Molecular Characterization, Tissue Distribution and Differential Nutritional Regulation of Three n-3 LC-PUFA Biosynthesis-Related Genes in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

Wu, Qingjun; Zheng, Zhi; Wang, Chuijin; Wang, Yao; Sun, Yuejia; Gao, Yujie

doi:10.3390/ani12030234

Cited by 3 publications

(5 citation statements)

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“…In this study, the elovl8 gene of the yellow catfish was identified and characterized; it contained a 795-bp-long ORF that encodes a protein of 264 amino acids. Similar results were also observed in rabbitfish (Siganus canaliculatus) (Li et al, 2020) and hybrid grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂) (Wu et al, 2022), that the elovl8 genes are conserved among different fishes.…”

Section: Discussionsupporting

confidence: 81%

“…Previous studies have shown that diets full of HUFAs could inhibit the transcriptional expression in rabbitfish and hybrid grouper (Li et al, 2020;Wu et al, 2022). In the present study, functional experiments showed that the transcription of elovl8 was not significantly affected by feeding with A. salina, whereas it was inhibited after feeding with egg yolk in yellow catfish.…”

Section: Discussionsupporting

confidence: 40%

“…Further functional experiments indicated that Elovl8b but not Elovl8a possesses the capacity to biosynthesize HUFAs (Li et al, 2020). Consistently, a single elovl8b gene was identified in hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂), and functional experiments revealed that diets full of HUFAs can inhibit elovl8b transcription in liver, suggesting that a negative feedback regulation of the HUFA synthetic pathway existed (Wu et al, 2022). However, the evolutionary and functional characteristics of this new member are still largely unclear, and much more studies are required to illustrate these issues.…”

Section: Introductionmentioning

confidence: 73%

“…Tissue distribution experiments showed that elovl8b was widely expressed in various tissues with high expression levels in liver and intestine, which was different with rabbitfish and hybrid grouper (Li et al, 2020;Wu et al, 2022), indicating that the tissue distribution pattern of elovl8b is species-specific and it may play diverse roles in various teleosts. Indeed, this phenomenon may be caused by the different ability to synthesize HUFAs in different fishes (Gong et al, 2014).…”

Section: Discussionmentioning

confidence: 91%

“…Multiple protein sequence comparison showed that yellow catfish Elovl8 possessed all the characteristics of ELOVL protein family members, including four conserved regions (KXXEXXDT, QXXFLHXYHH, NXXXHXXMYXYY, and TXXQXXQ), endoplasmic reticulum (ER)-resident signals, six transmembrane regions, and a histidine cluster (HXXHH) that is involved in fatty acid elongation processes (Jakobsson et al, 2006). The secondary characteristics are similar to that of Elovl8b in the rabbitfish (Li et al, 2020) and hybrid grouper (Wu et al, 2022), implying the evolutionary and functional conservation of the Elovl8b among various teleosts.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco)

Zeng,

Wei,

Qin

et al. 2023

Front. Mar. Sci.

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Elongase of very long-chain fatty acid 8 (Elovl8) is a new member identified in the Elovl family that is involved in the synthesis of highly unsaturated fatty acids (HUFAs). However, the evolutionary and physiological roles of this enzyme are still largely unknown. In the present study, the elovl8 gene was identified and characterized from yellow catfish Pelteobagrus fulvidraco, and then its evolutionary and molecular characteristics as well as transcriptional changes in response to various nutritional status were determined. Results showed that the open reading frame (ORF) of elovl8 was 795 bp in length, encoding a protein of 264 amino acids. Multiple sequences alignment showed that the yellow catfish Elovl8 was highly conserved with other homologs in teleosts, sharing similar structural characteristics (including six conserved transmembrane α-helical domains, four conserved elongase motifs, and three highly conserved cysteine residues). Meanwhile, comparisons of genetic synteny confirmed that the elovl8 gene identified from the yellow catfish was the homolog of elovl8b in other teleosts, and thus, the elovl8a gene was lost in the genome of the yellow catfish. Gene structure analysis revealed that the elovl8b gene contained eight exons and seven introns, which was highly conserved in teleosts, implying the functional conservation among various fish species. Tissue distribution analysis detected by real-time quantitative PCR (RT-qPCR) showed that the elovl8 gene was extensively expressed in all detected tissues except eyes, with high expression levels in the intestine and liver. Temporal expression analysis revealed that the expression level of elovl8 was stably expressed in the early 12 h after fertilization, and then dramatically decreased at 24, 48, 72, and 96 h after fertilization, implying that elovl8 is required for HUFA biosynthesis in the early development stages. Functional experiments showed that the expression of the elovl8 gene was stimulated after feeding with egg yolk but was not obviously affected after feeding with halogenated worms, indicating that diets full of HUFAs can inhibit the expression of elovl8 in yellow catfish. Our findings will help us to better understand the evolutionary and functional characteristics of elovl8 in teleosts, and lay a solid basis for investigating the regulation mechanism of HUFA biosynthesis.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 40%