Molecular characterization of three toll-like receptors (TLR21, TLR22, and TLR25) from a primitive ray-finned fish Dabry's sturgeon (Acipenser dabryanus)

Qi, Zhitao; Wang, Sisi; Zhu, Xiao-Zhen; Yang, Yutu; Han, Panpan; Zhang, Qihuan; Zhang, Shuhuan; Shao, Rong; Xu, Qiaoqing; Wei, Qiwei

doi:10.1016/j.fsi.2018.08.033

Cited by 25 publications

(15 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In teleosts, TLR5 might be a potent LPS recognition receptor, as shown by A. hydrophila and Escherichia coli stimulations (Li et al 2020). In A. dabryanus, qPCR revealed upregulation of TLR1-2, TLR4, TLR13, TRL21-22 and TLR25 in head kidney leucocytes upon LPS stimulation, suggesting also other TLRs may be involved in LPS recognition (Han et al 2018;Qi et al 2018;Tang et al 2020), although, except for TLR4, recognition of other TLRs has not been shown in Teleostei or Acipenseriformes. Vertebrate TLR5 recognizes bacterial flagellin, triggering the MyD88-dependent signalling pathway, which activates nuclear factor jB (NF-jB) to stimulate transcription of pro-inflammatory genes, such as interleukin 6 (IL-6) and tumour necrosis factor-a (TNF-a; Pietretti & Wiegertjes 2014;Hwang et al 2010).…”

Section: Pattern Recognition Receptors (Prrs)mentioning

confidence: 96%

“…In P. spathula,He et al (2019) identified 26 TLR homologs, including duplicated genes and pseudogenes: TLR1-5, TLR7-9, TLR13/22, TLR14/18, TLR21, TLR25, TR27 and one unknown TLR from the TLR1 subfamily. Seven TLRs were in-depth characterized in A. dabryanus, that is TLR1 and TLR4 (Han et al 2018), TLR21-22 and TLR25(Qi et al 2018), and TLR2 and TLR13(Tang et al 2020). TLR4 of amniotes recognizes bacterial lipopolysaccharides (LPSs).…”

mentioning

confidence: 99%

See 1 more Smart Citation

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Höhne

Prokopov

Kuhl

et al. 2021

Reviews in Aquaculture

View full text Add to dashboard Cite

Sturgeon immunity is relevant for basic evolutionary and applied research, including caviar-and meat-producing aquaculture, protection of wild sturgeons and their re-introduction through conservation aquaculture. Starting from a comprehensive overview of immune organs, we discuss pathways of innate and adaptive immune systems in a vertebrate phylogenetic and genomic context. The thymus as a key organ of adaptive immunity in sturgeons requires future molecular studies. Likewise, data on immune functions of sturgeon-specific pericardial and meningeal tissues are largely missing. Integrating immunological and endocrine functions, the sturgeon head kidney resembles that of teleosts. Recently identified pattern recognition receptors in sturgeon require research on downstream regulation. We review first acipenseriform data on Toll-like receptors (TLRs), type I transmembrane glycoproteins expressed in membranes and endosomes, initiating inflammation and host defence by molecular pattern-induced activation. Retinoic acid-inducible gene-I-like (RIG-like) receptors of sturgeons present RNA and key sensors of virus infections in most cell types. Sturgeons and teleosts share major components of the adaptive immune system, including B cells, immunoglobulins, major histocompatibility complex and the adaptive cellular response by T cells. The ontogeny of the sturgeon innate and onset of adaptive immune genes in different organs remain understudied. In a genomics perspective, our new data on 100 key immune genes exemplify a multitude of evolutionary trajectories after the sturgeon-specific genome duplication, where some single-copy genes contrast with many duplications, allowing tissue specialization, sub-functionalization or both. Our preliminary conclusion should be tested by future evolutionary bioinformatics, involving all >1000 immunity genes. This knowledge update about the acipenseriform immune system identifies several important research gaps and presents a basis for future applications.

show abstract

Section: Pattern Recognition Receptors (Prrs)mentioning

confidence: 96%

mentioning

confidence: 99%

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Höhne

Prokopov

Kuhl

et al. 2021

Reviews in Aquaculture

View full text Add to dashboard Cite

show abstract

“…Many viruses and bacteria that threaten sturgeons have been identified, including white sturgeon ( Acipenser transmontanus ) iridovirus (WSIV), white sturgeon herpesvirus‐1, 2 (WSHV‐1, 2), shovelnose sturgeon ( Scaphirhynchus platorynchus ) iridovirus (SSIV) (Hua, ) and (Zhang, Liu et al, ; Zhang, Huang et al, ). Although interferon and its induced genes had been studied (Luo, Di et al, ; Luo, Zhang et al, ; Qi et al, ; Xu et al, ), there has been no systematic study of antivirus protein genes in sturgeons. Therefore, a study of Mx and Viperin are urgently required.…”

