Molecular characterization, transcriptional profiling, and antibacterial potential of G-type lysozyme from seahorse ( Hippocampus abdominalis )

Ko, Jung Ho; Wan, Qiang; Bathige, S.D.N.K.; Lee, Jehee

doi:10.1016/j.fsi.2016.10.014

Cited by 25 publications

(11 citation statements)

References 44 publications

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“…The first was most abundant in the liver and the second was predominantly expressed in the intestine and weakly expressed in the muscle [ 38 ]. A g-type lysozyme was identified in 2016 [ 39 ] in seahorses ( Hippocampus abdominalis ), with the highest expression in the kidney and the least expression in the liver.…”

Section: Sourcesmentioning

confidence: 99%

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Ferraboschi

Ciceri

Grisenti³

2021

Antibiotics

174

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Lysozyme is a ~14 kDa protein present in many mucosal secretions (tears, saliva, and mucus) and tissues of animals and plants, and plays an important role in the innate immunity, providing protection against bacteria, viruses, and fungi. Three main different types of lysozymes are known: the c-type (chicken or conventional type), the g-type (goose type), and the i-type (invertebrate type). It has long been the subject of several applications due to its antimicrobial properties. The problem of antibiotic resistance has stimulated the search for new molecules or new applications of known compounds. The use of lysozyme as an alternative antibiotic is the subject of this review, which covers the results published over the past two decades. This review is focused on the applications of lysozyme in medicine, (the treatment of infectious diseases, wound healing, and anti-biofilm), veterinary, feed, food preservation, and crop protection. It is available from a wide range of sources, in addition to the well-known chicken egg white, and its synergism with other compounds, endowed with antimicrobial activity, are also summarized. An overview of the modified lysozyme applications is provided in the form of tables.

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Section: Sourcesmentioning

confidence: 99%

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Ferraboschi

Ciceri

Grisenti³

2021

Antibiotics

174

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“…As another broad‐spectrum bactericidal peptide, regIIIgamma inhibits deleterious bacteria and regulates the spatial relationships between microbiota and host to promote host‐bacterial mutualism 106 . ShLvsG is an ortholog of goose‐type lysozyme, characterized from seahorse ( Hippocampus abdominalis ), 107 and lytic activities of this AMP indicate it is an antimicrobial effector of the innate immune system by hydrolyzing the wall of bacterial cell 108,109 . Although these four AMPs have not been identified or isolated from fish species, we determined that there were four potential corresponding AMPs from the catfish proteome (accession numbers: XP_017313286.1, XP_017328327.1, XP_017350515.1 and XP_017328787.1, Supplement S2).…”

Section: Categories and Properties Of Catfish Ampsmentioning

confidence: 99%

Genome‐wide identification of catfish antimicrobial peptides: A new perspective to enhance fish disease resistance

Wang

Dunham

2022

Reviews in Aquaculture

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Antimicrobial peptides (AMPs), also known as host defence peptides, are evolutionarily ancient defensive weapons that can combat infections caused by pathogens. Fish‐derived AMPs have shown microbicidal properties, host immunomodulatory responses, and are induced by a variety of factors, especially pathogenic infection and abiotic stress, which may activate downstream signalling pathways to initiate the expression of antimicrobial peptide genes. Large‐scale genome sequencing has been applied in many aquatic animals, providing databases for the systematic identification of putative AMPs in these species. Recently, the whole genome and the proteome of channel catfish (Ictalurus punctatus) were released; by taking advantage of the antimicrobial peptide database and combining the proteome of catfish, we established a bioinformatic analysis pipeline to identify catfish AMP repertoire. This review introduces an effective method for the identification and development of putative AMPs based on protein datasets, and summarizes the structural properties, immunomodulation and molecular responses in vivo of catfish AMPs. In addition, the evaluation of the antibacterial activity of synthetic or isolated AMPs in vitro, and the applications of AMPs in fish via genetic engineering and clustered regularly interspaced short palindromic repeats associated proteins system (CRISPR/Cas9) were presented, which supports antimicrobial research and aquaculture therapy. The summary and comparison of these AMPs will enhance our understanding and their cross‐species applications. Combined with current genetic engineering and genome editing techniques, it is possible to promote the development of immune processes to protect valuable aquatic animals from infectious diseases.

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“…In addition, HKPLP appeared to have a minor inhibitory effect on fungi. Ko et al (2016) identified a goose-type lysozyme (ShLysG) from Hippocampus abdominalis Lesson that had a certain inhibitory effect against gram-positive/negative bacteria and a potential immune-protecting effect against pathogens. Bacterial pathogen factors are one of the important factors affecting the survival of marine organisms.…”

Section: Water Extractionmentioning

confidence: 99%

Advances in the Global Distribution, Chemical Constituents, and Pharmacology of Hippocampus, a Traditional Marine Chinese Medicine

Cui

Zhao

et al. 2021

Front. Mar. Sci.

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Hippocampus is an important traditional marine Chinese medicinal resource that has been used to warm and tonify kidney yang in the clinic for a long time in China. Modern pharmacological studies show that its active ingredients display a wide range of pharmacological activities associated with the kidney, such as anti-inflammation, antioxidation, antitumor, and neuroprotective effects. Herein, we systematically summarize and analyze the research progress on the resource distribution, active ingredients, pharmacological activities, and clinical application of Hippocampus. First, the species and worldwide distribution of Hippocampus were assessed to clarify the existing resources, and the results showed that 44 species of Hippocampus have been found in 159 countries and regions worldwide. Then, based on the analysis of 16 kinds of active ingredients and extraction methods, the relationship between the ingredient extraction and pharmacological activities of Hippocampus was revealed. This review may provide a foundation for further research on the potential active ingredients and mechanisms of Hippocampus. In addition, the research status of traditional prescriptions containing Hippocampus was evaluated. The results implied that research on Hippocampus is still in its infancy, and the mechanism and material basis of its efficacy have not been clarified. This paper should provide directions for further studies on Hippocampus.

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Molecular characterization, transcriptional profiling, and antibacterial potential of G-type lysozyme from seahorse ( Hippocampus abdominalis )

Cited by 25 publications

References 44 publications

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Genome‐wide identification of catfish antimicrobial peptides: A new perspective to enhance fish disease resistance

Advances in the Global Distribution, Chemical Constituents, and Pharmacology of Hippocampus, a Traditional Marine Chinese Medicine

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