Molecular characterization of stress-inducible GmNAC genes in soybean

Tran, Lam‐Son Phan; Quach, Truyen; Guttikonda, Satish K.; Aldrich, Donavan L.; Kumar, Rajesh; Neelakandan, Anjanasree K.; Valliyodan, Babu; Nguyen, Henry T.

doi:10.1007/s00438-009-0436-8

Cited by 141 publications

(144 citation statements)

References 63 publications

(98 reference statements)

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“…We therefore examined the expression of ABA responsive genes after quinabactin applications to soybean, barley, and maize, diverse agricultural species that span the monocot-dicot split. In soybean, the ABA marker genes GmNac4 and GmZIP1 (38,39) were induced by quinabactin to similar levels as those achieved with ABA treatment ( Fig. 2D; Fig.…”

Section: Resultssupporting

confidence: 61%

Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance

Okamoto

Peterson

Defries

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

261

268

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Abscisic acid (ABA) is an essential molecule in plant abiotic stress responses. It binds to soluble pyrabactin resistance1/PYR1-like/ regulatory component of ABA receptor receptors and stabilizes them in a conformation that inhibits clade A type II C protein phosphatases; this leads to downstream SnRK2 kinase activation and numerous cellular outputs. We previously described the synthetic naphthalene sulfonamide ABA agonist pyrabactin, which activates seed ABA responses but fails to trigger substantial responses in vegetative tissues in Arabidopsis thaliana. Here we describe quinabactin, a sulfonamide ABA agonist that preferentially activates dimeric ABA receptors and possesses ABA-like potency in vivo. In Arabidopsis, the transcriptional responses induced by quinabactin are highly correlated with those induced by ABA treatments. Quinabactin treatments elicit guard cell closure, suppress water loss, and promote drought tolerance in adult Arabidopsis and soybean plants. The effects of quinabactin are sufficiently similar to those of ABA that it is able to rescue multiple phenotypes observed in the ABA-deficient mutant aba2. Genetic analyses show that quinabactin's effects in vegetative tissues are primarily mediated by dimeric ABA receptors. A PYL2-quinabactin-HAB1 X-ray crystal structure solved at 1.98-Å resolution shows that quinabactin forms a hydrogen bond with the receptor/PP2C "lock" hydrogen bond network, a structural feature absent in pyrabactin-receptor/PP2C complexes. Our results demonstrate that ABA receptors can be chemically controlled to enable plant protection against water stress and define the dimeric receptors as key targets for chemical modulation of vegetative ABA responses.

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Section: Resultssupporting

confidence: 61%

Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance

Okamoto

Peterson

Defries

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

261

268

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“…cDNA synthesis and qRT-PCR were performed according to previously described methods (Tran et al, 2009). The primer pairs that were used in qRT-PCR reactions are listed in Supplemental Table 1 online.…”

Section: Transcriptional Analysesmentioning

confidence: 99%

Analysis of Cytokinin Mutants and Regulation of Cytokinin Metabolic Genes Reveals Important Regulatory Roles of Cytokinins in Drought, Salt and Abscisic Acid Responses, and Abscisic Acid Biosynthesis

et al. 2011

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Cytokinins (CKs) regulate plant growth and development via a complex network of CK signaling. Here, we perform functional analyses with CK-deficient plants to provide direct evidence that CKs negatively regulate salt and drought stress signaling. All CK-deficient plants with reduced levels of various CKs exhibited a strong stress-tolerant phenotype that was associated with increased cell membrane integrity and abscisic acid (ABA) hypersensitivity rather than stomatal density and ABA-mediated stomatal closure. Expression of the Arabidopsis thaliana ISOPENTENYL-TRANSFERASE genes involved in the biosynthesis of bioactive CKs and the majority of the Arabidopsis CYTOKININ OXIDASES/DEHYDROGENASES genes was repressed by stress and ABA treatments, leading to a decrease in biologically active CK contents. These results demonstrate a novel mechanism for survival under abiotic stress conditions via the homeostatic regulation of steady state CK levels. Additionally, under normal conditions, although CK deficiency increased the sensitivity of plants to exogenous ABA, it caused a downregulation of key ABA biosynthetic genes, leading to a significant reduction in endogenous ABA levels in CK-deficient plants relative to the wild type. Taken together, this study provides direct evidence that mutual regulation mechanisms exist between the CK and ABA metabolism and signals underlying different processes regulating plant adaptation to stressors as well as plant growth and development.

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“…Genome-wide transcriptomic analysis indicates that 20% to 25% of NAC genes in the whole genome of Arabidopsis and rice are responsive to drought stress (Tran et al, 2010). There are also a number of studies on abiotic stress-related NAC transcription factors in other plant species, such as that on GmNACs from soybean (Glycine max; Tran et al, 2009), TaNAC69 from wheat (Triticum aestivum; Xue et al, 2011), and AhNAC2 from groundnut (Arachis hypogaea; Liu et al, 2011). There has been no study, so far, of the possible role of NAC family genes in the response to WDS during petal expansion of flowers.…”

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confidence: 99%

RhNAC2 and RhEXPA4 Are Involved in the Regulation of Dehydration Tolerance during the Expansion of Rose Petals

et al. 2012

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Dehydration inhibits petal expansion resulting in abnormal flower opening and results in quality loss during the marketing of cut flowers. We constructed a suppression subtractive hybridization library from rose (Rosa hybrida) flowers containing 3,513 unique expressed sequence tags and analyzed their expression profiles during cycles of dehydration. We found that 54 genes were up-regulated by the first dehydration, restored or even down-regulated by rehydration, and once again up-regulated by the second dehydration. Among them, we identified a putative NAC family transcription factor (RhNAC2). With transactivation activity of its carboxyl-terminal domain in yeast (Saccharomyces cerevisiae) cell and Arabidopsis (Arabidopsis thaliana) protoplast, RhNAC2 belongs to the NAC transcription factor clade related to plant development in Arabidopsis. A putative expansin gene named RhEXPA4 was also dramatically up-regulated by dehydration. Silencing RhNAC2 or RhEXPA4 in rose petals by virusinduced gene silencing significantly decreased the recovery of intact petals and petal discs during rehydration. Overexpression of RhNAC2 or RhEXPA4 in Arabidopsis conferred strong drought tolerance in the transgenic plants. RhEXPA4 expression was repressed in RhNAC2-silenced rose petals, and the amino-terminal binding domain of RhNAC2 bound to the RhEXPA4 promoter. Twenty cell wall-related genes, including seven expansin family members, were up-regulated in Arabidopsis plants overexpressing RhNAC2. These data indicate that RhNAC2 and RhEXPA4 are involved in the regulation of dehydration tolerance during the expansion of rose petals and that RhEXPA4 expression may be regulated by RhNAC2.

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Molecular characterization of stress-inducible GmNAC genes in soybean

Cited by 141 publications

References 63 publications

Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance

Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance

Analysis of Cytokinin Mutants and Regulation of Cytokinin Metabolic Genes Reveals Important Regulatory Roles of Cytokinins in Drought, Salt and Abscisic Acid Responses, and Abscisic Acid Biosynthesis

RhNAC2 and RhEXPA4 Are Involved in the Regulation of Dehydration Tolerance during the Expansion of Rose Petals

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