Molecular characterization of β-tubulin gene associated with benzimidazole resistance in larvae of field isolates of Parascaris (Nematoda: Ascarididae)

Malekpour, Seyed Hossein; Rakhshandehroo, Ehsan; Yektaseresht, Azadeh

doi:10.1007/s12639-019-01146-y

Cited by 7 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tradition in veterinary parasitology has been to refer to one equine ascarid species, Parascaris equorum . However, the published literature contains scant mention of a cryptic ascarid species infecting horses, named Parascaris univalens [ 141 , 142 ], which is the dominating species infecting horses in Sweden, Switzerland and the USA, and possibly globally [ 143 ]. A sequence of the mitochondrial cytochrome c oxidase subunit 2 ( cox 2) gene (GenBank: MG676884) from Iranian isolates shows > 99% identity with sequences for both P. equorum and P. univalens available on GenBank [ 144 ].…”

Section: Helminthosesmentioning

confidence: 99%

Parasitic diseases of equids in Iran (1931–2020): a literature review

et al. 2020

View full text Add to dashboard Cite

Parasitic infections can cause many respiratory, digestive and other diseases and contribute to some performance conditions in equids. However, knowledge on the biodiversity of parasites of equids in Iran is still limited. The present review covers all the information about parasitic diseases of horses, donkeys, mules and wild asses in Iran published as articles in Iranian and international journals, dissertations and congress papers from 1931 to July 2020. Parasites so far described in Iranian equids include species of 9 genera of the Protozoa (Trypanosoma, Giardia, Eimeria, Klossiella, Cryptosporidium, Toxoplasma, Neospora, Theileria and Babesia), 50 helminth species from the digestive system (i.e., 2 trematodes, 3 cestodes and 37 nematodes) and from other organs (i.e., Schistosoma turkestanica, Echinococcus granulosus, Dictyocaulus arnfieldi, Parafilaria multipapillosa, Setaria equina and 3 Onchocerca spp.). Furthermore, 16 species of hard ticks, 3 mite species causing mange, 2 lice species, and larvae of 4 Gastrophilus species and Hippobosca equina have been reported from equids in Iran. Archeoparasitological findings in coprolites of equids include Fasciola hepatica, Oxyuris equi, Anoplocephala spp. and intestinal strongyles. Parasitic diseases are important issues in terms of animal welfare, economics and public health; however, parasites and parasitic diseases of equines have not received adequate attention compared with ruminants and camels in Iran. The present review highlights the knowledge gaps related to equines about the presence, species, genotypes and subtypes of Neospora hughesi, Sarcocystis spp., Trichinella spp., Cryptosporidium spp., Giardia duodenalis, Blastocystis and microsporidia. Identification of ticks vectoring pathogenic parasites, bacteria and viruses has received little attention, too. The efficacy of common horse wormers also needs to be evaluated systematically.

show abstract

Section: Helminthosesmentioning

confidence: 99%

Parasitic diseases of equids in Iran (1931–2020): a literature review

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The AR of GINs against benzimidazole compounds in sheep [ 7 ] and horses [ 13 ] in Iran has previously been reported, but to our knowledge no previous data have been published on the field efficacy of benzimidazole compounds in poultry.…”

Section: Discussionmentioning

confidence: 99%

“…In Iran, studies assessing the field efficacy of anthelmintics and the detection of anthelmintic resistance have been performed since 2007 [7]. Anthelmintic resistance (AR) in Iran has been described in gastrointestinal nematodes of sheep (benzimidazole and levamisole resistance) [7][8][9][10][11][12] and horses (benzimidazole resistance) [13]. Notably, some evidence on AR was not accessible because of the inevitable conditions (e.g.…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic efficacy of fenbendazole and levamisole in native fowl in northern Iran

