Molecular characterization of the zebrafish PEA3 ETS-domain transcription factor

Brown, Louise A.; Amores, Angel; Schilling, Thomas F.; Jowett, Trevor; Baert, Jean-Luc; Launoit, Yvan de; Sharrocks, Andrew D

doi:10.1038/sj.onc.1201911

Cited by 55 publications

(47 citation statements)

References 28 publications

(50 reference statements)

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“…In comparison to Elk-1 and SAP-1, the relative binding e ciencies of zTCF-1 closely mirrors those exhibited by Elk-1 (Figure 4b). SAP-1 exhibits a lower siteselectivity and binds all the tested sites better than either zTCF-1 or Elk-1 (Figure 4b; Brown et al, 1998a). The DNA binding speci®city of zTCF-1 is therefore most similar to that of human Elk-1 (see Discussion).…”

Section: Dna Binding Properties Of Ztcf-1mentioning

confidence: 85%

“…Autonomous binding is mediated by the ETS-domain alone and the binding speci®city of zTCF-1 closely resembles that of Elk-1 which exhibits a more stringent binding speci®city than either SAP-1 or SAP-2 (Figure 4, Shore et al, 1996;Brown et al, 1998a). It was previously demonstrated that the presence of amino acid D69 in the Elk-1 ETS-domain (V68 in SAP-1) is mainly responsible for generating this stringent binding speci®city (Shore et al, 1996).…”

Section: Dna Binding Properties Of Ztcf-1mentioning

confidence: 99%

“…zTCF-1 was therefore tested as a potential substrate for representative members of (Brown et al, 1998a). Data are quanti®ed relative to the binding of each protein to the S27 site (taken as 100%).…”

Section: Dna Binding Properties Of Ztcf-1mentioning

confidence: 99%

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Molecular characterization of a zebrafish TCF ETS-domain transcription factor

et al. 1999

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The ternary complex factor (TCF) subfamily of ETStranscription factors represent key nuclear targets of the MAP kinase pathways. Members of this subfamily are classi®ed by the presence of several conserved domains for DNA binding, interaction with SRF, interaction with MAP kinases and transcriptional activation. In this study we have isolated a further member of this subfamily (TCF-1) from zebra®sh. The protein product of zebra®sh TCF-1 (zTCF-1), shares sequence similarity with the mammalian TCFs in all four conserved domains, with highest overall similarity to SAP-1. Zebra®sh TCF-1 is expressed throughout zebra®sh embryonic development and exhibits typical TCF DNA binding characteristics, with the B-box being required for interaction with SRF. Of the mammalian TCFs, its DNA binding speci®city resembles Elk-1. zTCF-1 is a target for both the growth factor/mitogen-activated and stress-activated MAP kinase cascades in vitro and in vivo. However, di erential targeting occurs, with the pro®le of its activation closely resembling that of mammalian SAP-1. Together, our results demonstrate that the TCFs have been functionally conserved during vertebrate development.

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Section: Dna Binding Properties Of Ztcf-1mentioning

confidence: 85%

Section: Dna Binding Properties Of Ztcf-1mentioning

confidence: 99%

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Molecular characterization of a zebrafish TCF ETS-domain transcription factor

et al. 1999

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“…Expression of scyba, which codes for a CXC-type chemokine, is also strongly expressed in the otic placodes from 12 hpf (Long et al, 2000). Transcripts for the ETS-domain transcription factors erm and pea3, and the FGF antagonist sprouty4, mark the otic placodes from early stages and appear to be direct transcriptional targets of FGF-mediated induction (Brown et al, 1998;Mü nchberg et al, 1999;Fü rthauer et al, 2001;Raible and Brand, 2001;Roehl and Nü sslein-Volhard, 2001; see the Role of FGF Signaling section). Expression of these genes overlaps with that of pax2.1 (Raible and Brand, 2001).…”

Section: Placode Inductionmentioning

confidence: 99%

Development of the zebrafish inner ear

Whitfield

Riley

Chiang

et al. 2002

Developmental Dynamics

211

186

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Recent years have seen a renaissance of investigation into the mechanisms of inner ear development. Genetic analysis of zebrafish has contributed significantly to this endeavour, with several dramatic advances reported over the past year or two. Here, we review the major findings from recent work in zebrafish. Several cellular and molecular mechanisms have been elucidated, including the signaling pathways controlling induction of the otic placode, morphogenesis and patterning of the otic vesicle, and elaboration of functional attributes of inner ear.

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“…To compare the two isoforms further we also performed co-transfection experiments in rabbit kidney RK13 cells in the presence of a luciferase reporter gene driven by the three times E74 Ets motifs. Taking into account the fact that transcriptional activation by PEA3 group members is enhanced by the PKA signaling pathway Brown et al, 1998), the involvement of this pathway in regulating transcripis represented by the large box, which contains the acidic (hatched box) and the ETS (black box) domains; the¯anking 5'-and 3'-untranslated regions are shown as small boxes. The acidic and the ETS domains are each composed of three di erent exons.…”

Section: Comparison Of the Binding And Transcriptional Capacities Of mentioning

confidence: 99%

Characterization of the human and mouse ETV1/ER81 transcription factor genes: role of the two alternatively spliced isoforms in the human

Coutte

Monté

Imai³

et al. 1999

Oncogene

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The Ets transcription factors of the PEA3 group ± E1AF/PEA3, ETV1/ER81 and ERM ± are almost identical in the ETS DNA-binding and the transcriptional acidic domains. To accelerate our understanding of the molecular basis of putative diseases linked to ETV1 such as Ewing's sarcoma we characterized the human ETV1 and the mouse ER81 genes. We showed that these genes are both encoded by 13 exons in more than 90 kbp genomic DNA, and that the classical acceptor and donor splicing sites are present in each junction except for the 5' donor site of intron 9 where GT is replaced by TT. The genomic organization of the ETS and acidic domains in the human ETV1 and mouse ER81 (localized to chromosome 12) genes is similar to that observed in human ERM and human E1AF/PEA3 genes. Moreover, as in human ERM and human E1AF/ PEA3 genes, a ®rst untranslated exon is upstream from the ®rst methionine, and the mouse ER81 gene transcription is regulated by a 1.8 kbp of genomic DNA upstream from this exon. In human, the alternative splicing of the ETV1 gene leads to the presence (ETV1a) or the absence (ETV1b) of exon 5 encoding the Cterminal part of the transcriptional acidic domain, but without a ecting the alpha helix previously described as crucial for transactivation. We demonstrated here that the truncated isoform (human ETV1b) and the fulllength isoform (human ETV1a) bind similarly speci®c DNA Ets binding sites. Moreover, they both activate transcription similarly through the PKA-transduction pathway, so suggesting that this alternative splicing is not crucial for the function of this protein as a transcription factor. The comparison of human ETV1a and human ETV1b expression in the same tissues, such as the adrenal gland or the bladder, showed no clear-cut di erences. Altogether, these data open a new avenue of investigation leading to a better understanding of the functional role of this transcription factor.

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Molecular characterization of the zebrafish PEA3 ETS-domain transcription factor

Cited by 55 publications

References 28 publications

Molecular characterization of a zebrafish TCF ETS-domain transcription factor

Molecular characterization of a zebrafish TCF ETS-domain transcription factor

Development of the zebrafish inner ear

Characterization of the human and mouse ETV1/ER81 transcription factor genes: role of the two alternatively spliced isoforms in the human

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