Molecular characterization of the autochthonous microbiota in the gastrointestinal tract of adult yellow grouper (Epinephelus awoara) cultured in cages

Zhou, Zhigang; Liu, Yuchun; Shi, Pengjun; He, Shunping; Yao, Bin; Ringø, Einar

doi:10.1016/j.aquaculture.2008.10.002

Cited by 67 publications

(68 citation statements)

References 28 publications

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“…This bacterial community is markedly different from that of intestine, where Proteobacteria is predominant, followed by Fusobacteria and Firmicutes (Wu et al 2010). The finding that the difference in bacterial composition in the stomach and intestine is consistent with Zhou et al (2009), who concluded from the adult yellow grouper (Epinephelus awoara).…”

Section: Discussionsupporting

confidence: 82%

“…Navarrete et al (2009) reported the average bacterial density to be 1 9 10 7 and 5 9 10 7 bacteria g -1 in the stomach and intestine, respectively, in Salmo salar using epifluorescence microscopy. Zhou et al (2009) disclosed the difference in bacterial composition in the stomach and intestine of adult yellow grouper (Epinephelus awoara), where Empedobacter sp. PH7-1 and Acinetobacter sp.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)

Tian

Wang

et al. 2012

World J Microbiol Biotechnol

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In this study, the traditional culture-based technique and the 16S rDNA sequencing method were used to investigate the characterization of bacterial community in the stomach contents and mucus of yellow catfish (Pelteobagrus fulvidraco). The culture-based technique disclosed that the average bacterial numbers in the gastric contents and mucus were 5.79 9 10 7 cfu/g (cfu: colony forming unit) and 1.89 9 10 5 cfu/g, respectively. Several different bacteria were obtained from gastric contents, including species from genera Bradyrhizobium, Phyllobacterium, Plesiomonas, Hafnia, Edwardsiella, Pseudomonas, and Bacillus. However, only two species were isolated from the gastric mucus, including species from genera Plesiomonas and Aeromonas. Forty-five phylotypes were observed from the 65 positive clones from the stomach contents (library SC); nineteen phylotypes were detected from the 45 clones from the stomach mucus (library SM). Further analyses revealed that the fish stomach harbored characteristic microbiota, where Firmicutes was dominant, followed by Proteobacteria and Bacteroidetes and Fusobacteria. This characterization of bacterial community is markedly different from that of the fish intestine, where Proteobacteria is predominant, followed by Fusobacteria and Firmicutes. Chloroflexi (1.5%) was only found in the library SC, while Actinobacteria (4.4%) was only found in the library SM, suggesting that microbiota of GI contents was quite different from that of GI mucus. In addition, several species of bacteria found in the stomach may be potentially opportunistic pathogens, indicating that fish digestive tract is a reservoir for many nosocomial pathogens.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)

Tian

Wang

et al. 2012

World J Microbiol Biotechnol

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“…Our results suggested that the dominant bacterial species from both library C and library M belonged to Proteobacteria. This is consistent with Kim et al (2007) and Zhou et al (2009), who demonstrated that the most common bacteria from fish intestine were affiliated with Proteobacteria. However, compared with library M, library C showed much higher microflora diversities as the bacterial groups OP10 and Actinobacteria occurred.…”

Section: Discussionsupporting

confidence: 77%

Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco)

et al. 2010

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“…A vast number of uncharacterized microorganisms restrict analysis of GI microbiota using traditional culture approaches. Alternative approaches, such as denaturing gradient gel electrophoresis (DGGE) based on 16S rRNA genes, have been widely used to study the microbial community composition in fish intestine (Tannock, 2001;Wielen et al, 2002;De La Cochetière et al, 2008;Zhou et al, 2009). The genetic relationships of the four fish species were explored by sequencing the mitochondrial cytochrome c oxidase I (COI) gene, an efficient DNA "barcoding" technique for identifying freshwater fish species (Hubert et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Host species as a strong determinant of the intestinal microbiota of fish larvae

et al. 2012

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We investigated the influence of host species on intestinal microbiota by comparing the gut bacterial community structure of four cohabitating freshwater fish larvae, silver carp, grass carp, bighead carp, and blunt snout bream, using denaturing gradient gel electrophoresis (DGGE) of the amplified 16S and 18S rRNA genes. Similarity clustering indicated that the intestinal microbiota derived from these four fish species could be divided into four groups based on 16S rRNA gene similarity, whereas the eukaryotic 18S rRNA genes showed no distinct groups. The water sample from the shared environment contained microbiota of an independent group as indicated by both 16S and 18S rRNA genes segments. The bacterial community structures were visualized using rank-abundance plots fitted with linear regression models. Results showed that the intestinal bacterial evenness was significantly different between species (P<0.05) and between species and the water sample (P<0.01). Thirty-five relatively dominant bands in DGGE patterns were sequenced and grouped into five major taxa: Proteobacteria (26), Actinobacteria (5), Bacteroidetes (1), Firmicutes (2), and Cyanobacterial (1). Six eukaryotes were detected by sequencing 18S rRNA genes segments. The present study suggests that the intestines of the four fish larvae, although reared in the same environment, contained distinct bacterial populations, while intestinal eukaryotic microorganisms were almost identical.

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Molecular characterization of the autochthonous microbiota in the gastrointestinal tract of adult yellow grouper (Epinephelus awoara) cultured in cages

Cited by 67 publications

References 28 publications

Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)

Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)

Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco)

Host species as a strong determinant of the intestinal microbiota of fish larvae

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