Molecular characterization of the major outer membrane protein of Haemophilus somnus

Khan, Shakil Ahmed; Tanaka, Akinori; Ide, Hisahiro; Hoshinoo, Kaori; Hanafusa, Yasuko; Tagawa, Yuichi

doi:10.1016/j.vetmic.2005.01.025

Cited by 8 publications

(15 citation statements)

References 32 publications

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“…We showed that p40 was immunodominant (Corbeil et al , 1991) but that the MOMP was not recognized by bovine convalescent phase serum at dilutions of 1:500–1:1000 or more; the dilutions which we usually use in Western blots (Gogolewski et al ., 1987a, b; Ward et al , 1995). Tagawa and colleagues purified and characterized the MOMP (Tagawa et al ., 1993b, d; Khan et al , 2005), showing it to be similar to porins of other Gram-negative bacteria. Tagawa also studied immunologic aspects of the MOMP in Corbeil's laboratory (Tagawa et al , 2000).…”

Section: Virulence Factorsmentioning

confidence: 92%

Histophilus somnihost–parasite relationships

Corbeil

2007

Anim. Health. Res. Rev.

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Histophilus somni (Haemophilus somnus) is one of the key bacterial pathogens involved in the multifactorial etiology of the Bovine Respiratory Disease Complex. This Gram negative pleomorphic rod also causes bovine septicemia, thrombotic meningencephalitis, myocarditis, arthritis, abortion and infertility, as well as disease in sheep, bison and bighorn sheep. Virulence factors include lipooligosaccharide, immunoglobulin binding proteins (as a surface fibrillar network), a major outer membrane protein (MOMP), other outer membrane proteins (OMPs) and exopolysaccharide. Histamine production, biofilm formation and quorum sensing may also contribute to pathogenesis. Antibodies are very important in protection as shown in passive protection studies. The lack of long-term survival of the organism in macrophages, unlike facultative intracellular bacteria, also suggests that antibodies should be critical in protection. Of the immunoglobulin classes, IgG2 antibodies are most implicated in protection and IgE antibodies in immunopathogenesis. The immunodominant antigen recognized by IgE is the MOMP and by IgG2 is a 40 kDa OMP. Pathogenetic synergy of bovine respiratory syncytial virus (BRSV) and H. somni in calves can be attributed, in part at least, to the higher IgE anti-MOMP antibody responses in dually infected calves. Other antigens are probably involved in stimulating host defense or immunopathology as well.

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Section: Virulence Factorsmentioning

confidence: 92%

Histophilus somnihost–parasite relationships

Corbeil

2007

Anim. Health. Res. Rev.

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“…The whole MOMP gene sequences of 178 isolates determined in this study and those of 22 isolates reported previously ( 21 , 28 ) were translated to amino acid sequences, then aligned using the CLUSTALW algorithm ( http://www.clustal.org/omega/ ). After retranslation of the aligned amino acid sequences into the DNA sequences, phylogenetic analysis and round-robin analysis to estimate the evolutionary divergence among the sequences were performed using MEGA 7 software ( https://www.megasoftware.net/ ) ( 29 ).…”

Section: Methodsmentioning

confidence: 99%

“…The major outer membrane protein (MOMP), the most abundant surface protein of H. somni , has several features of the porin proteins of Gram-negative bacteria, such as the presence of 16 transmembrane β-strands and eight extracellular loops (L1-L8) of variable sequences ( 21 ). In other bacterial species, the MOMP and porin gene sequences have great diversity, which has been useful for genetic typing of bacterial isolates of Pasteurella multocida, Haemophilus influenzae, Neisseria spp., ( 22 – 24 ), and others.…”

Section: Introductionmentioning

confidence: 99%

“…In other bacterial species, the MOMP and porin gene sequences have great diversity, which has been useful for genetic typing of bacterial isolates of Pasteurella multocida, Haemophilus influenzae, Neisseria spp., ( 22 – 24 ), and others. As with MOMP and porins of other bacterial species, MOMP of H. somni also has structural and sequence heterogeneity among isolates ( 11 , 21 ). In fact, Tagawa et al ( 11 ) classified 52 H. somni isolates from diseased and healthy animals into six groups (groups 1, 2, 3a, 3b, 3c, and 4) on the basis of the combination of molecular mass of MOMP and its antigen type as determined by the reaction patterns of five monoclonal antibodies.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, to further elucidate the genetic diversity of H. somni isolates and the association of a specific genetic group with a disease phenotype, 200 H. somni isolates from geographically and chronologically diverse origins were analyzed using two approaches: MOMP gene sequencing and PFGE. As mentioned above, H. somni MOMP has antigenic and size variations ( 6 , 11 ) that are ascribed to the nucleotide sequence diversity of the MOMP genes ( 21 ). Therefore, isolates with similar MOMP gene sequences may form disease-associated clades, as described in a previous study of MOMP grouping ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Predominant Clonal Thromboembolic Meningoencephalitis Group of Histophilus somni Assigned by Major Outer Membrane Protein Gene Sequencing and Pulsed-Field Gel Electrophoresis

Ueno

Teratani²,

Misumi

et al. 2018

Front. Vet. Sci.

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Histophilus somni, a member of the family Pasteurellaceae, causes a variety of diseases, including thromboembolic meningoencephalitis (TEME) and respiratory diseases, which result in considerable economic losses to the cattle and sheep industries. In this study, 132 chronologically diverse isolates from cattle in Japan and 68 isolates from other countries comprising 49 from cattle and 19 from sheep were characterized using major outer membrane protein (MOMP) gene sequence and pulsed-field gel electrophoresis (PFGE) analyses. The H. somni isolates formed nine MOMP genetic clades (clade Ia, Ib, and II–VIII) and 10 PFGE clusters (HS1–HS10). Except for two (1.0%), all isolates fell into one of the nine MOMP genetic clades, while 62 (31.0%) isolates belonged to no PFGE cluster. MOMP genetic clade Ia and PFGE cluster HS1 were the major groups, and all HS1 isolates possessed the clade Ia MOMP gene. Isolates from TEME cases were significantly associated with these major groups (chi-square test, p < 0.0001), as 88.2% of the TEME isolates belonged to MOMP genetic clade Ia and PFGE cluster HS1, which formed the most predominant clonal group. After an inactivated vaccine using an HS1 strain with the clade Ia MOMP gene was introduced in Japan in late 1989, the number of TEME cases and isolates assigned into the clonal group decreased simultaneously. However, the proportions of clade Ia and cluster HS1 isolates from TEME cases remained high after 1990. These results suggest a close association of TEME with PFGE cluster HS1 and MOMP genetic clade Ia, and imply the presence of factors or characteristics commonly possessed by those strains that contribute to the development of TEME.

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