Molecular characterization of the spatial diversity and novel lineages of mycoplankton in Hawaiian coastal waters

Gao, Zheng; Johnson, Zackary I.; Wang, Guangyi

doi:10.1038/ismej.2009.87

Cited by 85 publications

(97 citation statements)

References 57 publications

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“…As with our own study, Gao et al (2008) found a high diversity of Basidiomycete sequences within the Malasseziales, Corticiales, Agaricales and Polyporales. In another study of planktonic marine Coral-associated marine fungi AS Amend et al fungi (Gao et al, 2009), clone libraries were dominated by sequences within the Pucciniomycetes, a group relatively underrepresented here.…”

Section: Discussionmentioning

confidence: 77%

Coral-associated marine fungi form novel lineages and heterogeneous assemblages

Amend

Barshis²,

Oliver³

2011

The ISME Journal

139

100

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Coral stress tolerance is intricately tied to the animal's association with microbial symbionts. The most well-known of these symbioses is that between corals and their dinoflagellate photobionts (Symbiodinium spp.), whose genotype indirectly affects whether a coral can survive cyclical and anthropogenic warming events. Fungi comprise a lesser-known coral symbiotic community whose taxonomy, stability and function is largely un-examined. To assess how fungal communities inside a coral host correlate with water temperature and the genotype of co-occurring Symbiodinium, we sampled Acropora hyacinthus coral colonies from adjacent natural pools with different water temperatures and Symbiodinium identities. Phylogenetic analysis of coral-associated fungal ribosomal DNA amplicons showed a high diversity of Basidiomycetes and Ascomycetes, including several clades separated from known fungal taxa by long and well-supported branches. Community similarity did not correlate with any measured variables, and total fungal community composition was highly variable among A. hyacinthus coral colonies. Colonies in the warmer pool contained more phylogenetically diverse fungal communities than the colder pool and contained statistically significant 'indicator' species. Four taxa were present in all coral colonies sampled, and may represent obligate associates. Messenger RNA sequenced from a subset of these same colonies contained an abundance of transcripts involved in metabolism of complex biological molecules. Coincidence between the taxonomic diversity found in the DNA and RNA analysis indicates a metabolically active and diverse resident marine fungal community.

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Section: Discussionmentioning

confidence: 77%

Coral-associated marine fungi form novel lineages and heterogeneous assemblages

Amend

Barshis²,

Oliver³

2011

The ISME Journal

139

100

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“…Riverine inputs of fungi to coastal waters may to some extent account for the observations made of the biogeographical distribution of mycoplankton in the Pacific Ocean that have shown that diversity is greater in coastal waters around the Hawaiian Islands compared with the open ocean (Gao et al, 2010;Wang et al, 2014), and that Ascomycota in particular are especially more abundant nearer the coast (Wang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…So far, studies of marine mycoplankton diversity have been largely based on samples collected from one-off sampling campaigns that have provided important and insightful 'snap shots' of diversity, but with limited temporal resolution (Bass et al, 2007;Gao et al, 2010;Richards et al, 2012;Manohar and Raghukumar, 2013;. Time series assessments of other plankton groups have proven invaluable in developing our understanding of their biology and ecology (Karl and Church, 2014;Fuhrman et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Here we present a multi-year time series assessment of mycoplankton diversity and abundance, correlating observed changes with cooccurring environmental parameters. We have focussed on the mycoplankton communities at Station L4 because previous studies have indicated that mycoplankton diversity is greater in coastal waters than in the open ocean (Gao et al, 2010;Wang et al, 2014), and the station has been proven to be an exemplar site for elucidating the ecology and biology of other plankton groups Widdicombe et al, 2010;Gilbert et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Multi-year assessment of coastal planktonic fungi reveals environmental drivers of diversity and abundance

2016

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Mycoplankton have so far been a neglected component of pelagic marine ecosystems, having been poorly studied relative to other plankton groups. Currently, there is a lack of understanding of how mycoplankton diversity changes through time, and the identity of controlling environmental drivers. Using Fungi-specific high-throughput sequencing and quantitative PCR analysis of plankton DNA samples collected over 6 years from the coastal biodiversity time series site Station L4 situated off Plymouth (UK), we have assessed changes in the temporal variability of mycoplankton diversity and abundance in relation to co-occurring environmental variables. Mycoplankton diversity at Station L4 was dominated by Ascomycota, Basidiomycota and Chytridiomycota, with several orders within these phyla frequently abundant and dominant in multiple years. Repeating interannual mycoplankton blooms were linked to potential controlling environmental drivers, including nitrogen availability and temperature. Specific relationships between mycoplankton and other plankton groups were also identified, with seasonal chytrid blooms matching diatom blooms in consecutive years. Mycoplankton α-diversity was greatest during periods of reduced salinity at Station L4, indicative of riverine input to the ecosystem. Mycoplankton abundance also increased during periods of reduced salinity, and when potential substrate availability was increased, including particulate organic matter. This study has identified possible controlling environmental drivers of mycoplankton diversity and abundance in a coastal sea ecosystem, and therefore sheds new light on the biology and ecology of an enigmatic marine plankton group. Mycoplankton have several potential functional roles, including saprotrophs and parasites, that should now be considered within the consensus view of pelagic ecosystem functioning and services.

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“…Yet, to our knowledge, no detailed investigations of the response of marine yeast communities to experimental confinement exist and estimates on the percentage of culturable marine yeasts are lacking (Fell 2001). Recently, a DGGE analysis of mycoplankton of Hawaiian coastal waters revealed many new fungal phylotypes but not a single match with fungi previously cultured from this environment (Gao et al 2010). Therefore, future studies on ocean acidification effects on marine yeasts should include direct detection methods.…”

Section: Yeast Community At Helgoland Roadsmentioning

confidence: 99%

Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

et al. 2013

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Marine yeasts play an important role in biodegradation and nutrient cycling and are often associated with marine flora and fauna. They show maximum growth at pH levels lower than present-day seawater pH. Thus, contrary to many other marine organisms, they may actually profit from ocean acidification. Hence, we conducted a microcosm study, incubating natural seawater from the North Sea at present-day pH (8.10) and two near-future pH levels (7.81 and 7.67). Yeasts were isolated from the initial seawater sample and after 2 and 4 weeks of incubation. Isolates were classified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) and representative isolates were identified by partial sequencing of the large subunit rRNA gene. From the initial seawater sample, we predominantly isolated a yeast-like filamentous fungus related to Aureobasidium pullulans, Cryptococcus sp., Candida sake, and various cold-adapted yeasts. After incubation, we found more different yeast species at near-future pH levels than at present-day pH. Yeasts reacting to low pH were related to Leucosporidium scottii, Rhodotorula mucilaginosa, Cryptococcus sp., and Debaryomyces hansenii. Our results suggest that these yeasts will benefit from seawater pH reductions and give a first indication that the importance of yeasts will increase in a more acidic ocean.

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Molecular characterization of the spatial diversity and novel lineages of mycoplankton in Hawaiian coastal waters

Cited by 85 publications

References 57 publications

Coral-associated marine fungi form novel lineages and heterogeneous assemblages

Coral-associated marine fungi form novel lineages and heterogeneous assemblages

Multi-year assessment of coastal planktonic fungi reveals environmental drivers of diversity and abundance

Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

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