Molecular characterization of the viral structural gene of the first dengue virus type 1 outbreak in Xishuangbanna, a border area of China, Burma and Laos

Abstract

25In the context of recent arbovirus epidemics, dengue fever is becoming a greater concern around the 26 world. In August 2017, Xishuangbanna, which is a border city of China, Burma and Laos, had its 27 first major dengue outbreak. A total of 156 serum samples from febrile patients were collected; 97 28 DENV positive serum samples were screened out, and viral RNAs were successfully and directly 29 extracted, including 77 cases from China and 20 cases from Myanmar. Phylogenetic analysis 30 revealed that all of the strains were classified as DENV-1. There are eight epidemic dengue strains 31 from Myanmar and 74 from Jinghong, Xishuangbanna, that were classified as cluster 1, which are 32 the most similar to the strain of China Guangzhou 2011. There are three epidemic strains from 33 Xishuangbanna Mengla that were classified as cluster 2, which have the closest relationship to the 34 strain of China Hubei 2014. However, there are 12 epidemic strains from Myanmar that were 35 classified as cluster 3, which have the closest relationship to the strain of Laos from 2008, which 36 shows that there is a recycling epidemic trend of DENV in China. There were 236 mutations in the 37 base, which caused 31 nonsynonymous mutations in the DENV structural protein C/prM/E genes 38 when the strain of Xishuangbanna and Myanmar were compared with the DENV-1SS. There is no

Central Nervous System Involvement in Dengue Virus Type 1 Infection: A Pediatric Case Series

“…Genomic sequencing is another tool to help us understand dengue, while specific genotypes of DENV-1 were related to severe dengue outbreaks. 17 18 19…”

The whole‐genome sequencing of prevalent DENV‐1 strains during the largest dengue virus outbreak in Xishuangbanna Dai autonomous prefecture in 2019

“…From 2013 to 2017, new cases and small-scale regional outbreaks occurred almost every year in XDAP. [5][6][7][8] In 2013, 2015, and 2017, 1262, 9 1131, 5 and 1184 10 confirmed DENV cases were reported, respectively. A DENV-1 epidemic occurred from October to December 2018 in XDAP.…”

Detection of Multiple Dengue Infections by Rt-qPCR in West Sumatera, Indonesia

“…Infected human is the main carrier and spreader of DENV, the virus then is transmitted to uninfected mosquitoes for subsequent transmission. Dengue fever is one of the major human infectious diseases in tropical and subtropical areas [8], [9], [10].…”

“…Every year occurs the outbreak of dengue in several provinces with case fatality rate around 0.86-0.89%. Concurrent infection of multiple dengue serotypes has been reported in Indonesia, Mexico, and Puerto Rico as well as other countries [10], [11], [12], [13]. In 2010, Indonesia became the first ranked country in the Association of Southeast Asian Nations by the highest number cases of dengue hemorrhagic fever (DHF) and was predicated as a hyperendemic area because multiple serotypes were found in blood circulation of dengue patients [11], [14].…”