Molecular characterization of woodchuck interleukin 15 (wIL-15) and detection of its expression in liver samples of woodchucks infected with woodchuck hepatitis virus (WHV)

Wang, Baoju; Lohrengel, Beate; Lu, Yinping; Zhao, Meng; Xu, Yu; Yang, Dongliang; Roggendorf, M; Lu, Mengji

doi:10.1016/j.cyto.2005.11.007

Cited by 10 publications

(4 citation statements)

References 52 publications

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“…Important woodchuck cytokines and their receptors that have been cloned so far are summarized in Table 1. Among them, tumor necrosis factor (TNF)-a, IFN-a, IFN-g, interleukin (IL)-8, IL-12, IL-15, granulocyte-macrophage colony-stimulating factor (GM-CSF), lymphotoxin (LT)-a, and IL-10R have been tested for their biological activities (Lohrengel et al 1998(Lohrengel et al , 2000Li et al 2000;Garcia-Navarro et al 2001;Wu et al 2001;Lu et al 2002;Wang et al 2005;Liu et al 2009;Jiang et al 2012). The entire open reading frame (ORF) of IFN-a was cloned under control of the cytomegalovirus (CMV) promoter into the expression vector PVIJ, and the biological action of IFN-a was shown by inducing expression of MxA-protein.…”

Section: Cloning and Characterization Of Components Of The Woodchuck mentioning

confidence: 99%

The Woodchuck, a Nonprimate Model for Immunopathogenesis and Therapeutic Immunomodulation in Chronic Hepatitis B Virus Infection

Roggendorf¹,

Kosinska²,

Liu³

et al. 2015

Cold Spring Harb Perspect Med

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The woodchuck hepatitis virus (WHV) and its host, the eastern woodchuck, is a very valuable model system for hepatitis B virus infection. Many aspects of WHV replication and pathogenesis resemble acute and chronic hepatitis B infection in patients. Since the establishment of immunological tools, woodchucks were used to develop new therapeutic vaccines and immunomodulatory approaches to treat chronic hepadnaviral infections. Combination therapy of nucleos(t)ide analogs, with prime-boost vaccination and triple therapy, including immunomodulatory strategies by blocking the interaction of the programmed death-1 (PD-1) receptor with its ligand inducing a potent T-cell response in chronic WHV carrier woodchucks, suppression of viral replication, and complete elimination of the virus in 30% of the animals. Both strategies may be used for future therapies in patients with chronic hepatitis B.

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Section: Cloning and Characterization Of Components Of The Woodchuck mentioning

confidence: 99%

The Woodchuck, a Nonprimate Model for Immunopathogenesis and Therapeutic Immunomodulation in Chronic Hepatitis B Virus Infection

Roggendorf¹,

Kosinska²,

Liu³

et al. 2015

Cold Spring Harb Perspect Med

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“…Besides the genes described in the present study, the alpha interferon (IFN-a) system of M. himalayana was characterized by molecular cloning and sequence analysis (Lu et al, 2008). monax (Lohrengel et al, 1998;Wang et al, 2005). These data suggest again the common origin and a short In the present study, three different Chinese Marmotaspecies were tested for their susceptibility.…”

Section: Passage Of Whv In M Himalayanamentioning

confidence: 95%

Establishing a new animal model for hepadnaviral infection: susceptibility of Chinese Marmota-species to woodchuck hepatitis virus infection

Wang

Tian

Zhao

et al. 2010

Journal of General Virology

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Hepatitis B virus infection (HBV) is a major medical problem in China. The lack of a suitable infection model in China is recognized as an obstacle for research on HBV in China. Chinese Marmota-species is phylogenetically closely related to Marmota monax, thus, it might be suitable to serve as an animal model for HBV infection. Therefore, we attempted to prove the claim about the existence of woodchuck hepatitis virus (WHV)-like viruses in Chinese Marmota-species and to determine the susceptibility of these species to experimental WHV infection. In the present study, 653 sera from three Chinese Marmota-species, Marmota himalayana, Marmota baibacina and Marmota bobak, were screened for WHV-like viruses by serological and molecular assays. The susceptibility to WHV of three species was investigated by experimental infection and monitored by testing of anti-WHc and WHsAg by ELISA, detection of WHV DNA by PCR, and detection of WHV replication intermediates and antigens in liver samples. No evidence for the existence of a genetically closely related virus to WHV in three Chinese Marmota-species was found by serological assays and PCR. M. himalayana was susceptible to WHV infection as inoculated animals became positive for anti-WHc, WHsAg and WHV DNA. Further, WHV replication intermediates and proteins were detected in liver samples. In contrast, M. baibacina remained negative for tested virological parameters. M. bobak species showed a limited susceptibility to WHV. Our data do not support early reports about WHV-like viruses in China. M. himalayana is suitable for the establishment of a model for hepadnaviral infection.

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“…himalayana are closely related to M . monax , as the genes of the immune system of both species are nearly identical in their nucleotide sequences, as analyzed so far [13–15]. However, the transcriptional comparison analysis between the two species was restricted to only a few sequenced gene segments due to the lack of accessible genomic resources for both species.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis and Comparison of Marmota monax and Marmota himalayana

Liu

Wang

et al. 2016

PLoS ONE

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The Eastern woodchuck (Marmota monax) is a classical animal model for studying hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC) in humans. Recently, we found that Marmota himalayana, an Asian animal species closely related to Marmota monax, is susceptible to woodchuck hepatitis virus (WHV) infection and can be used as a new mammalian model for HBV infection. However, the lack of genomic sequence information of both Marmota models strongly limited their application breadth and depth. To address this major obstacle of the Marmota models, we utilized Illumina RNA-Seq technology to sequence the cDNA libraries of liver and spleen samples of two Marmota monax and four Marmota himalayana. In total, over 13 billion nucleotide bases were sequenced and approximately 1.5 billion clean reads were obtained. Following assembly, 106,496 consensus sequences of Marmota monax and 78,483 consensus sequences of Marmota himalayana were detected. For functional annotation, in total 73,603 Unigenes of Marmota monax and 78,483 Unigenes of Marmota himalayana were identified using different databases (NR, NT, Swiss-Prot, KEGG, COG, GO). The Unigenes were aligned by blastx to protein databases to decide the coding DNA sequences (CDS) and in total 41,247 CDS of Marmota monax and 34,033 CDS of Marmota himalayana were predicted. The single nucleotide polymorphisms (SNPs) and the simple sequence repeats (SSRs) were also analyzed for all Unigenes obtained. Moreover, a large-scale transcriptome comparison was performed and revealed a high similarity in transcriptome sequences between the two marmota species. Our study provides an extensive amount of novel sequence information for Marmota monax and Marmota himalayana. This information may serve as a valuable genomics resource for further molecular, developmental and comparative evolutionary studies, as well as for the identification and characterization of functional genes that are involved in WHV infection and HCC development in the woodchuck model.

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Molecular characterization of woodchuck interleukin 15 (wIL-15) and detection of its expression in liver samples of woodchucks infected with woodchuck hepatitis virus (WHV)

Cited by 10 publications

References 52 publications

The Woodchuck, a Nonprimate Model for Immunopathogenesis and Therapeutic Immunomodulation in Chronic Hepatitis B Virus Infection

The Woodchuck, a Nonprimate Model for Immunopathogenesis and Therapeutic Immunomodulation in Chronic Hepatitis B Virus Infection

Establishing a new animal model for hepadnaviral infection: susceptibility of Chinese Marmota-species to woodchuck hepatitis virus infection

Transcriptome Analysis and Comparison of Marmota monax and Marmota himalayana

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