Molecular characterization of sweet potato leaf curl virus (SPLCV) isolates from Korea: phylogenetic relationship and recombination analysis

J, Park; S, Kim; Choi, Eun‐Jin; Hr, Kwak; Mk, Kim; Ky, Lee; Choi, HS; S, Lee

doi:10.4149/av_2011_04_327

Cited by 8 publications

(4 citation statements)

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“…Detection of SPLCV in vegetative tissues of sweet potatoes with symptoms A PCR detection system was set up with DNA isolated from the leaves of healthy and SPLCV-infected sweet potato plants as reported previously (Park et al, 2010;Choi et al, 2012). Ten plants each from eight cultivars showing typical leaf curl disease symptoms were tested by PCR and 8-10 plants per cultivar showed SPLCV-specific amplification ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…The SPLCV monopartite genome has two open reading frames (ORF) (V1 and V2) in the virion sense and four ORFs (C1, C2, C3 and C4) in the complementary sense, separated by an intergenic region (IR) containing a conserved stem-loop motif, in a 2Á8-kb single-stranded circular DNA (Lotrakul & Valverde, 1999). Complete genome sequences of SPLCV isolates in Korea have been characterized, and their phylogenetic relationships with other sweepovirus species have been determined (Park et al, 2010;Choi et al, 2012). SPLCV and related sweepoviruses have been found in many countries, including Brazil, China, India, Italy, Kenya, Korea, Peru, Spain and Uganda (Banks et al, 1999;Briddon et al, 2006;Kwak et al, 2006;Luan et al, 2006;Miano et al, 2006;Prasanth & Hegde, 2008;Lozano et al, 2009;Paprotka et al, 2010;Albuquerque et al, 2011;Wasswa et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Seed transmission of Sweet potato leaf curl virus in sweet potato (Ipomoea batatas)

et al. 2015

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Sweet potato leaf curl virus (SPLCV) infects sweet potato and is a member of the family Geminiviridae (genus Begomovirus). SPLCV transmission occurs from plant to plant mostly via vegetative propagation as well as by the insect vector Bemisia tabaci. When sweet potato seeds were planted and cultivated in a whitefly‐free greenhouse, some sweet potato plants started to show SPLCV‐specific symptoms. SPLCV was detected by PCR from all leaves and floral tissues that showed leaf curl disease symptoms. More than 70% of the seeds harvested from SPLCV‐infected sweet potato plants tested positive for SPLCV. SPLCV was also identified from dissected endosperm and embryos. The transmission level of SPLCV from seeds to seedlings was up to 15%. Southern blot hybridization showed SPLCV‐specific single‐ and double‐stranded DNAs in seedlings germinated from SPLCV‐infected seeds. Taken altogether, the results show that SPLCV in plants of the tested sweet potato cultivars can be transmitted via seeds and SPLCV DNA can replicate in developing seedlings. This is the first seed transmission report of SPLCV in sweet potato plants and also, to the authors' knowledge, the first report of seed transmission for any geminivirus.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seed transmission of Sweet potato leaf curl virus in sweet potato (Ipomoea batatas)

et al. 2015

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“…Sweepoviruses have been reported in many countries including Peru, Italy, Spain, China, Korea, Kenya, Uganda, India, and Brazil [ 1 , 4 , 21 – 29 ]. Complete genome sequences of SPLCV isolates in Korea have been characterized, and their phylogenetic relationships with other Sweepovirus species have been determined [ 30 , 31 ]. Moreover, complete genome information of Sweepovirus is the most plentiful virus species among all sweet potato-infecting viruses [ 28 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Phylogeographic analysis of the full genome of Sweepovirus to trace virus dispersal and introduction to Korea

et al. 2018

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Sweet potato is a vegetatively propagated crop that is produced for both growth in Korean fields and for export out of the country. The viruses that are present in introduced sweet potatoes can spread both domestically and to foreign countries. Determining the time and path of virus movement could help curtail its spread and prevent future dispersal of related viruses. Determining the consequences of past virus and sweet potato dispersal could provide insight into the ecological and economic risks associated with other sweet potato-infecting viral invasions. We therefore applied Bayesian phylogeographic inferences and recombination analyses of the available Sweepovirus sequences (including 25 Korean Sweepovirus genomes) and reconstructed a plausible history of Sweepovirus diversification and movement across the globe. The Mediterranean basin and Central America were found to be the launchpad of global Sweepovirus dispersal. Currently, China and Brazil are acting as convergence regions for Sweepoviruses. Recently reported Korean Sweepovirus isolates were introduced from China in a recent phase and the regions around China and Brazil continue to act as centers of Sweepovirus diversity and sites of ongoing Sweepovirus evolution. The evidence indicates that the region is an epidemiological hotspot, which suggests that novel Sweepovirus variants might be found.

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“…A recent study reported that SPLCV can be transmitted via seed ( Kim et al, 2015 ). Korean isolates of SPLCV were characterized and their full genome sequences were analyzed ( Choi et al, 2012 ; Park et al, 2011 ). Recently, Sweet potato golden vein associated virus was characterized and reported ( Kil et al, 2014 ).…”

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confidence: 99%

Virus Incidence of Sweet Potato in Korea from 2011 to 2014

Kim

Yang

Kwak

et al. 2017

The Plant Pathology Journal

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A nationwide survey was performed to investigate the current incidence of viral diseases in Korean sweet potatoes for germplasm and growing fields from 2011 to 2014. A total of 83.8% of the germplasm in Korea was infected with viruses in 2011. Commercial cultivars that were used to supply growing fields were infected at a rate of 62.1% in 2012. Among surveyed viruses, the incidence of five Potyvirus species that infect sweet potato decreased between 2012 and 2013, and then increased again in 2014. Representatively, the incidence of Sweet potato feathery mottle virus (SPFMV) was 87.0% in 2012, 20.7% in 2013 and then increased to 35.3% in 2014. Unlike RNA viruses, DNA viruses were shown to decrease continuously. The incidence of Sweet potato leaf curl virus (SPLCV) was 5.5% in 2003, 59.5% in 2011, and 47.4% in 2012. It then decreased continuously year by year to 33.2% in 2013, and then 25.6% in 2014. While the infection rate of each virus species showed a tendency to decline, the virus infection status was more variable in 2013 and 2014. Nevertheless, the high rate of single infections and mixed infection combinations were more variable than the survey results from 2012. As shown in the results from 2013, the most prevalent virus infection was a single infection at 27.6%, with the highest rate of infection belonging to sweet potato symptomless virus-1 (SPSMV-1) (12.9%). Compared to 2013, infection combinations were more varied in 2014, with a total of 122 kinds of mixed infection.

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Molecular characterization of sweet potato leaf curl virus (SPLCV) isolates from Korea: phylogenetic relationship and recombination analysis

Cited by 8 publications

References 28 publications

Seed transmission of Sweet potato leaf curl virus in sweet potato (Ipomoea batatas)

Seed transmission of Sweet potato leaf curl virus in sweet potato (Ipomoea batatas)

Phylogeographic analysis of the full genome of Sweepovirus to trace virus dispersal and introduction to Korea

Virus Incidence of Sweet Potato in Korea from 2011 to 2014

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