Molecular characterization races of<i>Fusarium oxysporum</i>f.sp.<i>ciceri</i>using RAPD and ISSR markers

Katkar, Madhuri; Mane, S. S.; Kadam, Nivedita A

doi:10.5958/0976-0571.2015.00083.1

Cited by 5 publications

(3 citation statements)

References 8 publications

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“…Durai et al (2012) developed first time two sets of primer pairs SC-FOC1 and SC-FOC2 for identification of Foc population and they were checked against 36 collected Foc isolates representing different races present in India and confirmed the specificity of those markers. Katkar et al (2015) validated the specific SCAR primers for FOC (developed by Jimenez-Gasco and Jimenez-Diaz, 2003) and for all four Indian races such as race-1, 2, 3 and 4.…”

Section: Development and Validation Of Scar Markermentioning

confidence: 97%

Identification of Races of Fusarium oxysporum f.sp. ciceris, Inciting Wilt of Chickpea in Andhra Pradesh and Parts of Telangana

Venkataramanamma¹,

Reddy²,

Jayalakshmi³

et al. 2020

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Background: Fusarium oxysporum f.sp. ciceris (Foc) is one of the most important pathogen, causing wilt of chickpea. It is soil and seed borne pathogen. A serious threat in Foc is the evolution of new races, which reduces exploitation of wilt resistance in the crop in a particular area. Eight races (race 0, 1A, 1B/C, 2, 3, 4, 5 and 6) were reported in the world and among them four races (race 1, 2, 3, 4) were from India. Race 1 was reported from Andhra Pradesh. It is very important to monitor the variation regularly in new isolates collected from different varieties or genotypes and different geographical regions to identify the racial pattern. Development of SCAR markers for identification of Foc isolates is also important as they are highly reliable. Methods: Twenty isolates of Fusarium oxysporum f.sp. ciceris were obtained from wilt infected plants of chickpea covering different places of Andhra Pradesh including two isolates from Telangana and confirmed the pathogen based on pathogenicity test. They were tested on host differentials of chickpea for races identification. Among these twenty isolates five most virulent isolates (Foc-6, Foc-10, Foc-12, Foc-17 and Foc-24) were selected for RAPD based on pathogenicity test and SCAR marker was developed based on DNA banding pattern during RAPD for one isolate.Result: Based on the disease reaction on differentials, concluded that 17 of them out of 20 were matched with race-1 reaction. Two isolates were matched with race-6 reaction and one is not matched with none of the races. An amplified product of polymorphic band of approximately 700 bp in the isolate Foc-12, obtained during RAPD analysis was selected for SCAR marker development and two SCAR markers were developed and validated. Identification of races mainly helps in development of resistant cultivars to specific races and might be contributed to development of integrated disease management practices for Fusarium wilt.

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Section: Development and Validation Of Scar Markermentioning

confidence: 97%

Identification of Races of Fusarium oxysporum f.sp. ciceris, Inciting Wilt of Chickpea in Andhra Pradesh and Parts of Telangana

Venkataramanamma¹,

Reddy²,

Jayalakshmi³

et al. 2020

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“…More and more technical methods have been applied to solve better confused circumscription of species. Morphological traits (Abozeid et al 2018;Binzat et al 2014), biochemical methods (Leht and Jaaska, 2002;Pal et al 2010) and molecular characteristics (Choi et al 2006;Schaefer et al 2012;Shiran et al 2014;Katkar et al 2015;Narula et al 2013;Prasanthi et al 2009) have been used to determine the inter specific relationships within the genus Vicia. Kupicha (1976) divided the world-wide species of genus Vicia into subgenus Vicia (5 sections) and subgenus Vicilla (17 sections) (Jaaska, 2005) by morphological traits.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Relationships in Vicia Subgenus Vicilla (Fabaceae) Based on Combined Evidence from DNA Sequences

Wu¹,

Sun²,

Liu³

et al. 2021

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Background: Vicia L. is rich in protein and can be used as a good forage, green manure, etc., but its classification is chaotic, which is not conducive to breeding and utilization. The purpose of the study is sequencing the nrDNA ITS region the chloroplast region (matK, rbcL and trnL-F) of the Vicia species of subgenus Vicilla of Vicia to resolve taxonomic contradictions.Methods: Collecting seeds from the National Herbage Germplasm Conservation Centre of China (Beijing, China) and the germplasm bank of wild species (Kunming, China). The seedlings were cultivated in greenhouse as experimental materials. The nrDNA ITS region and the chloroplast region (matK, rbcL and trnL-F) were sequenced in 17 Vicia species of subgenus Vicilla of Vicia. Maximum parsimony (MP), maximum likelihood (ML) and Bayesian inference (BI) for all the data sets were used to estimate the individual and multilocus phylogenetic trees are used in this study and the result showed a highly-resolved phylogeny of the Vicia species. Result: V. amurensis, V. costata and V. tibetica belong to section Vicilla. Sections Cassubicae, Americanae, Variegatae and Lenticula were monophyletic groups. The sections Vicilla, Cracca and Amurense appeared polyphyletic. Treatment of sections Lenticula and Ervumin a separate subgenus Ervum was not supported. The research evaluated the phylogenetic position of the subgenus Vicilla species and provides a theoretical basis for the precise classification of the subgenus Vicilla and the genus Vicia.

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“…Pathogenic fungi colonizing pea seeds are usually identified during macroscopic and microscopic observations based on their morphology (Kulik, 2005). However, conventional methods are increasingly replaced with molecular techniques which are faster and deliver more reliable results (Katkar et al 2015;Schilling et al 1996). Diagnostic procedures involving observations of pathogens that colonize plants and seeds during the growing season significantly contribute to disease prevention in peas grown for food, feed and sowing.…”

Section: Introductionmentioning

confidence: 99%

The frequency of occurrence of pathogenic and saprotrophic fungi in pea seeds in different regions of Poland

kowska

Okorski

Fordoñski

et al. 2018

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Field pea seeds (cv. Tarchalska) grown in different regions of Poland (Warmia and Mazury, Wielkopolska, Kujawy) were subjected to mycological analyses. Simpson’s dominance index (DI) was used in the study to determine the abundance of each fungal species in the fungal communities isolated from seeds. PCR analyses were carried out with the use of species-specific primers for Fusarium fungi isolated from pea seeds to identify Tri genes responsible for trichothecene synthesis in the growing seasons of 2011, 2012 and 2013.Saprotrophic species predominated on pea seeds cv. Tarchalska grown in various Polish regions, whereas the isolated fungal pathogens were represented by species of the genus Fusarium and Botrytis cinerea. Genes encoding the synthesis of trichothecenes, the mycotoxins produced by Fusarium fungi, were not identified in the PCR analysis. In the group of peas grown in three Polish regions (Warmia and Mazury, Wielkopolska, Kujawy), the highest level of fungal colonization was noted in the pea seeds harvested in Warmia and Mazury.

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Molecular characterization races ofFusarium oxysporumf.sp.ciceriusing RAPD and ISSR markers

Cited by 5 publications

References 8 publications

Identification of Races of Fusarium oxysporum f.sp. ciceris, Inciting Wilt of Chickpea in Andhra Pradesh and Parts of Telangana

Identification of Races of Fusarium oxysporum f.sp. ciceris, Inciting Wilt of Chickpea in Andhra Pradesh and Parts of Telangana

Phylogenetic Relationships in Vicia Subgenus Vicilla (Fabaceae) Based on Combined Evidence from DNA Sequences

The frequency of occurrence of pathogenic and saprotrophic fungi in pea seeds in different regions of Poland

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