Molecular characterization, tissue expression of acyl-CoA ∆9-desaturase-like gene, and effects of dietary lipid levels on its expression in the hepatopancreas of the Chinese mitten crab (Eriocheir sinensis)

Guo, Zengjun; Yang, Ziyi; Cheng, Yongxu; Ji, Lianyuan; Que, You-qing; Liu, Zhiwei; Zeng, Qing‐Yin

doi:10.1016/j.aquaculture.2013.03.033

Cited by 22 publications

(10 citation statements)

References 32 publications

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“…Under physiological conditions, the desaturase gene family members are evolutionarily conserved and they play an important role in the regulation of fatty‐acid metabolism (Guo et al ., ). In the present study, the increasing graded levels of dietary SO up‐regulated the mRNA levels of Δ6 fad , which was well correlated with the increase in ARA content of the viscera.…”

Section: Discussionmentioning

confidence: 97%

Growth performance, fatty‐acid composition, lipid deposition and hepatic‐lipid metabolism‐related gene expression in juvenile pond loach Misgurnus anguillicaudatus fed diets with different dietary soybean oil levels

Jia

Liang

et al. 2017

Journal of Fish Biology

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A 10 week feeding trial was conducted to evaluate the effects of different dietary soybean oil (SO) levels on growth performance, fatty-acid composition and lipid deposition in viscera, histology and histochemistry of liver, intestine and hepatic-lipid metabolism-related gene expressions in pond loach Misgurnus anguillicaudatus juveniles. Misgurnus anguillicaudatus (mean ± s.d. mass 0·40 ± 0·01 g) were fed five experimental diets containing SO at different concentrations: 0, 20, 32, 56 and 100% SO and a diet containing 100% fish oil (100% FO). The mass gains and specific growth rates of M. anguillicaudatus fed 20% SO and 100% FO diets were significantly higher than those of the other groups (P < 0·05). The lipid content of viscera and the amount of cytoplasmic vacuolation in the liver increased with incremental dietary SO level. Meanwhile, increasing dietary SO levels up-regulated the messenger (m)RNA levels of lipogenic genes (such as Δ6fad, scd, ppar , fas and srebp-1) and down-regulated the mRNA levels of the lipolytic genes (such as ppar , cpt1, atgl and hsl) in the liver. The percentage of 20:4n-6 significantly (P < 0·05) increased with increasing dietary SO level, which might be correlated with the up-regulation of the mRNA level of Δ6fad. The highest levels of dietary SO, however, had a negative effect on growth performance, lipid deposition of viscera and histology and histochemstry of liver and intestine. The increased lipid accumulation induced by incremental dietary SO level probably occurred through different strategies for lipid metabolism as a result of competition between lipolysis and lipogenesis and between export and import of lipids in this species.

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Section: Discussionmentioning

confidence: 97%

Growth performance, fatty‐acid composition, lipid deposition and hepatic‐lipid metabolism‐related gene expression in juvenile pond loach Misgurnus anguillicaudatus fed diets with different dietary soybean oil levels

Jia

Liang

et al. 2017

Journal of Fish Biology

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“…Guo and colleagues were the first to isolate fatty acyl Δ9-desaturase from E . sinensis [ 55 ], and it has been characterized in BL21(DE3)pLysS, fatty acyl Δ9-desaturase in E . sinensis had an activity in the desaturation of C18:0 [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)

