Molecular Characterization, Phylogenetic Relationships, and Specific Detection of <i>Peach mosaic virus</i>

James, D.; Varga, Á.; Croft, H.; Rast, Heidi; Thompson, Dan; Hayes, Shirley J.

doi:10.1094/phyto-96-0137

Cited by 12 publications

(3 citation statements)

References 23 publications

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“…Similar proteins are found in some, but not all, viruses in the genus Trichovirus (54). It was recently shown that the nucleic acid-binding protein of GVA is able to suppress RNA silencing (127), suggesting that this function may be conserved in other related proteins of flexiviruses.…”

Section: -Proximal Orfs Encoding Capsid and Other Proteinsmentioning

confidence: 69%

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

Martelli¹,

Adams

Kreuze

et al. 2007

Annu. Rev. Phytopathol.

319

196

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The plant virus family Flexiviridae includes the definitive genera Potexvirus, Mandarivirus, Allexivirus, Carlavirus, Foveavirus, Capillovirus, Vitivirus, Trichovirus, the putative genus Citrivirus, and some unassigned species. Its establishment was based on similarities in virion morphology, common features in genome type and organization, and strong phylogenetic relationships between replicational and structural proteins. In this review, we provide a brief account of the main biological and molecular properties of the members of the family, with special emphasis on the relationships within and among the genera. In phylogenetic analyses the potexvirus-like replicases were more closely related to tymoviruses than to carlaviruses. We postulate a common evolutionary ancestor for the family Tymoviridae and the two distinct evolutionary clusters of the Flexiviridae, i.e., a plant virus with a polyadenylated genome, filamentous virions, and a triple gene block of movement proteins. Subsequent recombination and gene loss would then have generated a very diverse group of plant and fungal viruses.

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Section: -Proximal Orfs Encoding Capsid and Other Proteinsmentioning

confidence: 69%

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

Martelli¹,

Adams

Kreuze

et al. 2007

Annu. Rev. Phytopathol.

319

196

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“…CMLV is taxonomically and serologically related to PcMoV [7]. In contrast, the intergenic region between ORFs 3 and 4 is more closely related to that of CMLV-SA1162-21 (77 % identity) than to that of PcMoV (48 % identity).…”

mentioning

confidence: 99%

Complete nucleotide sequence of a strain of cherry mottle leaf virus associated with peach wart disease in peach

et al. 2013

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The complete nucleotide sequence and genome organization of a peach virus isolate from a naturally infected peach tree showing typical peach wart-like symptoms on the fruit surface was determined and compared to sequences of members of the family Betaflexiviridae. The genome consists of 7,987 nucleotides, excluding the poly-A tail, and has four open reading frames (ORFs). Analysis of the whole genome and putative proteins encoded by each ORF revealed greatest sequence similarity to a cherry isolate of cherry mottle leaf virus (CMLV). The two isolates have similar genome organizations and share 88 and 93 % homology in their corresponding products of the replicase and coat protein genes, respectively. CMLV has been reported from several Prunus spp. and may be associated with peach wart-like disease symptoms on peach fruit.Electronic supplementary materialThe online version of this article (doi:10.1007/s00705-013-1698-3) contains supplementary material, which is available to authorized users.

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“…The PcMV-specific fragments were amplified making this protocol to be very useful for specific detection of this virus (Fig. 2.7) (James et al 2006).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Molecular Techniques for Detection of Microbial Pathogens

Narayanasamy

Molecular Biology in Plant Pathogenesis and Disease Management

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Detection, identification and differentiation of microbial plant pathogens -oomycetes, fungi, bacteria, phytoplasmas, viruses and viroids -infecting various crops constitute the basic step for the development of effective crop disease management systems. The conventional cultural methods involving isolation and studying the morphological characteristics using microscope are labor intensive and cumbersome, yielding sometimes, inconclusive results. The molecular techniques, on the other hand, are able to provide precise, reliable and reproducible results rapidly, facilitating early disease management decisions. Biochemical, immunological and nucleic acid-based assays have been preferred, because of the distinct advantages over the conventional methods. The molecular techniques have been very useful in the identification of obligate pathogens causing diseases such as downy mildews, powdery mildews and rusts and also fungi that grow very slowly in the culture media, taking several weeks to produce spore forms that can be used for identification. The availability of antisera, primers and commercial kits has led to widespread application of the molecular techniques for on-site detection during field surveys for assessing the distribution of existing pathogens, occurrence of new/introduced pathogens or strains and for preventing introduction of new pathogens through seeds or planting materials. Furthermore, molecular techniques have been demonstrated to be useful in breeding programs to identify sources of resistance to disease(s). Several protocols for molecular techniques, useful for researchers and students are presented in the Appendix.

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Molecular Characterization, Phylogenetic Relationships, and Specific Detection of Peach mosaic virus

Cited by 12 publications

References 23 publications

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

Complete nucleotide sequence of a strain of cherry mottle leaf virus associated with peach wart disease in peach

Molecular Techniques for Detection of Microbial Pathogens

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