Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae

Roberts, Marilyn C.; Elwell, Lynn P.; Falkow, Stanley

doi:10.1128/jb.131.2.557-563.1977

Cited by 134 publications

(72 citation statements)

References 26 publications

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“…The last two penicillin resistant strains (Tor and SBOI) have an additional plasmid of 5.46 kb. The finding of a larger and a smaller beta-lactamase-producing plasmid agrees with previous reports (9,25,28).…”

Section: Resultssupporting

confidence: 93%

“…If one supposes the DdeI/HpaII difference to be the result of one ancient mutational event, one can conclude that both small and large B-plasmids must have entered gonococcal strains more than once; all Ap' gonococcal strains do not originate from the same transformed strain. This conclusion is supported by the finding of different auxotypes, not only between strains harbouring large and small B-plas-mids, but also among strains with the same type of B-plasmid (9,28,8). Before thediscovery ofAp'gonococci, a gonococcal plasmid of about 25 Mdal, later identified as conjugative, was detected (33).…”

Section: Spread Of Gonococcal B-plasmidsmentioning

confidence: 89%

“…Such strains have spread rapidly to an increasing number of countries (26,2,27,9). Penicillin resistance has been shown to be mediated by beta-lactamase-producing (B-) plasmids of approximately 4.7 or 3.3 Mdal (10,28). The larger ones have mainly been isolated from strains originating from Asia, and the smaller ones from Africa and England.…”

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confidence: 99%

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MOLECULAR CHARACTERIZATION AND COMPARISON OF PLASMID CONTENT IN SEVEN DIFFERENT STRAINS OF NEISSERIA GONORRHOEAE

Aalen

Gundersen

1987

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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The plasmid content of one penicillin sensitive and six penicillin resistant strains of Neisseria gonorrhoeae has been examined. All strains harbour a small, phenotypically cryptic plasmid of 4.1 kilo base pairs (kb). Four of the penicillin resistant strains carry a beta‐lactamase‐producing plasmid of 7.3 kb. One of these also carries a large plasmid of about 40 kb. The two remaining penicillin resistant strains harbour a smaller beta‐lactamase‐producing plasmid of 5.5 kb. The plasmids have been subjected to digestion with a number of restriction endonucleases, and their restriction maps have been compared. Judging by the maps, the cryptic (C‐) plasmids show great similarities. Except that two of them have 54 additional basepairs (bp), and two have a Hpall site instead of a Ddel site, no differences were found. The larger beta‐lactamase‐producing (B‐) plasmids have identical maps. The smaller seem to be homologous with the larger, except for a deletion of 1.8 kb. There is no correlation between the variant of C‐plasmid and type of B‐plasmid harboured in the penicillin resistant strains. The evolutionary implications suggested by this finding are discussed.

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Section: Resultssupporting

confidence: 93%

Section: Spread Of Gonococcal B-plasmidsmentioning

confidence: 89%

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MOLECULAR CHARACTERIZATION AND COMPARISON OF PLASMID CONTENT IN SEVEN DIFFERENT STRAINS OF NEISSERIA GONORRHOEAE

Aalen

Gundersen

1987

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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“…Of particular interest is the deletion of TnA in N. gonorrhoeae. The bta plasmids of ampicitlinresistant isolates of N. gonorrhoeae contain a truncated Tr\A insertion (Roberts et at., 1977;Fayet ef at., 1982;Saunders ef at., 1986), This insertion retains approximately 40% of the intact transposon, including the right arm, the bta gene, and part of the regulatory locus. It lacks the left arm, which contains the transposase, the other part of the regulatory locus, and has been shown to be incapable of normal transposition.…”

Section: Discussionmentioning

confidence: 99%

Deletions of Tn916‐like transposons are implicated in tetM‐mediated resistance in pathogenic Neisseria

Swartley

McAllister

Hajjeh

et al. 1993

Molecular Microbiology

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Summary Using the tetM‐containing conjugative transposon Tn916 as a mutagenesis tool, we identified two distinct classes of transposon insertions in the meningococcal chromosome. Class I Insertions have an intact copy of Tn916 that appears to have transposed by a novel recombinational mechanism, similar to the transposition of conjugative transposons in Gram‐positive bacteria. Class II insertions were characterized by deletions of Tn916 but preservation of the tetM determinant. In addition, we identified Class II Tn916‐like insertions in the naturally occurring 25.2 MDa tetM‐containing plasmids of both Neisseria meningitidis and Neisseria gonorrhoeae. The truncated Tn916 like insertions appeared to be present in the same site in these two plasmids; however, the deletions of the transposon were different. Plasmid sequence adjacent to the truncated transposon in the 25.2 MDa plasmids was found in a tetracycline‐sensitive N. gonorrhoeae 24.5 MDa conjugative plasmid. These data suggest that the 25.2 MDa plasmids are the result of one or a series of Class II Tn916‐like insertions into 24.5 MDa conjugative plasmids. Class II insertions of Tn916‐like transposons are implicated in the dissemination of tetM resistance in pathogenic Neisseria.

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“…Bacteria were grown overnight at 37 Њ C in a humidified atmosphere of 5% CO 2 in air on gonococcus (GC) medium agar (Difco) supplemented with Kellogg's supplement solution (0.22 M D-glucose, 0.03 M L -glutamine, 0.001 M ferric nitrate, and 0.02 M cocarboxylase; Sigma-Aldrich) as described previously (29,30). One loopful of meningococci was dissolved in 500 l of PBS and chromosomal DNA was prepared as described previously (31).…”

Section: Methodsmentioning

confidence: 99%

NadA, a Novel Vaccine Candidate of Neisseria meningitidis

Comanducci

Bambini

Brunelli

et al. 2002

The Journal of Experimental Medicine

335

342

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Neisseria meningitidis is a human pathogen, which, in spite of antibiotic therapy, is still a major cause of mortality due to sepsis and meningitis. Here we describe NadA, a novel surface antigen of N. meningitidis that is present in 52 out of 53 strains of hypervirulent lineages electrophoretic types (ET) ET37, ET5, and cluster A4. The gene is absent in the hypervirulent lineage III, in N. gonorrhoeae and in the commensal species N. lactamica and N. cinerea. The guanine/cytosine content, lower than the chromosome, suggests acquisition by horizontal gene transfer and subsequent limited evolution to generate three well-conserved alleles. NadA has a predicted molecular structure strikingly similar to a novel class of adhesins (YadA and UspA2), forms high molecular weight oligomers, and binds to epithelial cells in vitro supporting the hypothesis that NadA is important for host cell interaction. NadA induces strong bactericidal antibodies and is protective in the infant rat model suggesting that this protein may represent a novel antigen for a vaccine able to control meningococcal disease caused by three hypervirulent lineages.

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Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae

Cited by 134 publications

References 26 publications

MOLECULAR CHARACTERIZATION AND COMPARISON OF PLASMID CONTENT IN SEVEN DIFFERENT STRAINS OF NEISSERIA GONORRHOEAE

MOLECULAR CHARACTERIZATION AND COMPARISON OF PLASMID CONTENT IN SEVEN DIFFERENT STRAINS OF NEISSERIA GONORRHOEAE

Deletions of Tn916‐like transposons are implicated in tetM‐mediated resistance in pathogenic Neisseria

NadA, a Novel Vaccine Candidate of Neisseria meningitidis

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