Molecular characterization of two CuZn-superoxide dismutases in a sea anemone

Plantivaux, Amandine; Furla, Paola; Zoccola, Didier; Garello, G.; Forcioli, Didier; Richier, Sophie; Merle, Pierre-Laurent; Tambutté, Éric; Tambutté, Sylvie; Allemand, Denis

doi:10.1016/j.freeradbiomed.2004.06.043

Cited by 56 publications

(36 citation statements)

References 31 publications

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“…Phylogenetic analysis of the N. vectensis SOD genes provides a glimpse into the diversification of SODs early in the evolution of animals and within the cnidarian lineage. Two CuZnSODs have previously been identified in the anemone A. viridis (Plantivaux et al, 2004), one in the hydrozoan H. vulgaris (Dash et al, 2007) and eight from an initial search of the genome of the coral A. digitifera (Shinzato et al, 2012). We have identified three CuZnSODs in N. vectensis, two of which are orthologs of the genes from A. viridis.…”

Section: Discussionmentioning

confidence: 93%

“…NvCuZnSOD3 is orthologous to SODa from A. viridis. Based on the presence of a signal peptide at the N-terminus, SODa is predicted to be extracellular, like the mammalian SOD3 genes (Plantivaux et al, 2004). In N. vectensis the gene prediction of NvCuZnSOD3 appears to be incomplete at the 5′ end, so it is not currently known whether a signal peptide is present.…”

Section: Discussionmentioning

confidence: 99%

“…NvCuZnSOD1 is not recognizable as an ortholog of any previously reported cnidarian SOD. NvCuZnSOD2 is most closely related to SODb from the anemone A. viridis (Plantivaux et al, 2004). NvCuZnSOD3 is positioned within a wellsupported clade that includes SODa from A. viridis as well as SOD3 from human and Xenopus.…”

Section: Diversity Of Sod and Catalase Genes In N Vectensismentioning

confidence: 99%

“…At the sequence level, searches of genomic and expressed sequence tag (EST) databases of the sea anemones Nematostella vectensis (Goldstone, 2008;Reitzel et al, 2008b) and Aiptasia pallida (Sunagawa et al, 2009), the coral Acropora digitifera (Shinzato et al, 2012) and the hydrozoan Hydra magnapapillata (Shinzato et al, 2012) have demonstrated the presence of multiple genes encoding antioxidant enzymes, including CuZnSODs, MnSODs, catalase, thioredoxins and glutathione peroxidases. Two CuZnSODs have been cloned from A. viridis (Plantivaux et al, 2004), and an extracellular CuZnSOD and a mitochondrial MnSOD have been identified in the freshwater hydrozoan Hydra vulgaris (Dash et al, 2007). Catalase has been cloned from A. viridis (Merle et al, 2007) and H. vulgaris (Dash and Phillips, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Activation of the cnidarian oxidative stress response by ultraviolet light, polycyclic aromatic hydrocarbons and crude oil

Tarrant

Reitzel

Kwok

et al. 2014

Journal of Experimental Biology

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Organisms are continuously exposed to reactive chemicals capable of causing oxidative stress and cellular damage. Antioxidant enzymes, such as superoxide dismutases (SODs) and catalases, are present in both prokaryotes and eukaryotes and provide an important means of neutralizing such oxidants. Studies in cnidarians have previously documented the occurrence of antioxidant enzymes (transcript expression, protein expression and/or enzymatic activity), but most of these studies have not been conducted in species with sequenced genomes or included phylogenetic analyses, making it difficult to compare results across species due to uncertainties in the relationships between genes. Through searches of the genome of the sea anemone Nematostella vectensis Stephenson, one catalase gene and six SOD family members were identified, including three copper/zinc-containing SODs (CuZnSODs), two manganesecontaining SODs (MnSODs) and one copper chaperone of SOD (CCS). In 24 h acute toxicity tests, juvenile N. vectensis showed enhanced sensitivity to combinations of ultraviolet radiation (UV) and polycyclic aromatic hydrocarbons (PAHs, specifically pyrene, benzo[a]pyrene and fluoranthene) relative to either stressor alone. Adult N. vectensis exhibited little or no mortality following UV, benzo[a]pyrene or crude oil exposure but exhibited changes in gene expression. Antioxidant enzyme transcripts were both upregulated and downregulated following UV and/or chemical exposure. Expression patterns were most strongly affected by UV exposure but varied between experiments, suggesting that responses vary according to the intensity and duration of exposure. These experiments provide a basis for comparison with other cnidarian taxa and for further studies of the oxidative stress response in N. vectensis.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Diversity Of Sod and Catalase Genes In N Vectensismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Activation of the cnidarian oxidative stress response by ultraviolet light, polycyclic aromatic hydrocarbons and crude oil

Tarrant

Reitzel

Kwok

et al. 2014

Journal of Experimental Biology

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“…Most oxygen-consuming organisms protect themselves from superoxide anion by expressing superoxide dismutases. They prevent the superoxide anion from initiating a radical chain reaction that results in other reactive oxygen species (ROS) provoking damage to DNA, proteins, and membrane lipids (Plantivaux et al 2004;Krutika and Subramanian 2007). SODs are considered as the first and most important line of antioxidant enzyme defense systems by removing the superoxide anion (Igor et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated transformation of SOD gene to Trichoderma harzianum

Yang

Sun

et al. 2009

World J Microbiol Biotechnol

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Trichoderma harzianum is a soil-borne filamentous fungus that exhibits biological control properties because it parasitizes a large variety of phytopathogenic fungi. In this study the SOD gene was successfully transferred into the bio-control fungus Trichoderma harzianum with an efficiency of 60-110 transformants per 10 7 spores by using Agrobacterium tumefaciens-mediated transformation. Putative transformants were analyzed to test the transformation by the southern blot. Antifungal activities of the transformants were examined under abiotic stresses. The transformants were exposed to 40°C for three days and 2 mol/l NaCl at 27°C for 5-10 days to assay antifungal activities with Sclerotinia sclerotiorum. The inhibition rates of the transformants, comparing to Trichoderma harzianum with no SOD gene transferred, were respectively 83.96% after 40°C and 60.13% after 2 mol/l NaCl. The results showed that the SOD transformants had significantly higher resistance to heat and salt stress.

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The past, present, and future of coral heat stress studies

Cziesielski

Schmidt‐Roach

Aranda

2019

Ecology and Evolution

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The global loss and degradation of coral reefs, as a result of intensified frequency and severity of bleaching events, is a major concern. Evidence of heat stress affecting corals through loss of symbionts and consequent coral bleaching was first reported in the 1930s. However, it was not until the 1998 major global bleaching event that the urgency for heat stress studies became internationally recognized. Current efforts focus not only on examining the consequences of heat stress on corals but also on finding strategies to potentially improve thermal tolerance and aid coral reefs survival in future climate scenarios. Although initial studies were limited in comparison with modern technological tools, they provided the foundation for many of today's research methods and hypotheses. Technological advancements are providing new research prospects at a rapid pace. Understanding how coral heat stress studies have evolved is important for the critical assessment of their progress. This review summarizes the development of the field to date and assesses avenues for future research.

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Molecular characterization of two CuZn-superoxide dismutases in a sea anemone

Cited by 56 publications

References 31 publications

Activation of the cnidarian oxidative stress response by ultraviolet light, polycyclic aromatic hydrocarbons and crude oil

Activation of the cnidarian oxidative stress response by ultraviolet light, polycyclic aromatic hydrocarbons and crude oil

Agrobacterium tumefaciens-mediated transformation of SOD gene to Trichoderma harzianum

The past, present, and future of coral heat stress studies

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