Molecular characterization, tissue distribution, and ultrastructural localization of adipokinetic hormones in the CNS of the firebug Pyrrhocoris apterus (Heteroptera, Insecta)

Kodrı́k, Dalibor; Stašková, Tereza; Jedličková, Veronika; Weyda, F.; Závodská, Radka; Pflegerová, Jitka

doi:10.1016/j.ygcen.2014.10.014

Cited by 15 publications

(5 citation statements)

References 61 publications

(89 reference statements)

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“…Unexpectedly, a minor expression was also detected in other tissues. AKH expression outside of CC was previously reported in fat bodies of A. mellifera workers (Wang et al, 2012) and brain tissues (i.e., without CC-CA) of the red firebug, P. apterus , where the translated neuropeptide was also localized and quantified (Kodrík et al, 2015). However, the significance of these observations is not clear yet.…”

Section: Resultsmentioning

confidence: 77%

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Jedlička

Ernst

Votavová³

et al. 2016

Front. Physiol.

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Understanding the social evolution leading to insect eusociality requires, among other, a detailed insight into endocrine regulatory mechanisms that have been co-opted from solitary ancestors to play new roles in the complex life histories of eusocial species. Bumblebees represent well-suited models of a relatively primitive social organization standing on the mid-way to highly advanced eusociality and their queens undergo both, a solitary and a social phase, separated by winter diapause. In the present paper, we characterize the gene expression levels of major endocrine regulatory pathways across tissues, sexes, and life-stages of the buff-tailed bumblebee, Bombus terrestris, with special emphasis on critical stages of the queen's transition from solitary to social life. We focused on fundamental genes of three pathways: (1) Forkhead box protein O and insulin/insulin-like signaling, (2) Juvenile hormone (JH) signaling, and (3) Adipokinetic hormone signaling. Virgin queens were distinguished by higher expression of forkhead box protein O and downregulated insulin-like peptides and JH signaling, indicated by low expression of methyl farnesoate epoxidase (MFE) and transcription factor Krüppel homolog 1 (Kr-h1). Diapausing queens showed the expected downregulation of JH signaling in terms of low MFE and vitellogenin (Vg) expressions, but an unexpectedly high expression of Kr-h1. By contrast, reproducing queens revealed an upregulation of MFE and Vg together with insulin signaling. Surprisingly, the insulin growth factor 1 (IGF-1) turned out to be a queen-specific hormone. Workers exhibited an expression pattern of MFE and Vg similar to that of reproducing queens. Males were characterized by high Kr-h1 expression and low Vg level. The tissue comparison unveiled an unexpected resemblance between the fat body and hypopharyngeal glands across all investigated genes, sexes, and life stages.

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Section: Resultsmentioning

confidence: 77%

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Jedlička

Ernst

Votavová³

et al. 2016

Front. Physiol.

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“…Expression of AKHRs in the brain occurs in other insects (e.g. Periplaneta americana and Rhodnius prolixus) and might be linked to stress situations (Kodrík et al, 2015;Wicher et al, 2006;Zandawala et al, 2015). In A. aegypti, akhr transcripts are expressed in the thorax, but not in the head or brain Fig.…”

Section: Discussionmentioning

confidence: 99%

Characterization and pharmacological analysis of two adipokinetic hormone receptor variants of the tsetse fly, Glossina morsitans morsitans

Caers

Janssen

Rompay

et al. 2016

Insect Biochemistry and Molecular Biology

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“…A mechanism explaining a role of these stressors in triggering the adipokinetic response is not yet known. However, it seems logical that at least in the case of injury, the nervous system might be involved [ 54 ]; nevertheless, in the absence of direct information, the possible involvement of other players (e.g., biogenic amines) in the cascade remains a speculation.…”

Section: Adipokinetic Hormones—main Stress Hormones In Insectsmentioning

confidence: 99%

Hormonal Regulation of Response to Oxidative Stress in Insects—An Update

Kodrı́k

Bednářová

Zemanová

et al. 2015

IJMS

Self Cite

121

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Insects, like other organisms, must deal with a wide variety of potentially challenging environmental factors during the course of their life. An important example of such a challenge is the phenomenon of oxidative stress. This review summarizes the current knowledge on the role of adipokinetic hormones (AKH) as principal stress responsive hormones in insects involved in activation of anti-oxidative stress response pathways. Emphasis is placed on an analysis of oxidative stress experimentally induced by various stressors and monitored by suitable biomarkers, and on detailed characterization of AKH’s role in the anti-stress reactions. These reactions are characterized by a significant increase of AKH levels in the insect body, and by effective reversal of the markers—disturbed by the stressors—after co-application of the stressor with AKH. A plausible mechanism of AKH action in the anti-oxidative stress response is discussed as well: this probably involves simultaneous employment of both protein kinase C and cyclic adenosine 3′,5′-monophosphate pathways in the presence of extra and intra-cellular Ca2+ stores, with the possible involvement of the FoxO transcription factors. The role of other insect hormones in the anti-oxidative defense reactions is also discussed.

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Molecular characterization, tissue distribution, and ultrastructural localization of adipokinetic hormones in the CNS of the firebug Pyrrhocoris apterus (Heteroptera, Insecta)

Cited by 15 publications

References 61 publications

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Characterization and pharmacological analysis of two adipokinetic hormone receptor variants of the tsetse fly, Glossina morsitans morsitans

Hormonal Regulation of Response to Oxidative Stress in Insects—An Update

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