Molecular characterization of two previously undescribed begomovirus-associated alphasatellite molecules infecting malvaceous species in Cameroon

Leke, W. N.; Kvarnheden, Anders; Avelar, S.; Brown, Judith K.

doi:10.1007/s00705-020-04523-8

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…Alphasatellites infecting malvaceous species have been reported throughout Asia, and also in Burkina Faso, Cameroon, Egypt, Kenya, and Mali [26], and deltasatellites infecting M. coromandelianum plants have been reported in Cuba [5]. However, these DNA satellites were not found in the analyzed samples.…”

Section: Main Textmentioning

confidence: 86%

Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia

Tang

She

et al. 2021

Arch Virol

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Section: Main Textmentioning

confidence: 86%

Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia

Tang

She

et al. 2021

Arch Virol

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“…Scientific reports advised on the potential of weeds as a reservoir or alternative host for BGVs, facilitating their persistence, dissemination to crops of commercial interest, and the emergence of new species ( Jovel et al, 2004 ). Weeds from the Sida and Malvastrum genera are of particular interest because they are reservoirs of several BGVs and natural host of 34 and 10 species, respectively; no other weed genera outnumber these ( Graham, Martin & Roye, 2009 ; Wyant et al, 2011 ; Fiallo-Olivé et al, 2012 ; Tavares et al, 2012 ; Ferro et al, 2017 ; Leke et al, 2020 ). The suitability for BGVs infection shown by Sida and Malvastrum, as well as the ability to harbor BGVs from different species for long periods, could facilitate the co-infection by BGVs, the emergence of new species of begomoviruses arising from recombination, and the spread of BGVs to commercial crops ( Jovel et al, 2004 ; Graham, Martin & Roye, 2009 ; Tavares et al, 2012 ; Ferro et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Sida chlorotic leaf virus: a new recombinant begomovirus found in non-cultivated plants and Cucumis sativus L

García-Rodríguez

Partida-Palacios

Regla-Márquez³

et al. 2023

PeerJ

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Background Begomoviruses are circular single-stranded DNA plant viruses that cause economic losses worldwide. Weeds have been pointed out as reservoirs for many begomoviruses species, especially from members of the Sida and Malvastrum genera. These weeds have the ability to host multiple begomoviruses species simultaneously, which can lead to the emergence of new viral species that can spread to commercial crops. Additionally, begomoviruses have a natural tendency to recombine, resulting in the emergence of new variants and species. Methods To explore the begomoviruses biodiversity in weeds from genera Sida and Malvastrum in Colima, México, we collected symptomatic plants from these genera throughout the state. To identify BGVs infecting weeds, we performed circular DNA genomics (circomics) using the Illumina platform. Contig annotation was conducted with the BLASTn tool using the GenBank nucleotide “nr” database. We corroborated by PCR the presence of begomoviruses in weeds samples and isolated and sequenced the complete genome of a probable new species of begomovirus using the Sanger method. The demarcation process for new species determination followed the International Committee on Taxonomy of Viruses criteria. Phylogenetic and recombination analyses were implemented to infer the evolutionary relationship of the new virus. Results We identified a new begomovirus species from sida and malvastrum plants that has the ability to infect Cucumis sativus L. According to our findings, the novel species Sida chlorotic leaf virus is the result of a recombination event between one member of the group known as the Squash leaf curl virus (SLCV) clade and another from the Abutilon mosaic virus (AbMV) clade. Additionally, we isolated three previously identified begomoviruses species, two of which infected commercial crops: okra (Okra yellow mosaic Mexico virus) and cucumber (Cucumber chlorotic leaf virus). Conclusion These findings support the idea that weeds act as begomovirus reservoirs and play essential roles in begomovirus biodiversity. Therefore, controlling their populations near commercial crops must be considered in order to avoid the harmful effects of these phytopathogens and thus increase agricultural efficiency, ensuring food and nutritional security.

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“…Of late, cotton leaf curl Gezira virus (CLCuGeV) has been associated with OLCD in Burkina Faso, Cameroon, Niger and Sudan [4,[6][7][8][9][10], Okra leaf curl Cameroon virus (OLCuCMV) has so far been associated with OLCD only in Cameroon [4], and okra yellow crinkle virus (OYCrV) has been associated with the disease in Cameroon and Mali [3,4,11]. All three; CLCuGeV, OLCuCMV and OYCrV have been isolated with various associated betasatellites and/or alphasatellites [3,4,[7][8][9][10]12]. With the identi cation of OLCuCMV and new alphasatelites in Burkina Faso and Cameroon [12], the observation of unusual symptoms on okra (Fig 1), the importance of deep sequencing in pathogens identi cation and discovery and a heavy infestation with white ies; the insect vector for Begomoviruses, the objective of this study was to identify and characterize the suspect begomovirus (es) and the associated satDNAs, if present associated with the unusual OLCD observed in Ekona , in Fako Division, in Southwestern Cameroon.…”

Section: Full Textmentioning

confidence: 99%

“…All three; CLCuGeV, OLCuCMV and OYCrV have been isolated with various associated betasatellites and/or alphasatellites [3,4,[7][8][9][10]12]. With the identi cation of OLCuCMV and new alphasatelites in Burkina Faso and Cameroon [12], the observation of unusual symptoms on okra (Fig 1), the importance of deep sequencing in pathogens identi cation and discovery and a heavy infestation with white ies; the insect vector for Begomoviruses, the objective of this study was to identify and characterize the suspect begomovirus (es) and the associated satDNAs, if present associated with the unusual OLCD observed in Ekona , in Fako Division, in Southwestern Cameroon.…”

Section: Full Textmentioning

confidence: 99%

Annotated sequence First report of Soybean chlorotic blotch virus infecting Okra (Abelmoschus esculentus) in Cameroon

Lauryn

Piau

Monono

et al. 2023

Preprint

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Leaves from okra (Abelmoschus esculentus) plants exhibiting unusual viral disease symptoms were tested for the presence of begomovirus infection. The putative full-length Soybean chlorotic blotch virus (SbCBV) components A and B molecules, respectively were amplified, sequenced, and analyzed. This is the first study identifying SbCBV, a bipartite begomovirus infecting okra in Cameroon and the centra Central African region.

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Molecular characterization of two previously undescribed begomovirus-associated alphasatellite molecules infecting malvaceous species in Cameroon

Cited by 4 publications

References 20 publications

Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia

Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia

Sida chlorotic leaf virus: a new recombinant begomovirus found in non-cultivated plants and Cucumis sativus L

Annotated sequence First report of Soybean chlorotic blotch virus infecting Okra (Abelmoschus esculentus) in Cameroon

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