2021

View full text Add to dashboard Cite

Background With the increasing number of free-range domestic chickens, helminth parasites have potentially become more of a threat to commercial flocks in recent years, and routine poultry deworming is needed to improve the efficiency of chicken production. The present study deals with a field trial to study the efficacy of two generally used anthelmintics, fenbendazole and levamisole, against gastrointestinal nematodes of domestic chickens in northern Iran. Methods Of 45 domestic chicken flocks involved in the study, 20 flocks were selected to participate in fecal egg count reduction testing based on flock size from April 2017 to September 2018. The infected chickens were randomly divided into three equal groups of 30 each. Ninety chickens in the infected groups received one of the following treatments (d 0 of treatment): Group 1: 5 mg kg−1 body weight (BW) fenbendazole for 3 consecutive days; Group 2: 16 mg kg−1 BW levamisole; Group 3 control: placebo, water + DMSO (dimethylsulfoxide). The efficacy of the treatments was evaluated by comparing fecal egg counts in the treated and control groups. Results Examination of three flocks of chickens from the control group showed that 95.0% of the animals were infected with gastrointestinal nematodes with an average geometric value of 361 eggs per gram of feces. Fenbendazole at a dose of 5 mg kg−1 BW for 3 days showed an efficacy of 83.7% (P ≥ 0.05), and levamisole at a dose of 16 mg kg−1 BW showed 71.8% efficacy (P ≥ 0.05) with geometric mean eggs in a gram of feces of 100 and 199.6, respectively. In general, fenbendazole and levamisole treatment led to significantly lower activity. The result of this study revealed that fenbendazole was a better and more effective dewormer than levamisole on the three Iranian domestic chicken flocks, but the difference was not significant. Capillaria spp. were the most generally resistant nematodes followed by Trichostrongylus spp. and Amidostomum anseris. Conclusion Our results indicated that fenbendazole and levamisole effectively reduced the number of nemathodes in three Iranian domestic chicken flocks. Given the results of our study, resistance can be expected in the parasitic helminths of poultry. Additional larger scale studies are required to determine the prevalence of anthelmintic resistance in the poultry industry.

show abstract

“…Previously, AR in GINs of sheep [8] and horses [14] had been reported from Iran against benzimidazoles compound, but no previous data has been published on the eld e cacy of benzimidazole compound in poultry.…”

Section: Discussionmentioning

confidence: 99%

“…In Iran, studies assessing the eld e ciency of anthelmintics and the detection of anthelminthic resistance have been performed since 2007 [8]. Anthelmintic resistance in Iran has been described in gastrointestinal nematodes of sheep (benzimidazole and Levamisole resistance) [8][9][10][11][12][13] and horse (benzimidazole) [14]. It should be noted that some evidence on AR was not accessible due to inevitable conditions (e.g., information not released for consideration by scientists, etc.).…”

Section: Introductionmentioning

confidence: 99%

Anthelminthic Efficacy of Fenbendazole and Levamisole in Native Fowl in Northern Iran

Soudkolaei

Kalidari

Borji

2020

Preprint

View full text Add to dashboard Cite

Background: With the number of free-range domestic chickens increasing, helminth parasites have potentially become more of a threat in commercial flocks in recent years, and routine poultry worming is needed to improve chicken products. The present study deals with a field trial to study the efficiency of two generally used anthelmintics as fenbendazole and levamisole, against gastrointestinal nematodes of domestic chickens in the north of Iran. Methods: Out of 45 domestic chickens flocks that agreed to the study, 20 flocks were selected to participate in fecal egg count reduction testing based on flock size from April 2017 to September 2018. The infected chickens were randomly distributed into three equal groups of each (n = 30). Ninety chickens in the infected groups received one of the following treatments (d 0 of treatment): Group 1: 5 mg kg−1 body weight (BW) of fenbendazole for three continuous days, Group 2: 16 mg kg−1 BW of Levamisole, and Group 3: placebo; water + DMSO (the control). The efficiency of the treatments was evaluated by comparing fecal egg count in the treated groups and control group.Results: Examination of 3 herds of chickens from the control group showed that the animals were infected with gastrointestinal nematodes by 95.0% with an average geometric value of 361 eggs per gram of feces. The substance fenbendazole at a dose of 5 mg/kg b/w for three days against gastrointestinal nematodes showed an efficiency of 83.7% (P ≥0.05), and the substance levamisole at a dose of 16 mg/kg b/w showed 71.8% (P ≥0.05) with geometric mean eggs in a gram of feces 100 and 199.6, respectively. In general, the initial substances of fenbendazole and leamisole showed significantly lower activity.. The result of this study revealed that fenbendazole was a better effective dewormer than levamisole on the three Iranian domestic chicken flocks, but it is not significant. Capillaria spp., were the most generally resistant nematodes followed by Trichostrongylus spp., and Amidostomum anseris.Conclusion: Our results indicated there is a reduction efficacy in fenbendazole and levamisole in three Iranian domestic chicken flocks. Given the results of our study, it appears possible that resistance may be expected in the helminth parasite of poultry. Additional studies with a larger scale are required to determine the prevalence of anthelminthic resistance in the poultry industry.

show abstract

Molecular characterization of β-tubulin gene associated with benzimidazole resistance in larvae of field isolates of Parascaris (Nematoda: Ascarididae)

Cited by 7 publications

References 23 publications

Parasitic diseases of equids in Iran (1931–2020): a literature review

Parasitic diseases of equids in Iran (1931–2020): a literature review

Anthelmintic efficacy of fenbendazole and levamisole in native fowl in northern Iran

Anthelminthic Efficacy of Fenbendazole and Levamisole in Native Fowl in Northern Iran

Contact Info

Product

Resources

About