Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34

Pocard, Jean-Alain; Vincent, Nadine; Boncompagni, Éric; Smith, Linda Tombras; Poggi, Marie-Christine; Rudulier, Daniel Le

doi:10.1099/00221287-143-4-1369

Cited by 71 publications

(60 citation statements)

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“…The luteolin-induced osmotolerance could increase bacterial fitness and thus constitutes an advantage for its successful plant root colonization (Biondi et al 2009). The two major osmoprotection systems characterized in E. meliloti are represented by betaine-glycine (Mandon et al 2003;Le Rudulier et al 1984;Pocard et al 1997) and ectoine (Talibart et al 1994). In vitro expression studies Capela et al 2005;Roux et al 2014) revealed no evidences that the betaine system (betICBA operon) was induced by luteolin.…”

Section: Discussionmentioning

confidence: 99%

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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Background and aims The establishment of a successful symbiosis between the nitrogen-fixing bacterium Ensifer meliloti and compatible host legumes (Medicago spp.) depends on a complex molecular signal exchange. The early stage of signaling involves the release from plant roots of the flavonoid luteolin, which in turn induces the expression of rhizobia nodulation (nod) genes required for root infection and nodule development. To date, the bacterial response to the luteolin perception has been characterized in detail as far as gene expression is concerned. Nevertheless, despite this molecular information, a global view on E. meliloti phenotypes affected by the plant signal luteolin is still lacking.Therefore, an extensive phenotypic investigation of luteolin effect on the nitrogen-fixing E. meliloti 3001 has been performed. Methods A thousand different growth conditions, including sensitivity to osmolites, pH stresses, antibiotics and toxic compounds, were tested by the application of the high-throughput Phenotype MicroArray (PM) technology, as well as by several dedicated assays to evaluate growth stimulation, motility, biofilm formation, Nacyl homoserine lactones and Indole-3-acetic acid (IAA) production. Results Results revealed that the plant signal luteolin affected a wide spectrum of E. meliloti 3001 phenotypes. E. meliloti 3001 displayed an enhanced resistance phenotype in the presence of luteolin toward a broad set of chemicals including several antibiotics, toxic ions, respiration inhibitors, membrane damagers, DNA intercalants and other potential antimicrobial agents. Moreover, the presence of luteolin significantly reduced overall AHLs production, as well as the lag phase in relation to the starting cellular density, the motility and biofilm formation under nutrient-limited growth conditions. An effect on E. meliloti indole-3-acetic acid (IAA) production was also detected in vitro as a response to luteolin. Conclusions Overall, these findings suggest that the plant signal luteolin triggers a broad response in E. meliloti 3001, which was shown to be dependent on nutritional conditions sensed by the bacterium, pointing out a wide role in modifying rhizobial phenotypes, possibly in relation to plant root association and then symbiotic interaction.Plant Soil (2016) 399:159-178

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Section: Discussionmentioning

confidence: 99%

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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“…The aldehyde dehydrogenase superfamily comprises seven enzymes of bacterial and plant origin with known involvement in the synthesis of the osmoprotectant glycine betaine from its precursor glycine betaine aldehyde. These are the BetB proteins from E. coli (13) and Rhizobium meliloti (41) and betaine aldehyde dehydrogenases from plants (Spinacia oleracea, Beta vulgaris, Atriplex hortensis, Sorghum bicolor, and Hordeum vulgare) involved in the cellular adaptation to salinity and drought (24,36,53,56,57). The B. subtilis enzyme exhibits 39% sequence identity to the BetB proteins from E. coli and R. meliloti.…”

Section: Vol 178 1996 Glycine Betaine Synthesis In B Subtilis 5123mentioning

confidence: 99%

Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes

et al. 1996

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Synthesis of the osmoprotectant glycine betaine from the exogenously provided precursor choline or glycine betaine aldehyde confers considerable osmotic stress tolerance to Bacillus subtilis in high-osmolarity media. Using an Escherichia coli mutant (betBA) defective in the glycine betaine synthesis enzymes, we cloned by functional complementation the genes that are required for the synthesis of the osmoprotectant glycine betaine in B. subtilis. The DNA sequence of a 4.1-kb segment from the cloned chromosomal B. subtilis DNA was established, and two genes (gbsA and gbsB) whose products were essential for glycine betaine biosynthesis and osmoprotection were identified. The gbsA and gbsB genes are transcribed in the same direction, are separated by a short intergenic region, and are likely to form an operon. The deduced gbsA gene product exhibits strong sequence identity with members of a superfamily of specialized and nonspecialized aldehyde dehydrogenases. This superfamily comprises glycine betaine aldehyde dehydrogenases from bacteria and plants with known involvement in the cellular adaptation to high-osmolarity stress and drought. The deduced gbsB gene product shows significant similarity to the family of type III alcohol dehydrogenases. B. subtilis mutants with defects in the chromosomal gbsAB genes were constructed by marker replacement, and the growth properties of these mutant strains in high-osmolarity medium were analyzed. Deletion of the gbsAB genes destroyed the cholineglycine betaine synthesis pathway and abolished the ability of B. subtilis to deal effectively with high-osmolarity stress in choline-or glycine betaine aldehyde-containing medium. Uptake of radiolabelled choline was unaltered in the gbsAB mutant strain. The continued intracellular accumulation of choline or glycine betaine aldehyde in a strain lacking the glycine betaine-biosynthetic enzymes strongly interfered with the growth of B. subtilis, even in medium of moderate osmolarity. A single transcription initiation site for gbsAB was detected by high-resolution primer extension analysis. gbsAB transcription was initiated from a promoter with close homology to A -dependent promoters and was stimulated by the presence of choline in the growth medium.

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“…1). The S. meliloti chromosome also encodes a betaine aldehyde dehydrogenase, as well as choline dehydrogenase, BetA (65). The pSymA BetI may be redundant with chromosomal SMC00095 (49).…”

Section: Stress Responsesmentioning

confidence: 99%

Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid

Barnett

Fisher

Jones

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

281

227

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The symbiotic nitrogen-fixing soil bacterium Sinorhizobium meliloti contains three replicons: pSymA, pSymB, and the chromosome. We report here the complete 1,354,226-nt sequence of pSymA. In addition to a large fraction of the genes known to be specifically involved in symbiosis, pSymA contains genes likely to be involved in nitrogen and carbon metabolism, transport, stress, and resistance responses, and other functions that give S. meliloti an advantage in its specialized niche.

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Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34

Cited by 71 publications

References 50 publications

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes

Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid

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