Molecular characterization of the autophagy-related gene Beclin-1 from the olive flounder (Paralichthys olivaceus)

Kong, Hee Jeong; Moon, Ji‐Young; Nam, Bo‐Hye; Kim, Young‐Ok; Kim, Woo Jin; Lee, Jehee; Kim, Kyung-Kil; Kim, Bong-Seok; Yeo, Sang-Yeob; Lee, Chang Hoon; Kang, Kwang-Il; Lee, Sang-Jun

doi:10.1016/j.fsi.2011.05.002

Cited by 23 publications

(8 citation statements)

References 35 publications

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“…An early study showed that overexpressing Beclin-1 of olive flounder (Paralichthys olivaceus) decreased VHSV G transcripts compared to control cells. 129 Besides, SVCV replication on EPC cells was inhibited after interfering with LC3 or Beclin1, which is consistent with the effect of autophagy inhibitors on SVCV. 36 ATG5, ATG12 and ATG5-ATG12 complexes of grass carp (Ctenopharyngodon idella) can down-regulate the transcriptional expression levels of the interferon (IFN) signalling pathway in response to ploy I:C or GCRV.…”

Section: Crosstalk Between Autophagy and Immune Response During Aquat...supporting

confidence: 74%

“…In response to virus infection, the autophagic roles of some ATG proteins are dominant. An early study showed that overexpressing Beclin‐1 of olive flounder ( Paralichthys olivaceus ) decreased VHSV G transcripts compared to control cells 129 . Besides, SVCV replication on EPC cells was inhibited after interfering with LC3 or Beclin1, which is consistent with the effect of autophagy inhibitors on SVCV 36 .…”

Section: The Roles Of Autophagy In Aquatic Animal Virus Infectionmentioning

confidence: 54%

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Autophagy regulation of virus infection in aquatic animals

Zhang

Zhao

et al. 2023

Reviews in Aquaculture

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Autophagy is a conserved intracellular degradation process that is required to maintain host homeostasis and cope with invading pathogens. Over the past few decades, studies on mammals have greatly increased our understanding of the relationship between autophagy and virus infection. Autophagy may convey the invader to lysosomes to degrade or activate the host immune response against virus replication. However, many viruses have developed some strategies that evade the degradative nature of autophagy or hijack this pathway for their gain. It follows that autophagy during viral infection is a double‐edged sword. In contrast to mammals, the review on autophagy modulated by the aquatic animal virus is limited. Here, after a brief description of the main information about autophagy, we highlight current progress on the interplays between autophagy and virus infection in aquatic animals, including the phenomenon of autophagy upon virus infection, the effect of modulating autophagy on virus replication, and the crosstalk between autophagy and immune response during virus infection. This review will help us better understand the pathogenic mechanism of aquatic animal viruses and develop proper antiviral countermeasures aimed at modulating autophagy.

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Section: Crosstalk Between Autophagy and Immune Response During Aquat...supporting

confidence: 74%

Section: The Roles Of Autophagy In Aquatic Animal Virus Infectionmentioning

confidence: 54%

Autophagy regulation of virus infection in aquatic animals

Zhang

Zhao

et al. 2023

Reviews in Aquaculture

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“…The results have shown that different ATG members are stimulated after virus infection in various species. For instance, in olive ounder Paralichthys olivaceus, the mRNA level of ATG6 signi cantly increased after viral hemorrhagic septicemia virus (VHSV) infection [39]. In Procambarus clarkii, ATG14 expression was initially upregulated upon WSSV infection and then stabilized [26].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of ATG genes and their expression profiles under biotic and abiotic stresses in Fenneropenaeus chinensis

