Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker

Abstract

Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer.

Evolutionary diversification of DYX1C1 transcripts via an HERV-H LTR integration event

“…This result is concordant with a previous report on the expression of the DYX1C1 gene (Kim et al, 2009). DYX1C1 has a role in the migration of neocortical neurons and, more specifi- Fig.…”

“…The four alternative spliced forms (V1-1, V1-2, V1-3, and V1-4) resulted in DYX1C1 either acquiring novel exons or losing original exons. The PCR products of V1-1 and V1-2 showed only a subtle difference in size, though V1-1 contains an extra exon compared to V1-2 (Kim et al, 2009). We also confirmed these alternative forms by sequencing them.…”

“…The standard RT-PCR showed similar expression levels of V2 and V3 transcripts in human tissues. However, we found that the transcriptional activity of V3 was higher than that of V2 in tumor tissue than in normal tissue using semi-quantitative RT-PCR analysis in a previous report (Kim et al, 2009). Based on these results, we suggest that the HERV-H LTR element was inserted into the catarrhine lineage after its divergence from the platyrrhine lineage.…”

A theoretical molecular network for dyslexia: integrating available genetic findings

“…58,59 Apart from its clear role in neuronal migration, several alternatively spliced transcript variants of DYX1C1 appear to be biomarkers for colorectal cancer. 60 In addition, through one of its tetratricopeptide repeat domains, the DYX1C1 protein functions as a cochaperone by interacting with HSP70 (heat shock protein 70) and HSP90 (heat shock protein 90) proteins in breast cancer cells. 61 Finally, in hippocampal neurons, DYX1C1 interacts with both the nuclear and cytoplasmic estrogen receptors a (ESR1) and b (ESR2) 62 (see below).…”

Meeting report: Frontiers in genetics: genomics and epigenomics

“…The expression pattern of the transcripts is different between normal and cancer tissues. Thus, it was suggested that the alternatively spliced transcript variants of DYX1C1 gene could be used as a cancer biomarker (Kim et al 2009). In addition, the Choroideremia (CHM) gene has two transcript variants, CHM isoform a and b. LTR12C element existing in the gene confer an alternative splicing site on the gene which result in CHM isoform b.…”