Molecular characterization of the possible regulation of multiple bacteriocin production through a three-component regulatory system in Enterococcus faecium NKR-5-3

Ishibashi, Naoki; Matsumoto, Naho; Pérez, Rodney; Iwatani, Shun; Sugino, Haruki; Zendo, Takeshi; Wilaipun, Pongtep; Leelawatcharamas, Vichien; Nakayama, Jiro; Sonomoto, Kenji

doi:10.1016/j.jbiosc.2020.09.019

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…The predicted mature product of this ORF contains an 11-amino-acid sequence showing 73% identity (100% similarity) to the putative VcnQ1-derived pheromone. E. faecium NKR-5-3 produces multiple bacteriocins: enterocins NKR-5-3A, -B, -C, -D, and -Z (Ent53A, Ent53B, Ent53C, Ent53D, and Ent53Z) ( 55 ). An inducing peptide, Ent53D, has been shown to regulate the transcription of the above-mentioned bacteriocins except for NKR-5-3B ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

“… E. faecium NKR-5-3 produces multiple bacteriocins: enterocins NKR-5-3A, -B, -C, -D, and -Z (Ent53A, Ent53B, Ent53C, Ent53D, and Ent53Z) ( 55 ). An inducing peptide, Ent53D, has been shown to regulate the transcription of the above-mentioned bacteriocins except for NKR-5-3B ( 55 ). A derivative of the unannotated ORF in the E. faecium NKR-5-3 genome may be involved in the regulation of NKR-5-3B.…”

Section: Discussionmentioning

confidence: 99%

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Identification of a Novel Two-Peptide Lantibiotic from Vagococcus fluvialis

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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et al. 2022

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Multiple bacteriocin production in lactic acid bacteria

Pérez

Zendo

Sonomoto

2022

Journal of Bioscience and Bioengineering

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Targeting Enterococci with Antimicrobial Activity against Clostridium perfringens from Poultry

et al. 2023

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Necrotic enteritis (NE), caused by Clostridium perfringens, is an emerging issue in poultry farming. New approaches, other than antibiotics, are necessary to prevent NE development and the emergence of multidrug-resistant bacteria. Enterococci are commensal microorganisms that can produce enterocins, antimicrobial peptides with activities against pathogens, and could be excellent candidates for protective cultures. This study aimed to screen and characterize Enterococcus strains of poultry origin for their inhibitory activity against C. perfringens. In total, 251 Enterococcus strains of poultry origin plus five bacteriocin-producing (BP+) E. durans strains of other origins were screened for antimicrobial activity against the indicator C. perfringens X2967 strain using the “spot on the lawn” method. We detected thirty-two BP+ strains (eleven Enterococcus faecium, nine E. gallinarum, eight E. faecalis, three E. durans, and one E. casseliflavus). We further studied the antimicrobial activity of the supernatants of these 32 BP+ strains using agar well diffusion and microtitration against a collection of 20 C. perfringens strains. Twelve BP+ enterococci that were found to exhibit antimicrobial activity against C. perfringens were characterized using whole genome sequencing. Among these, E. faecium X2893 and X2906 were the most promising candidates for further studies as protective cultures for poultry farming. Both strains belong to the sequence type ST722, harbor the genes encoding for enterocin A and enterocin B, do not possess acquired resistance genes, do not carry plasmids, and present the acm gene, which is implicated in host colonization. Further research is needed to determine the utility of these strains as protective cultures.

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Molecular characterization of the possible regulation of multiple bacteriocin production through a three-component regulatory system in Enterococcus faecium NKR-5-3

Cited by 3 publications

References 33 publications

Identification of a Novel Two-Peptide Lantibiotic from Vagococcus fluvialis

Identification of a Novel Two-Peptide Lantibiotic from Vagococcus fluvialis

Multiple bacteriocin production in lactic acid bacteria

Targeting Enterococci with Antimicrobial Activity against Clostridium perfringens from Poultry

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