Guan,

Li,

Wang

et al. 2024

Preprint

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Background Autophagy is a conserved catabolic process in eukaryotes that contributes to cell survival in response to multiple stresses and is important for organism fitness. Extensive research has shown that autophagy plays a pivotal role in both viral infection and replication processes. Despite the increasing research dedicated to autophagy, investigations into shrimp autophagy are relatively scarce. Results Based on three different methods, a total of 20 members of the ATGs were identified from F. chinensis, all of which contained an autophagy domain. These genes were divided into 18 subfamilies based on their different C-terminal domains, and were found to be located on 16 chromosomes. Quantitative real-time PCR (qRT-PCR) results showed that ATG genes were extensively distributed in all the tested tissues, with the highest expression levels were detected in muscle and eyestalk. To clarify the comprehensive roles of ATG genes upon biotic and abiotic stresses, we examined their expression patterns. The expression levels of multiple ATGs showed an initial increase followed by a decrease, with the highest expression levels observed at 6 h and/or 24 h after WSSV injection. The expression levels of three genes (ATG1, ATG3, and ATG4B) gradually increased until 60 h after injection. Under low-salt conditions, 12 ATG genes were significantly induced, and their transcription abundance peaked at 96 h after treatment. Conclusions These results suggested that ATG genes may have significant roles in responding to various environmental stressors. Overall, this study provides a thorough characterization and expression analysis of ATG genes in F. chinensis, laying a strong foundation for further functional studies and promising potential in innate immunity.

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“…Transportation of cytoplasmic cargo to lysosomes is dependent on autophagy since this process contributes to homeostasis of organelles and proteins [7]. Autophagy involves autophagy-related genes ( ATGs ) present in yeast and higher eukaryotes [8], but only a few ATGs have been cloned and characterized from fish, including Beclin1 in Gobiocypris rarus [9], Paralichthys olivaceus [10] and common carp ( Cyprinus carpio ) [11], ATG5 in Danio rerio [12] and ATG4 in Pelteobagrus fulvidraco [13]. A recent study identified ATGs in Macrobrachium rosenbergii transcriptome data and examined the presence of key ATG proteins in tissues using western blotting [7].…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Four Autophagy-Related Genes That Are Expressed in Response to Hypoxia in the Brain of the Oriental River Prawn (Macrobrachium nipponense)

Sun

et al. 2019

IJMS

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Autophagy is a cytoprotective mechanism triggered in response to adverse environmental conditions. Herein, we investigated the autophagy process in the oriental river prawn (Macrobrachium nipponense) following hypoxia. Full-length cDNAs encoding autophagy-related genes (ATGs) ATG3, ATG4B, ATG5, and ATG9A were cloned, and transcription following hypoxia was explored in different tissues and developmental stages. The ATG3, ATG4B, ATG5, and ATG9A cDNAs include open reading frames encoding proteins of 319, 264, 268, and 828 amino acids, respectively. The four M. nipponense proteins clustered separately from vertebrate homologs in phylogenetic analysis. All four mRNAs were expressed in various tissues, with highest levels in brain and hepatopancreas. Hypoxia up-regulated all four mRNAs in a time-dependent manner. Thus, these genes may contribute to autophagy-based responses against hypoxia in M. nipponense. Biochemical analysis revealed that hypoxia stimulated anaerobic metabolism in the brain tissue. Furthermore, in situ hybridization experiments revealed that ATG4B was mainly expressed in the secretory and astrocyte cells of the brain. Silencing of ATG4B down-regulated ATG8 and decreased cell viability in juvenile prawn brains following hypoxia. Thus, autophagy is an adaptive response protecting against hypoxia in M. nipponense and possibly other crustaceans. Recombinant MnATG4B could interact with recombinant MnATG8, but the GST protein could not bind to MnATG8. These findings provide us with a better understanding of the fundamental mechanisms of autophagy in prawns.

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Molecular characterization of the autophagy-related gene Beclin-1 from the olive flounder (Paralichthys olivaceus)

Cited by 23 publications

References 35 publications

Autophagy regulation of virus infection in aquatic animals

Autophagy regulation of virus infection in aquatic animals

Genome-wide identification of ATG genes and their expression profiles under biotic and abiotic stresses in Fenneropenaeus chinensis

Identification and Characterization of Four Autophagy-Related Genes That Are Expressed in Response to Hypoxia in the Brain of the Oriental River Prawn (Macrobrachium nipponense)

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