Molecular characterization of vernalization loci VRN1 in wild and cultivated wheats

Golovnina, Kseniya; Kondratenko, E. Ya.; Блинов, А. Г.; Гончаров, Н. П.

doi:10.1186/1471-2229-10-168

Cited by 63 publications

(113 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ex Gandil. is identical to vrn-A1 of winter accessions of polyploid wheats and differs from vrn-A m 1 by a short deletion in the promoter [16, 17]. The vrn-A m 1b with a 48-bp deletion in the VRN1 promoter in compare to vrn-A m 1 so far was found only in accessions of T. monococcum L. [3, 18].…”

Section: Introductionmentioning

confidence: 99%

“…Two dominant alleles from diploid wild wheat T. boeoticum Boiss. Vrn-A m 1f and Vrn-A m 1a ( Vrn-A1h ) posses short deletions in the promoter region [16, 17]. No dominant alleles were identified in T. urartu [16, 17].…”

Section: Introductionmentioning

confidence: 99%

“…five recessive alleles of VRN1 genes ( vrn-A1 ( vrn-A1u ), vrn-A1b.3 , vrn-A1b.4 , vrn-B1 and vrn-G1 ) were identified [16, 17, 19]. The sequences of vrn-B1 and vrn-G1 alleles were identical to each other [16]. Several dominant and recessive alleles of VRN-A1 , VRN-B1 and VRN-G1 genes were identified in tetraploid wheat of two sections Timopheevii A. Filat.…”

Section: Introductionmentioning

confidence: 99%

“…and T. araraticum Jakubz. revealed one dominant allele of the VRN-A1 gene ( Vrn-A1f ) comprising two deletions in the promoter region, and the only dominant allele of VRN-G1 gene ( Vrn-G1a ) with the insertion in the promoter region [16]. Ten various dominant alleles of the VRN-A1 gene which possess different mutations in compare to vrn-A1 allele were identified in different tetraploid wheat species of section Dicoccoides Flaksb.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

VRN1 genes variability in tetraploid wheat species with a spring growth habit

et al. 2016

View full text Add to dashboard Cite

BackgroundVernalization genes VRN1 play a major role in the transition from vegetative to reproductive growth in wheat. In di-, tetra- and hexaploid wheats the presence of a dominant allele of at least one VRN1 gene homologue (Vrn-A1, Vrn-B1, Vrn-G1 or Vrn-D1) determines the spring growth habit. Allelic variation between the Vrn-1 and vrn-1 alleles relies on mutations in the promoter region or the first intron. The origin and variability of the dominant VRN1 alleles, determining the spring growth habit in tetraploid wheat species have been poorly studied.ResultsHere we analyzed the growth habit of 228 tetraploid wheat species accessions and 25 % of them were spring type. We analyzed the promoter and first intron regions of VRN1 genes in 57 spring accessions of tetraploid wheats. The spring growth habit of most studied spring accessions was determined by previously identified dominant alleles of VRN1 genes. Genetic experiments proof the dominant inheritance of Vrn-A1d allele which was widely distributed across the accessions of Triticum dicoccoides. Two novel alleles were discovered and designated as Vrn-A1b.7 and Vrn-B1dic. Vrn-A1b.7 had deletions of 20 bp located 137 bp upstream of the start codon and mutations within the VRN-box when compared to the recessive allele of vrn-A1. So far the Vrn-A1d allele was identified only in spring accessions of the T. dicoccoides and T. turgidum species. Vrn-B1dic was identified in T. dicoccoides IG46225 and had 11 % sequence dissimilarity in comparison to the promoter of vrn-B1. The presence of Vrn-A1b.7 and Vrn-B1dic alleles is a predicted cause of the spring growth habit of studied accessions of tetraploid species. Three spring accessions T. aethiopicum K-19059, T. turanicum K-31693 and T. turgidum cv. Blancal possess recessive alleles of both VRN-A1 and VRN-B1 genes. Further investigations are required to determine the source of spring growth habit of these accessions.ConclusionsNew allelic variants of the VRN-A1 and VRN-B1 genes were identified in spring accessions of tetraploid wheats. The origin and evolution of VRN-A1 alleles in di- and tetraploid wheat species was discussed.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0924-z) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

VRN1 genes variability in tetraploid wheat species with a spring growth habit

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Another explanation is that alternative genes that reduce the vernalization requirement might compromise other important traits (e.g., grain yield), and so are not used in wheat breeding. A final explanation is that active alleles of VRN1 (and VRN4) might have been common in wild wheats at the dawn of agriculture, and so were easily incorporated into cultivated varieties (13). The vernalization requirement is an important trait, so it will be worthwhile to explore other ways to breed wheats that flower without vernalization.…”

mentioning

confidence: 99%

Wheat gene for all seasons

Trevaskis¹

2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

CaAP2 transcription factor is a candidate gene for a flowering repressor and a candidate for controlling natural variation of flowering time in Capsicum annuum

et al. 2015

View full text Add to dashboard Cite

The APETALA2 transcription factor homolog CaAP2 is a candidate gene for a flowering repressor in pepper, as revealed by induced-mutation phenotype, and a candidate underlying a major QTL controlling natural variation in flowering time. To decipher the genetic control of transition to flowering in pepper (Capsicum spp.) and determine the extent of gene function conservation compared to model species, we isolated and characterized several ethyl methanesulfonate (EMS)-induced mutants that vary in their flowering time compared to the wild type. In the present study, we report on the isolation of an early-flowering mutant that flowers after four leaves on the primary stem compared to nine leaves in the wild-type 'Maor'. By genetic mapping and sequencing of putative candidate genes linked to the mutant phenotype, we identified a member of the APETALA2 (AP2) transcription factor family, CaAP2, which was disrupted in the early-flowering mutant. CaAP2 is a likely ortholog of AP2 that functions as a repressor of flowering in Arabidopsis. To test whether CaAP2 has an effect on controlling natural variation in the transition to flowering in pepper, we performed QTL mapping for flowering time in a cross between early and late-flowering C. annuum accessions. We identified a major QTL in a region of chromosome 2 in which CaAP2 was the most significant marker, explaining 52 % of the phenotypic variation of the trait. Sequence comparison of the CaAP2 open reading frames in the two parents used for QTL mapping did not reveal significant variation. In contrast, significant differences in expression level of CaAP2 were detected between near-isogenic lines that differ for the flowering time QTL, supporting the putative function of CaAP2 as a major repressor of flowering in pepper.

show abstract

Molecular characterization of vernalization loci VRN1 in wild and cultivated wheats

Cited by 63 publications

References 46 publications

VRN1 genes variability in tetraploid wheat species with a spring growth habit

VRN1 genes variability in tetraploid wheat species with a spring growth habit

Wheat gene for all seasons

CaAP2 transcription factor is a candidate gene for a flowering repressor and a candidate for controlling natural variation of flowering time in Capsicum annuum

Contact Info

Product

Resources

About