Molecular characterization of veterinary important trematode and cestode species in the mithun Bos frontalis from north-east India

Abstract

Helminth infections in the mithun Bos frontalis, including the liver fluke Fasciola gigantica, hepato-gastric amphistomes Explanatum explanatum, Paramphistomum epiclitum and Calicophoron calicophorum, and the cestodes Echinococcus granulosus and E. ortleppi were studied in north-east India over a 2-year period from 2012 to 2014. Cystic echinococcosis caused by E. granulosus and E. ortleppi was found to be highly prevalent in the mithun, with E. ortleppi being reported for the first time. Molecular markers, including the internal transcribed spacer 2 (ITS-2), 28S rDNA and mitochondrial NADH dehydrogenase sub-unit1 (nad1) were used to confirm the identification of the trematode and cestode species.

Molecular confirmation of trematodes in the snail intermediate hosts from Ratchaburi Province, Thailand

“…Conventional PCR methods have been widely applied for the purposes of identification because the DNA method is very sensitive, highly accurate and can be rapidly applied. Therefore, the sequence data have proven to be helpful in the study of species identification and geographical distribution for numerous species such as Paragonimus westermani, Fasciolopsis buski and Fasciola gigantica [20], Explanatum explanatum, Paramphistomum epiclitum and Calicophoron calicophorum [25].…”

Pathological Studies of Mecistocirrus digitatus Infection in Naturally Infested Mithun and Phere (Cross Bred of Mithun)

“…Among all helminth parasites, Haemonchus and Mecistocirrus group of Trichostrongylidae family can be considered as number one killers in calves. The occurrence of different helminth parasites in mithun was reviewed by different authors from time to time (Chamuah et al, 2009;and Chamuah et al, 2014, Chamuah et al, 2015aChamuah et al, 2015b). The present study was carried out to investigate the pathological alterations of different organ in parasite infested animals.…”

Parasites of mithun (Bos frontalis) in North Eastern hilly region of India-A review

“…Pimply gut condition caused by Oesophagostomum spp. is characterized by nodule formation in the large intestine and is also very common in mithun calves in India (Chamuah et al 2016c). Toxocarosis is responsible for calf mortality in mithun, with significant changes in haemoto-biological parameters being recorded in calves (Rajkhowa et al 2003).…”

“…The associated pathological conditions like cirrhosis and fibrosis of hepatic lobule were also documented (Chamuah, 2005). Molecular markers, including the internal transcribed spacer 2 (ITS-2), 28S rDNA and mitochondrial NADH dehydrogenase sub-unit1 (nad1) were also used to confirm the identification of the trematode and cestode species (Chamuah et al 2016c). Cestode infection is also a common ailment in both mithun calves and adults.…”

“…This tick species was reported on mithun in Nagaland by Chamuah et al (2016b). Based on the morphological features and sequencing of internal transcribed spacer-2, the tick species of mithun were identified as I. acutitarsus and I. ovatus (Chamuah et al, 2016c). Rhipicephalus microplus is the other common tick species infesting mithun with highest prevalence reported in summer season (Rajkhowa et al 2005b;Chamuah et al 2012;Chamuah et al 2013b).…”