Molecular Characterization of Subfamily Schizothoracinae (Teleostei: Cyprinidae) using Complete Sequence of Mitochondrial 16S rRNA Gene

Akhtar, Tasleem; Ali, Ghazanfar; Shafi, Nuzhat; Rauf, Abdul

doi:10.17582/journal.pjz/2020.52.1.273.282

Cited by 2 publications

(1 citation statement)

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“…In the cyprinids, the phylogenetic approaches are based on the whole mitochondrial genome or the functional genes (13 PCGs) that are helpful for a better understanding of speciation and divergence [ 12 ]. Schizothoracinae (Cyprinidae) fishes represent the largest and most diverse taxon possessing more than 100 species and subspecies ( http://www.fishbase.org ) with a worldwide distribution [ 13 – 15 ]. Schizothoracinae are economically and commercially essential species living in fast-flowing and snowy rivers and streams including the Neelum and Jhelum Rivers in Azad Jammu and Kashmir [ 13 , 16 ].…”

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confidence: 99%

Sequencing and Characterization of Mitochondrial Protein-Coding Genes for Schizothorax niger (Cypriniformes: Cyprinidae) with Phylogenetic Consideration

Akhtar

Ali

Shafi

et al. 2020

BioMed Research International

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The present study was conducted to get more information about the genome and locate the taxonomic position of Schizothorax niger in Schizothoracinae through mitochondrial 13 protein-coding genes (PCGs). These PCGs for S. niger were found to be 11409 bps in length ranging from 165 (ATPase 8) to 1824 bps (NADH dehydrogenase subunit 5) and encode 3801 amino acids. In these PCGs, 4 genes overlap on the similar strands, while one shown on the opposite one: ATPase 6+8 and NADH dehydrogenase subunit 4+4L overlap by 7 nucleotides. Similarly, ND5-ND6 overlap by 4 nucleotides, while ATP6 and COIII overlap by 1 nucleotide. Similarly, four commonly used amino acids in S. niger were Leu (15.6 %), Ile (10.12 %), Thr (8.12 %), and Ala (8.7 %). The results presented that COII, COIII, NDI, ND4L, and Cytb had substantial amino acid conservation as compared to the COI gene. Through phylogenetic analysis, it was observed that S. niger is closely linked with S. progastus, S. labiatus, S. plagiostomus, and S. nepalensis with high bootstrap values. The present study provided more genomic data to know the diversity of the mitochondrial genome and its molecular evolution in Schizothoracinae.

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