et al. 2017

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Fish oil supplies worldwide have declined sharply over the years. To reduce the use of fish oil in aquaculture, many studies have explored the effects of fish oil substitutions on aquatic animals. To illustrate the effects of dietary lipids on Chinese mitten crab and to improve the use of vegetable oils in the diet of the crabs, 60 male juvenile Chinese mitten crabs were fed one of five diets for 116 days: fish oil (FO), soybean oil (SO), linseed oil (LO), FO + SO (1:1, FSO), and FO + LO (1:1, FLO). Changes in the crab hepatopancreas transcriptome were analyzed using RNA sequencing. There were a total 55,167 unigenes obtained from the transcriptome, of which the expression of 3030 was significantly altered in the FLO vs. FO groups, but the expression of only 412 unigenes was altered in the FSO vs. FO groups. The diets significantly altered the expression of many enzymes involved in lipid metabolism, such as pancreatic lipase, long-chain acyl-CoA synthetases, carnitine palmitoyltransferase I, acetyl-CoA carboxylase, fatty acid synthase, and fatty acyl Δ9-desaturase. The dietary lipids also affected the Toll-like receptor and Janus activated kinase-signal transducers and activators of transcription signaling pathways. Our results indicate that substituting fish oil with vegetable oils in the diet of Chinese mitten crabs might decrease the digestion and absorption of dietary lipids, fatty acids biosynthesis, and immunologic viral defense, and increase β-oxidation by altering the expression of the relevant genes. Our results lay the foundation for further understanding of lipid nutrition in Chinese mitten crab.

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“…SCD plays an important role in fatty acid metabolism (Guo et al., ) by catalysing the conversion of saturated fatty acids to n‐9 monounsaturated fatty acids (Eigenheer et al., ). Additionally, decreased SCD activity is associated with decreased fatty acid synthesis (Bjermo & Risérus, ; Sampath, Miyazaki, Dobrzyn, & Ntambi, ).…”

Section: Discussionmentioning

confidence: 99%

An evaluation of increasing linolenic acid level in the diet ofMacrobrachium nipponense: Lipid deposition, fatty acid composition and expression of lipid metabolism-related genes

et al. 2017

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To determine the effects of linolenic acid (LNA, in oriental river prawn (Macrobrachium nipponense), an 8-week feeding experiment was conducted using six isonitrogenous and isoenergetic semi-purified diets containing 0.07 g/kg (control), 7.3 g/kg, 16.6 g/kg, 20.2 g/kg, 27.3 g/kg and 36.3 g/kg LNA. The hepatopancreas lipid content decreased significantly when dietary LNA content was >20.2 g/kg. Fatty acid analysis revealed that the percentage of 18:3n-3 in the hepatopancreas significantly increased with increasing dietary LNA levels, while 20:5n-3, 22:5n-3 and 22:6n-3 levels in the hepatopancreas decreased in a curvilinear manner as dietary LNA increased. Additionally, qRT-PCR results revealed that hepatopancreas mRNA expression of acetyl-CoA carboxylase (ACC) decreased with increasing dietary LNA, while the greatest carnitine palmitoyl transferase-1(CPT1) mRNA expression was observed in the 2.73 g/kg and 36.3 g/kg groups. Furthermore, hepatopancreas mRNA expression of acyl-CoA delta-9 desaturase (SCD) and fatty acyl elongase 6(elovl6) was downregulated when prawns fed the diets containing >20.2 g/kg LNA. These results indicate that dietary 18:3n-3 could decrease lipid deposition through increased fatty acid β-oxidation and modulated fatty acid synthesis, and alter fatty acid composition by regulating fatty acyl elongase and fatty acyl desaturase mRNA expression in the M. nipponense. K E Y W O R D S fatty acid composition, linolenic acid, lipid deposition, Macrobrachium nipponense, mRNA expression

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Molecular characterization, tissue expression of acyl-CoA ∆9-desaturase-like gene, and effects of dietary lipid levels on its expression in the hepatopancreas of the Chinese mitten crab (Eriocheir sinensis)

Cited by 22 publications

References 32 publications

Growth performance, fatty‐acid composition, lipid deposition and hepatic‐lipid metabolism‐related gene expression in juvenile pond loach Misgurnus anguillicaudatus fed diets with different dietary soybean oil levels

Growth performance, fatty‐acid composition, lipid deposition and hepatic‐lipid metabolism‐related gene expression in juvenile pond loach Misgurnus anguillicaudatus fed diets with different dietary soybean oil levels

Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)

An evaluation of increasing linolenic acid level in the diet ofMacrobrachium nipponense: Lipid deposition, fatty acid composition and expression of lipid metabolism-related genes

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