Molecular characterization of the C-3 DNA puff gene of Rhynchosciara americana

Penalva, Luiz O. F.; Yokosawa, Jonny; Stocker, Ann Jacob; Soares, M. A. M.; Graessmann, M.; Orlando, Tereza Cristina; Winter, Carlos Eduardo; Botella, Luisa María; Graessmann, A.; Lara, Francisco J.

doi:10.1016/s0378-1119(97)00104-2

Cited by 32 publications

(55 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Comparison of DNA puff polypeptide sequences with protein data bases results in no significant matches with other known protein sequences, which places DNA puff proteins in a distinct class of proteins (24). In previous work we have identified the BhB10-1 gene product as a 23-kDa polypeptide in immunoblots of salivary glands from late larvae (6).…”

Section: Discussionmentioning

confidence: 93%

“…If this is the case, one might expect that the elements which activate BhB10-1 transcription in B. hygida last-instar larvae would be located outside the tested fragment (positions -817 bp to +1116 bp, determined in relation to the BhB10-1 gene transcription initiation site (6)). With this in mind, it is interesting to note that the BhB10-1 2-kb fragment contains an AT-rich sequence which is the only feature shared between the 5 flanking sequences of DNA puff genes (24), including the two DNA puff constructs which were expressed in a regulated manner in Drosophila (7,8). Alternatively, the absence of an increase in the steady-state levels of BhB10-1 mRNA during the prepupal stage in transgenic Drosophila could be indicating that the factors involved in the induction of the BhB10-1 gene in B. hygida late larvae are not conserved between B. hygida and Drosophila.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The DNA puff BhB10-1 gene is differentially expressed in various tissues of Bradysia hygida late larvae and constitutively transcribed in transgenic Drosophila

Monesi

Sousa

Paçó-Larson

2001

Braz J Med Biol Res

View full text Add to dashboard Cite

We extended the characterization of the DNA puff BhB10-1 gene of Bradysia hygida by showing that, although its mRNA is detected only at the end of the fourth larval instar, BhB10-1 expression is not restricted to the salivary gland, the tissue in which this gene is amplified. Different amounts of BhB10-1 mRNA were detected in other larval tissues such as gut, Malpighian tubules, fat body, brain and cuticle, suggesting that this gene is expressed differentially in the various tissues analyzed. Analysis of transgenic Drosophila carrying the BhB10-1 transcription unit and flanking sequences revealed that the tested fragment promotes transcription in a constitutive manner. We suggest that either cis-regulatory elements are missing in the transgene or factors that temporally regulate the BhB10-1 gene in B. hygida are not conserved in Drosophila.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

The DNA puff BhB10-1 gene is differentially expressed in various tissues of Bradysia hygida late larvae and constitutively transcribed in transgenic Drosophila

Monesi

Sousa

Paçó-Larson

2001

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…These genomic regions constitute sites of developmentally regulated gene amplification and transcription (Basso et al, 2002;Foulk et al, 2006;Gerbi et al, 1993;Lara et al, 1991;Stocker et al, 1996). The formation of DNA puffs is induced by ecdysone and is concomitant with the transcription of the genes which present the highest levels of amplification within the amplicons (Fontes et al, 1992;Gerbi et al, 1993;Glover et al, 1982;Paçó-Larson et al, 1992;Penalva et al, 1997;Santelli et al, 1991Santelli et al, , 2004Wu et al, 1993;Yokosawa et al, 1999).…”

mentioning

confidence: 99%

“…The BhC4-1 protein is expressed at the time DNA puff C4 is formed and is found both in secretory granules, in the salivary gland, and in fibrous structures present in the saliva of fourth instar larvae (Monesi et al, 2004), a subcellular localization consistent with the proposed role for DNA puff proteins. It is noteworthy that in spite of the availability of complete genome sequences for different insect species, the only identified proteins that present similarity to the BhC4-1 protein are the products of the C8 and B2 DNA puffs of Rhynchosciara americana (Frydman et al, 1993;Penalva et al, 1997;Santelli et al, 2004), strongly suggesting that the BhC4-1 protein is a member of a protein family unique to sciarids.…”

mentioning

confidence: 99%

Functional and bioinformatics analyses reveal conservation of cis‐regulatory elements between sciaridae and drosophilidae

