Molecular characterization of swine leucocyte antigen class I genes in outbred pig populations

Ho, Chak-Sum; Lunney, Joan K.; Franzo-Romain, M. H.; Martens, Gregory W.; Lee, Y. -J.; Lee, J. -H.; Wysocki, Michał; Rowland, R.; Smith, Douglas M.

doi:10.1111/j.1365-2052.2009.01860.x

Cited by 65 publications

(101 citation statements)

References 34 publications

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“…Gilts 1–3 and the nine piglets used in this study were genotyped for three swine leukocyte antigen (SLA) class I genes (SLA-1, -2, -3) and three class II genes (DRB1, DQB1, DQA) using the low resolution PCR-SSP (polymerase chain reaction with sequence-specific primers) typing panels as previously described [49,50]. Low resolution SLA class I and class II haplotypes were deduced based on published (15713212, 16305679, 18760302, 19317739) and unpublished (http://www.jimmunol.org/cgi/content/meetingabstract/186/1_MeetingAbstracts/170.1) haplotypes identified in outbred commercial pigs.…”

Section: Methodsmentioning

confidence: 99%

Recognition of Highly Diverse Type-1 and -2 Porcine Reproductive and Respiratory Syndrome Viruses (PRRSVs) by T-Lymphocytes Induced in Pigs after Experimental Infection with a Type-2 PRRSV Strain

Chung

Cha

Grimm³

et al. 2016

PLoS ONE

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Background/AimLive attenuated vaccines confer partial protection in pigs before the appearance of neutralizing antibodies, suggesting the contribution of cell-mediated immunity (CMI). However, PRRSV-specific T-lymphocyte responses and protective mechanisms need to be further defined. To this end, the hypothesis was tested that PRRSV-specific T-lymphocytes induced by exposure to type-2 PRRSV can recognize diverse isolates.MethodsAn IFN-gamma ELISpot assay was used to enumerate PRRSV-specific T-lymphocytes from PRRSVSD23983-infected gilts and piglets born after in utero infection against 12 serologically and genetically distinct type-1 and -2 PRRSV isolates. The IFN-gamma ELISpot assay using synthetic peptides spanning all open reading frames of PRRSVSD23983 was utilized to localize epitopes recognized by T-lymphocytes. Virus neutralization tests were carried out using the challenge strain (type-2 PRRSVSD23983) and another strain (type-2 PRRSVVR2332) with high genetic similarity to evaluate cross-reactivity of neutralizing antibodies in gilts after PRRSVSD23983 infection.ResultsAt 72 days post infection, T-lymphocytes from one of three PRRSVSD23983-infected gilts recognized all 12 diverse PRRSV isolates, while T-lymphocytes from the other two gilts recognized all but one isolate. Furthermore, five of nine 14-day-old piglets infected in utero with PRRSVSD23983 had broadly reactive T-lymphocytes, including one piglet that recognized all 12 isolates. Overlapping peptides encompassing all open reading frames of PRRSVSD23983 were used to identify ≥28 peptides with T-lymphocyte epitopes from 10 viral proteins. This included one peptide from the M protein that was recognized by T-lymphocytes from all three gilts representing two completely mismatched MHC haplotypes. In contrast to the broadly reactive T-lymphocytes, neutralizing antibody responses were specific to the infecting PRRSVSD23983 isolate.ConclusionThese results demonstrated that T-lymphocytes recognizing antigenically and genetically diverse isolates were induced by infection with a type 2 PRRSV strain (SD23983). If these reponses have cytotoxic or other protective functions, they may help overcome the suboptimal heterologous protection conferred by conventional vaccines.

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Section: Methodsmentioning

confidence: 99%

Recognition of Highly Diverse Type-1 and -2 Porcine Reproductive and Respiratory Syndrome Viruses (PRRSVs) by T-Lymphocytes Induced in Pigs after Experimental Infection with a Type-2 PRRSV Strain

Chung

Cha

Grimm³

et al. 2016

PLoS ONE

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“…Especially, SLA haplotype Lr-56.30 was one of the most prevalent haplotype in KNPs from Jeju province (Cho et al, 2010). In this study, all the pigs investigated had Lr-56.30, indicating that two different KNP populations (Jeju and Cheongyang) were originated from (Ho et al, 2009b;Ho et al, 2010).…”

