Molecular characterization of the <i>Aedes aegypti</i> odorant receptor gene family

Bohbot, Jonathan D.; Pitts, R. Jason; Kwon, Hyung Wook; Rützler, Michael; Robertson, Hugh M.; Zwiebel, Laurence J.

doi:10.1111/j.1365-2583.2007.00748.x

Cited by 217 publications

(289 citation statements)

References 64 publications

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“…This is not surprising, as three of them belong to the similar order Diptera. Similar observations have been made in earlier studies [27] as also among Drosophila species with comparative genomic approaches [6]. Although S. cerevisiae was found to be farthest in homology, six genes of S. cerevisiae show homology to An.…”

confidence: 66%

Fine-scale evolutionary genetic insights into Anopheles gambiae X-chromosome

Srivastava¹,

Dixit²,

Dash³

et al. 2009

JBiSE

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Understanding the genetic architecture of individual taxa of medical importance is the first step for designing disease preventive strategies. To understand the genetic details and evolutionary perspective of the model malaria vector, Anopheles gambiae and to use the information in other species of local importance, we scanned the published X-chromosome sequence for detail characterization and obtain evolutionary status of different genes. The telocentric X-chromosome contains 106 genes of known functions and 982 novel genes. Majorities of both the known and novel genes are with introns. The known genes are strictly biased towards less number of introns; about half of the total known genes have only one or two introns. The extreme sized (either long or short) genes were found to be most prevalent (58% short and 23% large). Statistically significant positive correlations between gene length and intron length as well as with intron number and intron length were obtained signifying the role of introns in contributing to the overall size of the known genes of X-chromosome in An. gambiae. We compared each individual gene of An. gambiae with 33 other taxa having whole genome sequence information. In general, the mosquito Aedes aegypti was found to be genetically closest and the yeast Saccharomyces cerevisiae as most distant taxa to An. gambiae. Further, only about a quarter of the known genes of X-chromosome were unique to An. gambiae and majorities have orthologs in different taxa. A phylogenetic tree was constructed based on a single gene found to be highly orthologous across all the 34 taxa. Evolutionary relationships among 13 different taxa were inferred which corroborate the previous and present findings on genetic relationships across various taxa.

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confidence: 66%

Fine-scale evolutionary genetic insights into Anopheles gambiae X-chromosome

Srivastava¹,

Dixit²,

Dash³

et al. 2009

JBiSE

View full text Add to dashboard Cite

show abstract

“…Interestingly, the clustered arrangement is also maintained in other insect genomes (Xu et al, 2003;Forêt and Maleszka 2006). Drosophila OR and GR genes, on the other hand, appear more scattered throughout the genome, having only a few clusters (Robertson et al, 2003); this distribution differs from other insects where the receptor genes are arranged in a number of clusters (Robertson and Wanner, 2006;Bohbot et al, 2007;Engsontia et al, 2008). Indeed, the chromosomal distribution of OR genes have revealed repeated inter-chromosomal translocation events across the Drosophila phylogeny; these evolutionary events seem to be more frequent in this receptor family than in the OBP family (Guo and Kim, 2007;Conceição and Aguadé, 2008).…”

Section: Genomic Organisation and Phylogenetic Analysismentioning

confidence: 90%

Molecular evolution of the major chemosensory gene families in insects

2009

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Chemoreception is a crucial biological process that is essential for the survival of animals. In insects, olfaction allows the organism to recognise volatile cues that allow the detection of food, predators and mates, whereas the sense of taste commonly allows the discrimination of soluble stimulants that elicit feeding behaviours and can also initiate innate sexual and reproductive responses. The most important proteins involved in the recognition of chemical cues comprise moderately sized multigene families. These families include odorant-binding proteins (OBPs) and chemosensory proteins (CSPs), which are involved in peripheral olfactory processing, and the chemoreceptor superfamily formed by the olfactory receptor (OR) and gustatory receptor (GR) families. Here, we review some recent evolutionary genomic studies of chemosensory gene families using the data from fully sequenced insect genomes, especially from the 12 newly available Drosophila genomes. Overall, the results clearly support the birth-and-death model as the major mechanism of evolution in these gene families. Namely, new members arise by tandem gene duplication, progressively diverge in sequence and function, and can eventually be lost from the genome by a deletion or pseudogenisation event. Adaptive changes fostered by environmental shifts are also observed in the evolution of chemosensory families in insects and likely involve reproductive, ecological or behavioural traits. Consequently, the current size of these gene families is mainly a result of random gene gain and loss events. This dynamic process may represent a major source of genetic variation, providing opportunities for FUTURE specific adaptations.

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“…As chemoreceptors appear to evolve very rapidly (for example, Robertson and Wanner, 2006), it is not always possible to identify their coding sequences by sequencing them directly using other species' primers. However, at least in insects, chemoreceptors share common molecular features across species that allow the use of sequence and structural homology to identify them in new species genomes as genome sequencing is achieved (for example, Krieger et al, 2004;Bohbot et al, 2007). Thus, more non-model genomes are needed to address the molecular basis of chemosensory speciation and more generally of chemically based behaviours.…”

Section: Future Directionsmentioning

confidence: 99%

On the scent of speciation: the chemosensory system and its role in premating isolation

2008

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Chemosensory speciation is characterized by the evolution of barriers to genetic exchange that involve chemosensory systems and chemical signals. Here, we review some representative studies documenting chemosensory speciation in an attempt to evaluate the importance and the different aspects of the process in nature and to gain insights into the genetic basis and the evolutionary mechanisms of chemosensory trait divergence. Although most studies of chemosensory speciation concern sexual isolation mediated by pheromone divergence, especially in Drosophila and moth species, other chemically based behaviours (habitat choice, pollinator attraction) can also play an important role in speciation and are likely to do so in a wide range of invertebrate and vertebrate species. Adaptive divergence of chemosensory traits in response to factors such as pollinators, hosts and conspecifics commonly drives the evolution of chemical prezygotic barriers. Although the genetic basis of chemosensory speciation remains largely unknown, genomic approaches to chemosensory gene families and to enzymes involved in biosynthetic pathways of signal compounds now provide new opportunities to dissect the genetic basis of these complex traits and of their divergence among taxa.

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Molecular characterization of the Aedes aegypti odorant receptor gene family

Cited by 217 publications

References 64 publications

Fine-scale evolutionary genetic insights into Anopheles gambiae X-chromosome

Fine-scale evolutionary genetic insights into Anopheles gambiae X-chromosome

Molecular evolution of the major chemosensory gene families in insects

On the scent of speciation: the chemosensory system and its role in premating isolation

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