Lecci

Malta

Flausino

et al. 2008

Genesis

View full text Add to dashboard Cite

Summary: The sciarid DNA puff C4 BhC4-1 gene is amplified and transcribed in salivary glands at the end of the larval stage. In transgenic Drosophila, the BhC4-1 promoter drives transcription in prepupal salivary glands and in the ring gland of late embryos. A bioinformatics analysis has identified 162 sequences similar to distinct regions of the BhC4-1 proximal promoter, which are predominantly located either in 5 0 or 3 0 regions or introns in the Drosophila melanogaster genome. A significant number of the identified sequences are found in the regulatory regions of Drosophila genes that are expressed in the salivary gland. Functional assays in Drosophila reveal that the BhC4-1 proximal promoter contains both a 129 bp (2186/258) salivary gland enhancer and a 67 bp

show abstract

“…Durante o período de construção do casulo notam-se amplificação e expansão dos pufes de DNA descritos em R. americana, os pufes da região 2 do cromossomo B (B2), da região 3 do cromossomo C (C3) e da região 8 do cromossomo (C8) e relacionam-se esses pufes a genes codificantes de peptídeos estruturais secretados para a construção do casulo comunal, importante para uma metamorfose bemsucedida (Winter et al, 1977;Penalva et al, 1997;Santelli et al, 2004). Ver figura 3 e 4.…”

Section: Rhynchosciara Esses Cromossomos Eram Pelo Menos 100 Vezes Munclassified

Caracterização dos ciclos replicativos nas células foliculares e nutridoras no ovário de <i>Rhynchosciara americana</i> e perfil de expressão de ciclinas A e B.

Chaves¹

View full text Add to dashboard Cite

Study of the diptera Rhynchosciara americana has provided important information about chromosome biology, for it displays polytene chromosomes and gene amplification in different organs. Among the organs that possess cell cycle specializations, the ovary is one of the most interesting ones, showing unique characteristics, such as the synchronous development of follicles and the presence of a single giant nurse cell connected to the oocyte through a cytoplasmic channel. This organ gathers: meiosis in the oocyte, nurse cell with polyploidy followed by polyteny, and follicle cells in mitosis. Polyploid and polytene cells have extra copies of genomic DNA obtained via sequential cycles of S phase not followed by cell division, a process called endoreplication. Cell cycles are driven by the oscilation in the activation of different cyclin/CDK complexes. Studies have shown that Cyclin A acts in endoreplicating cells in Drosophila and Cyclin B inhibits endoreplicative cycles, inducing cell division. Thus, it is relevant to investigate the association between these cell cycle-regulating proteins and the endoreplication cycles that occur during the oogenesis of R. americana. Expression profiles of cyclins A and B were evaluated in the ovary via RT-PCR throughout the development. Thymidine nucleoside incorporation assays showed high proliferative activity in follicle cells to build the follicle and the end of endoreplicative activity in nurse cells of 4-day-old pupae. Protein immunolocalization in ovary at the stage of pupa has shown accumulation of Cyclin A in the cytoplasm of nurse cells and oocytes, and accumulation of Cyclin B in the cytoplasm and germinal vesicle of the oocyte, acting on meiosis mechanisms. The study of proteins related to cell cycle in this model is important for a better understanding of uncommon cell cycle in different insect organ.

show abstract

Molecular characterization of the C-3 DNA puff gene of Rhynchosciara americana

Cited by 32 publications

References 38 publications

The DNA puff BhB10-1 gene is differentially expressed in various tissues of Bradysia hygida late larvae and constitutively transcribed in transgenic Drosophila

The DNA puff BhB10-1 gene is differentially expressed in various tissues of Bradysia hygida late larvae and constitutively transcribed in transgenic Drosophila

Functional and bioinformatics analyses reveal conservation of cis‐regulatory elements between sciaridae and drosophilidae

Caracterização dos ciclos replicativos nas células foliculares e nutridoras no ovário de <i>Rhynchosciara americana</i> e perfil de expressão de ciclinas A e B.

Contact Info

Product

Resources

About