introduced to expand this framework [ 11 , 12 ]. Measured by consumers' share of purchases of a particular brand relative to overall usage or spending on products in the same category.…”

Section: Model Constructionmentioning

confidence: 99%

Analyzing the Relationship between Hotel Brand Image, Service Quality, Experience Marketing, and Customer Satisfaction under the Environment of Social Network

2022

Journal of Environmental and Public Health

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Based on the development background of the social network environment, higher requirements are put forward for the development and transformation of hotels in the new era. As a representative industry in the service industry, the service quality and experience provided by the hotel can meet the feelings and needs of customers. According to the data of the hotel industry in previous years, it can be found that from 2015 to 2018, the average revenue of hotel rooms available for rent (RevPAR) decreased by 7.7%, 5.3%, and 7.7%, respectively; The occupancy rate dropped to the lowest point at the end of 2018 and then began to rise gradually. In addition, while the economy recovers, the tourism industry has driven the hotel industry. RevPAR has increased by—1.4% and 3.5% year-on-year, and the occupancy rate has increased by 3.6% and 2.0% year-on-year. In 2018, China’s star hotels generally showed an upward trend. This also shows the attraction of hotel brand logo to customers. By studying the factors such as brand image and service quality, we can establish the viscosity with customers. This paper explores the relationship between various elements and customers and puts forward effective suggestions in order to further improve the service level of the hotel.

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Section: Model Constructionmentioning

confidence: 99%

Analyzing the Relationship between Hotel Brand Image, Service Quality, Experience Marketing, and Customer Satisfaction under the Environment of Social Network

2022

Journal of Environmental and Public Health

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“…Several reports have already provided evidence of an interplay of Ca 2+ and Ca 2+ regulated potassium (K + ) ion channels such as Ca V 2.3 with BK channels [ 9 ], Orai1 with the ether a-gogo K + channel hEag1 [ 10 ] or BK channels [ 11 , 12 ], Orai1 [ 13 ], or TRPV6 [ 14 ] with the small conductance Ca 2+ -activated activated K + channels SK4 and Orai1 with its homolog SK3 [ 13 , 15 , 16 , 17 , 18 , 19 ]. The interplay of the Ca 2+ ion channel Orai1 with the Ca 2+ -activated K + ion channel SK3 is currently best studied in breast cancer cells [ 16 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Orai1 Boosts SK3 Channel Activation

Tiffner

Hopl

Schober

et al. 2021

Cancers

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The interplay of SK3, a Ca2+ sensitive K+ ion channel, with Orai1, a Ca2+ ion channel, has been reported to increase cytosolic Ca2+ levels, thereby triggering proliferation of breast and colon cancer cells, although a molecular mechanism has remained elusive to date. We show in the current study, via heterologous protein expression, that Orai1 can enhance SK3 K+ currents, in addition to constitutively bound calmodulin (CaM). At low cytosolic Ca2+ levels that decrease SK3 K+ permeation, co-expressed Orai1 potentiates SK3 currents. This positive feedback mechanism of SK3 and Orai1 is enabled by their close co-localization. Remarkably, we discovered that loss of SK3 channel activity due to overexpressed CaM mutants could be restored by Orai1, likely via its interplay with the SK3–CaM binding site. Mapping for interaction sites within Orai1, we identified that the cytosolic strands and pore residues are critical for a functional communication with SK3. Moreover, STIM1 has a bimodal role in SK3–Orai1 regulation. Under physiological ionic conditions, STIM1 is able to impede SK3–Orai1 interplay by significantly decreasing their co-localization. Forced STIM1–Orai1 activity and associated Ca2+ influx promote SK3 K+ currents. The dynamic regulation of Orai1 to boost endogenous SK3 channels was also determined in the human prostate cancer cell line LNCaP.

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“…We further discovered that one critical determinant for this co-regulation is the CaM binding site in SK3, because Orai1 overexpression decreased the interaction of SK3 with CaM as well as the CaM MUT s. Moreover, SK3 K + currents abolished by the CaM MUT were partially restored by co-expression of Orai1. At the level of Orai1, an intact pore and both cytosolic strands, except the first 26 aa, are indispensable for the co-regulation of SK3 and Orai1 [112,301].…”

Section: Lipid-mediated Modulation Of the Co-regulation Of Crac Chann...mentioning

confidence: 99%

The Role of Lipids in CRAC Channel Function

2022

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The composition and dynamics of the lipid membrane define the physical properties of the bilayer and consequently affect the function of the incorporated membrane transporters, which also applies for the prominent Ca2+ release-activated Ca2+ ion channel (CRAC). This channel is activated by receptor-induced Ca2+ store depletion of the endoplasmic reticulum (ER) and consists of two transmembrane proteins, STIM1 and Orai1. STIM1 is anchored in the ER membrane and senses changes in the ER luminal Ca2+ concentration. Orai1 is the Ca2+-selective, pore-forming CRAC channel component located in the plasma membrane (PM). Ca2+ store-depletion of the ER triggers activation of STIM1 proteins, which subsequently leads to a conformational change and oligomerization of STIM1 and its coupling to as well as activation of Orai1 channels at the ER-PM contact sites. Although STIM1 and Orai1 are sufficient for CRAC channel activation, their efficient activation and deactivation is fine-tuned by a variety of lipids and lipid- and/or ER-PM junction-dependent accessory proteins. The underlying mechanisms for lipid-mediated CRAC channel modulation as well as the still open questions, are presented in this review.

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Molecular Choreography and Structure of Ca2+ Release-Activated Ca2+ (CRAC) and KCa2+ Channels and Their Relevance in Disease with Special Focus on Cancer

Cited by 10 publications

References 462 publications

Analyzing the Relationship between Hotel Brand Image, Service Quality, Experience Marketing, and Customer Satisfaction under the Environment of Social Network

Analyzing the Relationship between Hotel Brand Image, Service Quality, Experience Marketing, and Customer Satisfaction under the Environment of Social Network

Orai1 Boosts SK3 Channel Activation

The Role of Lipids in CRAC Channel Function

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