Molecular Characterization of the Growth Phase-Dependent Expression of the
            <i>lsrA</i>
            Gene, Encoding Levansucrase of
            <i>Rahnella aquatilis</i>

Seo, Jeong Woo; Jang, Ki‐Hyo; Kang, Soon Ah; Song, Kyung-Sik; Jang, Eun Kyung; Park, Buem Seek; Kim, Chul Ho; Rhee, Shin Hyung

doi:10.1128/jb.184.21.5862-5870.2002

Cited by 13 publications

(15 citation statements)

References 32 publications

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“…(b) Transcriptional regulation of lsr A expression in Rahnella aquatilis . Arrowheads mean activation, perpendicular arrowheads mean inhibition (modified from Seo et al. 2002).…”

Section: Microbial Fructosyltransferase Gene Expressionmentioning

confidence: 99%

Microbial fructosyltransferases and the role of fructans

Velázquez-Hernández

Baizabal-Aguirre

Bravo-Patiño

et al. 2009

Journal of Applied Microbiology

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Summary Microbial fructosyltransferases are polymerases that are involved in microbial fructan (levan, inulin and fructo‐oligosaccharide) biosynthesis. Structurally, microbial fructosyltransferase proteins share the catalytic domain of glycoside hydrolases 68 family and are grouped in seven phylogenetically related clusters. Fructosyltransferase‐encoding genes are organized in operons or in clusters associated with other genes related to carbohydrate metabolism or fructosyltransferase secretion. Fructosyltransferase gene expression is mainly regulated by two‐component systems or phosphorelay mechanisms that respond to sucrose availability or other environmental signals. Microbial fructans are involved in conferring resistance to environmental stress such as water deprivation, nutrient assimilation, biofilm formation, and as virulence factors in colonization. As a result of the biological and industrial importance of fructans, fructosyltransferases have been the subject of extensive research, conducted to improve their enzymatic activity or to elucidate their biological role in nature.

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“…(b) Transcriptional regulation of lsr A expression in Rahnella aquatilis . Arrowheads mean activation, perpendicular arrowheads mean inhibition (modified from Seo et al. 2002).…”

Section: Microbial Fructosyltransferase Gene Expressionmentioning

confidence: 99%

Microbial fructosyltransferases and the role of fructans

Velázquez-Hernández

Baizabal-Aguirre

Bravo-Patiño

et al. 2009

Journal of Applied Microbiology

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“…Growth phase-dependent transcription of the levansucrase-encoding gene, lsrA, of Rahnella aquatilis provides an example of two antagonistically acting regulatory elements, a cis-acting repressor region, lsrR, and a trans-acting activator protein, LsrS (50). Another common mechanism for posttranslational regulation is the specific proteolysis of transcriptional regulators (16,44).…”

Section: Vol 188 2006mentioning

confidence: 99%

Rem, a New Transcriptional Activator of Motility and Chemotaxis in Sinorhizobium meliloti

et al. 2006

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More than 50 genes of the bacterial genetic reservoir are required for motility and chemotaxis. These genes are strictly regulated by a hierarchy of transcription controls that determine the temporal order of flagellar assembly, motility, and chemotaxis, as has been intensely studied in enterobacteria, such as Escherichia coli and Salmonella enterica serovar Typhimurium (1, 31, 56). The E. coli flagella, motility, chemotaxis, and regulatory genes map in four separate clusters referred to as the flagellar regulon. These have been assigned to three sequentially expressed classes. Class I comprises two genes, flhD and flhC, encoding the global transcriptional activator FlhD 2 FlhC 2 . This, in turn, regulates the expression of class II genes, including determinants of the flagellar basal body, the flagellin-specific type III export, the flagellar hook, and FliA, a 28 ( F ) transcription factor for class III. This ultimate class contains the fla (flagellin), mot (proton channel), and che (signal transduction) genes.The nitrogen-fixing plant symbiont Sinorhizobium meliloti, a member of the ␣ subgroup of proteobacteria (38), differs from the enterobacterial ␥ subgroup behavioral scheme in its filament structure, the mode of flagellar rotation, and steps of signal procession (49). The rigid "complex" flagellar filaments consist of four related flagellin subunits, and interflagellin bonds lock the filaments in right-handedness (7,20,48). Hence, S. meliloti cells are propelled by exclusively clockwiserotating flagella, and swimming cells respond to tactic stimuli by modulating their rotary speed (2, 47). Whereas in E. coli tactic signals are processed by a single response regulator, CheY, and a phosphatase, CheZ, signal processing in S. meliloti involves a retrophosphorylation loop with two response regulators, CheY1 and CheY2, but no phosphatase (49,53,54). In addition, a new periplasmic motor protein, MotC, controls flagellar rotary speed in an as yet enigmatic way (41). The arrangement of chemotaxis (che), flagellar (fla, flg, flh, and fli), motility (mot), and regulatory (visN and visR) genes differs from the enterobacterial pattern in that all 51 known genes are clustered in one contiguous 56-kb chromosomal region, the flagellar regulon (19,55). Two genes, visN and visR (assigned class IA), encode the LuxR-type subunits of a heterodimeric (or heterotetrameric) global transcriptional activator, VisNR (52). Inactivating deletions of visN or visR were shown to result in the loss of class II (flg, flh, fli, and mot) and class III (fla and che) gene expression, suggesting that their transcription is directly controlled by the global regulator VisNR (52). However, while VisNR is synthesized throughout growth, swimming motility is restricted to exponential growth. We describe here another master regulator, Rem (regulator of exponential growth motility) (Smc03046 [19]), that actively controls the transcription of class II genes and that confines their expression to the exponential phase of bacterial growth. The monocistroni...

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“…We have previously demonstrated the growth phasedependent expression of the Rahnella aquatilis levansucrase gene (lsrA) in the pRL1CPR vector in the heterologous host Escherichia coli (Seo et al 2000(Seo et al , 2002. The expression pattern of the lsrA gene resulted from the cooperative function of two genetic elements upstream of lsrA, cis-acting lsrR DNA sequences and trans-acting transcriptional regulator LsrS (Fig.…”

Section: Role Of Promoter Sequences Upstream Of Lsramentioning

confidence: 98%

“…2a), at the similar level (1.35 U/mg protein) to pRL1CPR. Then, we also checked the expression of lsrA on pRL1CP-DNheI in which 0.6-kb DNA region including native promoter sequences upstream of lsrA was removed (Seo et al 2002). LsrA activity was 14.5% lower in pRL1CP-DNheI compared to pRL1CP.…”

Section: Role Of Promoter Sequences Upstream Of Lsramentioning

confidence: 99%

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An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli

et al. 2009

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The levansucrase gene (lsrA) from Rahnella aquatilis was strongly expressed in a constitutive manner in Escherichia coli when cloned into a pBluescript KS-based pRL1CP plasmid vector. The native promoter upstream of lsrA and the lacZ promoter cooperatively enhanced the expression of lsrA to a level that was comparable to that of the T7 promoter, which is used in commercial pET expression vector system. A putative rho-independent transcription termination signal downstream of lsrA was crucial for gene expression. This plasmid vector also proved to be applicable for efficient expression of other foreign genes in E. coli.

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Molecular Characterization of the Growth Phase-Dependent Expression of the lsrA Gene, Encoding Levansucrase of Rahnella aquatilis

Cited by 13 publications

References 32 publications

Microbial fructosyltransferases and the role of fructans

Microbial fructosyltransferases and the role of fructans

Rem, a New Transcriptional Activator of Motility and Chemotaxis in Sinorhizobium meliloti

An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli

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