Molecular Characterization, Tissue Distribution, Subcellular Localization and Actin-Sequestering Function of a Thymosin Protein from Silkworm

Zhang, Wenping; Zhang, Changrong; Lv, Zhengbing; Fang, Dailing; Wang, Dan; Nie, Zhou; Yu, Wengong; Lan, Hanglian; Jiang, Cheng; Zhang, Yaozhou

doi:10.1371/journal.pone.0031040

Cited by 7 publications

(11 citation statements)

References 44 publications

(47 reference statements)

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“…A Nikon Confocal Microscope and EZ‐C1 software were used to analyze the treated cells. Cy3‐labeled goat antirabbit IgG emitted red fluorescence when stimulated with light at 550 nm, and DAPI‐stained nuclei emitted blue fluorescence when stimulated with light at 353 nm (Zhang et al., ). The result showed that BmATAT1 is located in the cytoplasm and cell nucleus (Figure ).…”

Section: Resultsmentioning

confidence: 99%

Cloning, expression profiling, and acetylation identification of alpha‐tubulin N‐acetyltransferase 1 from Bombyx mori

Zhou

Cheng

et al. 2018

Arch Insect Biochem Physiol

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Alpha-tubulin N-acetyltransferase 1 (ATAT1) is an acetyltransferase specific to α-tubulin and performs important functions in many cellular processes. Bombyx mori is an economic insect and also known as a model lepidoptera insect. In this study, we cloned a B. mori ATAT1 gene (BmATAT1) (Gen Bank accession number: XP_004932777.1). BmATAT1 contained an open reading frame (ORF) of 1,065 bp encoding 355 amino acids (aa). Expression profiling of BmATAT1 protein showed that the expression levels of BmATAT1 at different developmental stages and different tissues in fifth-instar larvae differ. BmATAT1 was highly expressed at the egg stage and in the head of the fifth-instar larvae. Subcellular localization showed that BmATAT1 was distributed in the cytoplasm and nucleus. Furthermore, BmATAT1 may lead to time-dependent induction of cell cycle arrest in the G2/M phase by flow cytometry analysis. Interestingly, using site-specific mutation, immunoprecipitation, and Western blotting, we further found a BmATAT1 acetylated site at K156, suggesting that this acetyltransferase could be regulated by acetylation itself.

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Section: Resultsmentioning

confidence: 99%

Cloning, expression profiling, and acetylation identification of alpha‐tubulin N‐acetyltransferase 1 from Bombyx mori

Zhou

Cheng

et al. 2018

Arch Insect Biochem Physiol

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“…In bulls, TMSB10 protein was primarily detected in the head and tail area of testicular and ejaculated sperms, but the expression was limited to the equatorial segment of sperm during epididymal development (Howes et al, ). A homologous thymosin β protein in silkworm was highly expressed in the ovarian cells at the fifth‐instar larval period, however the expression of this protein was detected lower in the testis (Zhang et al, ). These studies suggested that the expression and functions of the thymosin family members varies slightly among species.…”

Section: Discussionmentioning

confidence: 99%

Comparative expression and regulation of TMSB4X in male reproductive tissues of rats and chickens

et al. 2013

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Thymosin β4 (TMSB4X) belongs to a class of highly conserved small proteins that are present in a high abundance in immune tissues, where it participates in various biological activities, including anti-inflammation, wound healing, apoptosis, and cell survival. However, little is known about the expression and regulation of TMSB4X in reproductive tissues. The aim of this study was to examine the expression of rat Tmsb4x and chicken TMSB4X genes during testicular and epididymal development. Rat Tmsb4x was strongly detected in the spermatogonia and spermatocytes of testis at early postnatal development and transmitted to Leydig cells at sexual maturation. Also, rat Tmsb4x was detected at an increased level in the epididymis during postnatal development. When compared to the rat, expression of the chicken TMSB4X gene was low in the testis and epididymal region, and the mRNA localization was also unexpected. Three experiments were performed to examine the regulation of rat Tmsb4x in the epididymis: after elimination of Leydig cells using ethylene dimethane sulfonate (EDS); after destruction of the testis by cryptorchidism; and after castration. EDS-treated and castrated rats were injected with testosterone propionate. The expression of Tmsb4x was significantly reduced in the epididymis of EDS-treated, and castrated rats. In contrast, Tmsb4x was significantly enhanced in the epididymis after testosterone treatment. The expression of rat Tmsb4x was also regulated in the epididymis after cryptorchidism. Collectively, the expression of Tmsb4x was strongly detected in the testis and epididymis of rats, and was highly regulated in the epididymis by testosterone.

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“…Real-time fluorescence quantitative RT-PCR primers of B. mori 18s rRNA have been reported previously (Zhang et al, 2012). The primers and their annealing temperatures are listed in Table 1.…”

Section: Cloning and Real-time Fluorescence Quantitative Rt-pcr Primementioning

confidence: 99%

Molecular characterization, tissue distribution, and immune reaction expression of karyopherins in the domestic silkworm (Bombyx mori)

Wang

Qian

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Karyopherins, including alpha and beta types, are transport proteins in the eukaryotic cell that carry cargoes across nuclear pore complexes into or out of the nucleus. In this study, full open reading frames of one beta and three alpha types of karyopherin were cloned from cDNA of the domestic silkworm (Bombyx mori). The one beta and three alpha types' open reading frames were 2661, 1563, 1515, and 1551 base pairs long, respectively, and coded 886, 520, 504, and 516 amino acids, respectively. The alphas all had one importin-beta-binding (IBB) domain, and eight, four, or seven armadillo/beta-catenin-like repeats. The beta had 19 HEAT repeat domains, which constructed one importin-beta-N-terminal domain and one IBB domain. The recombinant proteins were expressed in Escherichia coli cells. The molecular weight of the beta type was approximately 100 kDa, and the alphas weighed approximately 60 kDa. Phylogenic tree construction revealed that the alphas could be classified into three known karyopherin-alpha subfamilies. We detected mRNA of the four karyopherins in normal 3rd day of 5th instar larvae, and in larvae injected with Grampositive bacteria, Gram-negative bacteria, viruses, and fungi using real- time fluorescence quantitative reverse transcriptase-polymerase chain reaction, and found that the four karyopherins were widely distributed, but their expression levels were related to tissues type, the microbe injected, and the time point.

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Molecular Characterization, Tissue Distribution, Subcellular Localization and Actin-Sequestering Function of a Thymosin Protein from Silkworm

Cited by 7 publications

References 44 publications

Cloning, expression profiling, and acetylation identification of alpha‐tubulin N‐acetyltransferase 1 from Bombyx mori

Cloning, expression profiling, and acetylation identification of alpha‐tubulin N‐acetyltransferase 1 from Bombyx mori

Comparative expression and regulation of TMSB4X in male reproductive tissues of rats and chickens

Molecular characterization, tissue distribution, and immune reaction expression of karyopherins in the domestic silkworm (Bombyx mori)

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