Molecular characterization, recombinant expression and bioactivity profile of an antimicrobial peptide, Ss-arasin from the Indian mud crab, Scylla serrata

Anju, A; Smitha, C.K.; Preetha, K.; Boobal, R.; Philip, Rosamma

doi:10.1016/j.fsi.2019.03.007

Cited by 20 publications

(9 citation statements)

References 17 publications

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“…A recombinant rSs-arasin was shown to inhibit human cervical carcinoma HeLa and colon carcinoma HT-29 cell growth. Further molecular studies are required to characterize the mechanisms by which rSs-arasin exerts cytotoxicity against cancer cells [ 110 ].…”

Section: Critical Issues Possible Solutions For Clinical Use Of Amentioning

confidence: 99%

Antimicrobial Peptides as Anticancer Agents: Functional Properties and Biological Activities

et al. 2020

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Antimicrobial peptides (AMPs), or host defense peptides, are small cationic or amphipathic molecules produced by prokaryotic and eukaryotic organisms that play a key role in the innate immune defense against viruses, bacteria and fungi. AMPs have either antimicrobial or anticancer activities. Indeed, cationic AMPs are able to disrupt microbial cell membranes by interacting with negatively charged phospholipids. Moreover, several peptides are capable to trigger cytotoxicity of human cancer cells by binding to negatively charged phosphatidylserine moieties which are selectively exposed on the outer surface of cancer cell plasma membranes. In addition, some AMPs, such as LTX-315, have shown to induce release of tumor antigens and potent damage associated molecular patterns by causing alterations in the intracellular organelles of cancer cells. Given the recognized medical need of novel anticancer drugs, AMPs could represent a potential source of effective therapeutic agents, either alone or in combination with other small molecules, in oncology. In this review we summarize and describe the properties and the mode of action of AMPs as well as the strategies to increase their selectivity toward specific cancer cells.

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Section: Critical Issues Possible Solutions For Clinical Use Of Amentioning

confidence: 99%

Antimicrobial Peptides as Anticancer Agents: Functional Properties and Biological Activities

et al. 2020

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“…This Scylla species of mud crab is in great demand as a quality food ingredient because of its size, nutritional content, and softness (Azra and Ikhwanuddin 2016). In addition, the sarasin contained in mud crabs is used as a therapeutic or prophylactic agent for disease control and health management (Anju et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Mangrove ecosystem in North Sumatran (Indonesia) forests serves as a suitable habitat for mud crabs (Scylla serrata and S. olivacea)

et al. 2021

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Abstract. Karniati R, Sulistiyono N, Amelia R, Slamet B, Bimantara Y, Basyuni M. 2021. Mangrove ecosystem in North Sumatran (Indonesia) forests serves as a suitable habitat for mud crabs (Scylla serrata and S. olivacea). Biodiversitas 22: 1489-1496. Mud crabs (Scylla serrata and Scylla olivacea) are often found in muddy substrates associated with mangrove vegetation. The habitat of these crabs ranges from tropical estuaries to sheltered subtropical areas, riverbanks, lower river traits, and intertidal areas. These crabs have an important economic value, and it increases the income of the communities living around the mangrove area. This study aims to obtain more information on the environmental factors of habitat for mud crabs (S. serrata and S. olivacea): insights on the effects of abiotic and biotic factors and potential economic value, in the village of Lubuk Kertang, Langkat, North Sumatra, Indonesia. Data were collected by measuring abiotic factors (temperature, pH, salinity, dissolved oxygen, mud depth, distance from the river) and biotic factors (mangrove stand density through canopy cover). The dataset was analyzed using a binary logistic regression analysis with a stepwise method. Results showed that the abiotic factors that contributed to the presence of the mud crab were dissolved oxygen and a mud depth of 68.7%. Meanwhile, the remaining 31.3% that contributed to the presence of mud crabs were abiotic factors (temperature, pH, salinity, distance from rivers) and biotic factors (stand density through canopy cover). The present study suggests that dissolved oxygen and mud depth have an important function in predicting the presence of mud crabs.

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“…When stimulating the mud crab with LPS, Anju et al (2019) increased the mRNA expression of the Scylla serrata hemocytes and increased the production of the peptide Ss-arasin. Ss-arasin is formed by 65 AAs with a predicted molecular weight of 7 kDa, and it was expressed in a prokaryotic heterologous system.…”

Section: Arthropoda Peptidesmentioning

confidence: 99%

Marine Organisms as a Rich Source of Biologically Active Peptides

et al. 2021

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Oceanic environments are one of the largest sources of bioactive molecules, due to the high degree of biodiversity and the innumerable ecological relationships established between macro and microorganisms found in the different ecosystems of these complex environments. Marine organisms are being studied increasingly because they are considered important producers of biologically active peptides. Peptides extracted from marine sources have different functions and structures, when compared to peptides isolated from terrestrial sources, considering the different adaptive pressures undergone by these organisms throughout the evolutionary process. Most bioactive compounds isolated from marine environments are obtained from symbiont microorganisms. Of these microorganisms, bacteria are an important source of bioactive peptides, isolated by metagenomic studies from complex gene networks expressed under marine conditions. Several peptides have been shown to have biotechnological properties such as antimicrobial, antitumor, antihypertensive, anticoagulant, anti-fouling, and antioxidant activity and can be used in the pharmaceutical and food industries. This review article aims to provide an overview of peptides of biotechnological importance, isolated from different phyla of marine organisms, examining the relationship between structure and function of some of these peptides, as well as the ways of extracting, purifying and prospecting new peptides by traditional methods of isolation or sequence analysis in databases. It also intends to list the peptides that are already being produced and used by the industry, in its various branches, and their current state in the market and in clinical tests.

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Molecular characterization, recombinant expression and bioactivity profile of an antimicrobial peptide, Ss-arasin from the Indian mud crab, Scylla serrata

Cited by 20 publications

References 17 publications

Antimicrobial Peptides as Anticancer Agents: Functional Properties and Biological Activities

Antimicrobial Peptides as Anticancer Agents: Functional Properties and Biological Activities

Mangrove ecosystem in North Sumatran (Indonesia) forests serves as a suitable habitat for mud crabs (Scylla serrata and S. olivacea)

Marine Organisms as a Rich Source of Biologically Active Peptides

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