Section: Resultsmentioning

confidence: 52%

“…On the other hand, SLA class III genes having wide range of functions and some of them are related to the complement cascade (Naziruddin et al, 1998). In 2002, SLA Nomenclature Committee of the International Society for Animal Genetics (ISAG) has been established for the systematic nomenclature of the class I and class II SLA alleles (Smith et al, 2005a;Smith et al, 2005b;Ho et al, 2009a;Ho et al, 2009b;Ho et al, 2010a). The recent updates of SLA alleles can also be found in Immunopolymorphism Database-Major Histocompatibility Complex (IPD-MHC) website (http://www.ebi.ac.…”

Section: Introductionmentioning

confidence: 99%

SLA Homozygous Korean Native Pigs and Their Inbreeding Status Deduced from the Microsatellite Marker Analysis

Jung¹,

Lim²,

Lim³

et al. 2010

Journal of Animal Science and Technology

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The porcine MHC (Major Histocompatibility Complex), encoding the SLA (Swine Leukocyte Antigen) genes, is one of the most significant regions associated with immune rejection in relation to transplantation. In this study, three SLA class I (SLA-1, SLA-3, SLA-2) loci and three SLA class II (DRB1, DQB1, DQA) loci were investigated in the previously unidentified Korean native pig (KNP) population that was closely inbred in the Livestock Technology Research Station in Cheongyang, Korea. Total thirteen KNPs from four generations were genotyped for the SLA alleles and haplotypes were investigated using PCR-SSP (SequenceSpecific Primer) method. The results showed that all of these KNPs had Lr-56.30/56.30 homozygous haplotype, indicating high level of inbreeding in the SLA genes. The inbreeding status of these animals was also investigated using microsatellite (MS) markers. From the 50 MS markers investigated, 17 MS markers were fixed in all generations and the fixed alleles are increased as 26 loci for the fourth generation. Two MS markers, S0069 and SW173, were heterozygous for all the animals tested. Observed and expected heterozygosities were calculated and the average inbreeding coefficients for each generation were also calculated. In the fourth generation, the average inbreeding coefficients was 0.732 and this may increase with further inbreeding process. Analysis of the SLA haplotypes and MS alleles can give important information for breeding the pigs for xenotransplantation studies.

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“…Experimental animals received chemically (C 3 H 4 O 2 ) inactivated swine influenza A virus of different strains given in equal volumes of Freund’s Incomplete adjuvant with 4 repeated immunizations at three-week intervals (Table 1). Initially, blood samples were collected from all pigs followed by SLA allele typing using PCR-SSP [14–16]. Candidate SwIV epitopes were selected using in silico predictions for binding by the online available NetMHCpan algorithm [17–19], and combined with previously mapped preferences expressed by SLA-1*0401 [13].…”

Section: Methodsmentioning

confidence: 99%

Identification of swine influenza virus epitopes and analysis of multiple specificities expressed by cytotoxic T cell subsets

Pedersen

Breum

Riber

et al. 2014

Virol J

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BackgroundMajor histocompatibility complex (MHC) class I peptide binding and presentation are essential for antigen-specific activation of cytotoxic T lymphocytes (CTLs) and swine MHC class I molecules, also termed swine leukocyte antigens (SLA), thus play a crucial role in the process that leads to elimination of viruses such as swine influenza virus (SwIV). This study describes the identification of SLA-presented peptide epitopes that are targets for a swine CTL response, and further analyses multiple specificities expressed by SwIV activated CTL subsets.FindingsFour SwIV derived peptides were identified as T cell epitopes using fluorescent influenza:SLA tetramers. In addition, multiple CTL specificities were analyzed using peptide sequence substitutions in two of the four epitope candidates analyzed. Interestingly both conserved and substituted peptides were found to stain the CD4-CD8+ T cell subsets indicating multiple specificities.ConclusionsThis study describes a timely and cost-effective approach for viral epitope identification in livestock animals. Analysis of T cell subsets showed multiple specificities suggesting SLA-bound epitope recognition of different conformations.

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Molecular characterization of swine leucocyte antigen class I genes in outbred pig populations

Cited by 65 publications

References 34 publications

Recognition of Highly Diverse Type-1 and -2 Porcine Reproductive and Respiratory Syndrome Viruses (PRRSVs) by T-Lymphocytes Induced in Pigs after Experimental Infection with a Type-2 PRRSV Strain

Recognition of Highly Diverse Type-1 and -2 Porcine Reproductive and Respiratory Syndrome Viruses (PRRSVs) by T-Lymphocytes Induced in Pigs after Experimental Infection with a Type-2 PRRSV Strain

SLA Homozygous Korean Native Pigs and Their Inbreeding Status Deduced from the Microsatellite Marker Analysis

Identification of swine influenza virus epitopes and analysis of multiple specificities expressed by cytotoxic T cell subsets

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