Molecular characterization of swine hepatitis E virus from Southeastern Brazil

Paiva, Helder Henrique; Tzaneva, Valentina; Haddad, Rodrigo; Yokosawa, Jonny

doi:10.1590/s1517-83822007000400020

Cited by 20 publications

(31 citation statements)

References 50 publications

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“…Even though the sequences were not compared to the strain described by Paiva et al (2007), these isolates showed to be closely related to each other but only distantly related to Argentine HEV isolates, suggesting that they probably had different origins.…”

Section: Molecular Epidemiologymentioning

confidence: 91%

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Molecular Epidemiology of Hepatitis E Virus (Hev) in South America: Current Status

Mirazo¹,

Ramos²,

Arbiza³

2012

VR&R

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Hepatitis E virus (HEV) is a common causative agent of acute hepatitis in many developing countries where standards of sanitation and hygiene are poor. HEV is transmitted primarily by the fecal-oral route. However, zoonotic trasmission from animal reservoirs to human by consumption of raw or undercooked meat has also been suggested. In endemic areas, HEV infection occurs as large waterborne epidemics and/or small outbreaks. Sporadic cases of HEV infection have also been reported in developed countries, where its occurrence is not always associated with travel to highly endemic areas. HEV mammalian isolates are classifi ed into four genotypes 1-4, which appear to have a specifi c host range and geographical distribution. The epidemiology of HEV in South America seems to be complex and seroprevalence in human and domestic pigs diff ers both among populations within the same countries and between countries. Additionally, HEV strains have been detected and molecularly characterized and genotype 3 is the most frequently genotype found in the region. Moreover, except for two strains isolated from autochthonous cases of acute hepatitis that occurred in Venezuela and belonged to genotype 1, all sequences detected in South America were classifi ed as within genotype 3. Our understanding of HEV epidemiology has undergone major changes in recent years and despite the fact that several attempts to shed light over the epidemiology of this infection have been carried out in South America, data about the molecular characterization of HEV isolates is still lacking and further investigation is needed. This review summarizes the current status of HEV molecular epidemiology from a regional point of view.

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Section: Molecular Epidemiologymentioning

confidence: 91%

“…In fact, the first HEV strain described, classified as genotype 3 by full-length genome molecular analysis, was isolated from a pig in 2007, when no cases of HEV infection in humans had been reported in this country (Paiva et al 2007).…”

Section: Molecular Epidemiologymentioning

confidence: 99%

Molecular Epidemiology of Hepatitis E Virus (Hev) in South America: Current Status

Mirazo¹,

Ramos²,

Arbiza³

2012

VR&R

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“…To detect IgG antibodies against HEV in pigs, the commercial kit of indirect ELISA HEV-IGAB (Dia.Pro-Diagnostic Bioprobes, Italy) for dual detection of IgG and IgM antibodies in human serum was used, replacing the antihuman conjugate with a conjugated goat antibody anti-IgG for swine (Thermo Fisher Scientific Inc, USA) according to the methodology used by other researchers (17,(21)(22)(23) at the dilution recommended by the manufacturer (1:5000). As a negative swine control (NPC), a serum of newborn piglets (in triplicate) that did not ingest colostrum was used, to avoid the presence of maternal antibodies; and as a positive control (CPP) a sample of the study that showed high OD values in the ELISA was employed.…”

Section: Materiales Y Métodosmentioning

confidence: 99%

“…To ensure the viability of the modified conjugated immunoassays with the anti-swine conjugates, initially a positive swine serum sample for IgG anti-HEV characterized and evaluated in a previous study, kindly donated by Dr. Munné (National Reference Laboratory for viral hepatitis INEI ANLIS "Carlos Malbrán", Argentina), (17). This serum was positive with the selected cutoff point in this project, but preliminary tests indicated that the optical density reading at 450 nm (OD 450) for the Argentine positive serum were two times lower than DO 450 of some Colombian samples (data not shown); for this reason, and because the volume of the donated positive serum was insufficient to use as a positive control in all assays, the CPP and CNP described above were used.…”

Section: Viability Of the Indirect Modified Elisa Test To Detect Igg mentioning

confidence: 99%

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Serological evidence of Hepatitis E Virus infection in Antioquia, Colombia slaughtered pigs

Gutiérrez

et al. 2015

Rev MVZ Córdoba

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Objective. To detect the presence of specific antibodies against Hepatitis E virus (HEV) in pigs slaughtered in Antioquia, the department where the greatest amount of pork is produced and consumed in Colombia. Materials and methods. Between September 2011 and May 2012, blood samples from pigs were obtained in five slaughterhouses of Antioquia, four of them located in the Aburrá Valley subregion and other located in northern subregión. Serum were evaluated with a commercial ELISA kit for diagnosing HEV in humans but adapted to detect IgG and IgM antibodies in pigs. Results. A 100.0% seropositivity for IgG antibodies was found in 1000 samples evaluated, and 82.06% for IgM antibodies were found in 740 samples. Conclusions. These results indicate that pigs in slaughter age in Antioquia, and possibly in Colombia, have been exposed to HEV at some point in their production process and a high percentage of them can arrive to slaughterhouses with recent infection.

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First report and molecular characterization of hepatitis E virus infection in renal transplant recipients in Brazil

Passos

Heringer

Medina-Pestana

et al. 2013

Journal of Medical Virology

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Hepatitis E virus (HEV) causes acute and chronic hepatitis in organ transplant recipients. Serological evidence for HEV infection has been discovered in various population groups in Brazil, and a single acute case has been confirmed. To date, however, no cases of HEV infection in immunocompromised patients have been reported in Brazil. This study aimed to identify and characterize hepatitis E cases in renal transplant recipients in Brazil. A retrospective study was performed on 96 serum samples from renal transplant recipients with unexplained liver enzymes elevation. Three confirmed cases of HEV infection were identified that lacked seroconversion to HEV IgG antibodies. The prevalence of HEV in these patients was 3.1%. Using a sequence analysis of a 304-nucleotide fragment within ORF2, the strains were classified as genotype 3 with a low percent identity to previously characterized strains. This is the first report of hepatitis E infection in renal transplant recipients in Brazil, and the data indicate that a novel genotype 3 subvariant may be present and that further investigation is necessary to characterize the circulating HEV strains. In this setting, HEV infection should be considered as a potential cause of abnormal liver tests of unknown origin.

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Molecular characterization of swine hepatitis E virus from Southeastern Brazil

Cited by 20 publications

References 50 publications

Molecular Epidemiology of Hepatitis E Virus (Hev) in South America: Current Status

Molecular Epidemiology of Hepatitis E Virus (Hev) in South America: Current Status

Serological evidence of Hepatitis E Virus infection in Antioquia, Colombia slaughtered pigs

First report and molecular characterization of hepatitis E virus infection in renal transplant recipients in Brazil

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