Section: Introductionmentioning

confidence: 99%

Gene characterization and expression pattern of Mx and Viperin genes in Dabry’s sturgeonAcipenser dabryanus

Zhang

Deng

et al. 2019

J Appl Ichthyol

Self Cite

View full text Add to dashboard Cite

Mx and Viperin are important interferon‐stimulated genes that mediate the antiviral immune response. In this study, we cloned the Mx and viperin genes from Dabry's sturgeon (Acipenser dabryanus). The Mx cDNA sequence contained an open reading frame (ORF) of 1,449 nucleotides, encoding a putative protein of 392 aa, which is significantly shorter than other animal Mx proteins. Although the similarity and identity were low between sturgeon Mx and other animal Mx proteins, sturgeon Mx contains the conserved tripartite GTP binding motif and a dynamin family signature. The sturgeon Mx gene contains eight exons split by seven introns. The sturgeon viperin cDNA sequence contained an ORF of 1,047 bp encoding a putative protein of 349 aa, which is relatively well conserved among species. Sturgeon viperin proteins show 82% similarity with those of Xiphophorus maculatus platyfish and Poecilia formosa Amazon molly. The sturgeon viperin gene has a six exon/five intron structure with the same size of second, third, fourth, and fifth exons between different species. The expression of Mx and viperin was detectable in all tissues examined, with the highest expression in skin for Mx and in peripheral blood for viperin. After mock infection using polyinosine‐polycytidylic acid, Mx and viperin showed significantly upregulated expression in primary spleen leukocytes from 3 hr to 72 hr. Lipopolysaccharide could also induce their expression. These results suggested Mx and Viperin could play a vital antiviral role in the innate immune system of Dabry's sturgeon.

show abstract

“…Similarly, the massively expanded TLR22 and TLR23 families warrant an exploration of their ligand binding properties. TLR22 is upregulated in channel catfish (Ictalurus punctatus) upon bacterial infection [97] and both receptors have been suggested to recognize nucleic acid ligands [98], but some also react to protein or lipid pathogen-associated patterns [167][168][169]. Their role in fish is currently unclear.…”

Section: Gene Expansionsmentioning

confidence: 99%

The round goby genome provides insights into mechanisms that may facilitate biological invasions

et al. 2020

View full text Add to dashboard Cite

Background: The invasive benthic round goby (Neogobius melanostomus) is the most successful temperate invasive fish and has spread in aquatic ecosystems on both sides of the Atlantic. Invasive species constitute powerful in situ experimental systems to study fast adaptation and directional selection on short ecological timescales and present promising case studies to understand factors involved the impressive ability of some species to colonize novel environments. We seize the unique opportunity presented by the round goby invasion to study genomic substrates potentially involved in colonization success. Results: We report a highly contiguous long-read-based genome and analyze gene families that we hypothesize to relate to the ability of these fish to deal with novel environments. The analyses provide novel insights from the large evolutionary scale to the small species-specific scale. We describe expansions in specific cytochrome P450 enzymes, a remarkably diverse innate immune system, an ancient duplication in red light vision accompanied by red skin fluorescence, evolutionary patterns of epigenetic regulators, and the presence of osmoregulatory genes that may have contributed to the round goby's capacity to invade cold and salty waters. A recurring theme across all analyzed gene families is gene expansions. Conclusions: The expanded innate immune system of round goby may potentially contribute to its ability to colonize novel areas. Since other gene families also feature copy number expansions in the round goby, and since other Gobiidae also feature fascinating environmental adaptations and are excellent colonizers, further long-read genome approaches across the goby family may reveal whether gene copy number expansions are more generally related to the ability to conquer new habitats in Gobiidae or in fish.

show abstract

Molecular characterization of three toll-like receptors (TLR21, TLR22, and TLR25) from a primitive ray-finned fish Dabry's sturgeon (Acipenser dabryanus)

Cited by 25 publications

References 42 publications

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Gene characterization and expression pattern of Mx and Viperin genes in Dabry’s sturgeonAcipenser dabryanus

The round goby genome provides insights into mechanisms that may facilitate biological invasions

Contact Info

Product

Resources

About