Molecular characterization of Staphylococcus aureus isolates causing skin and soft tissue infections (SSTIs)

Yao, Dan; Yu, Fangyou; Qin, Zhiqiang; Chen, Chun; He, Shuhua; Chen, Zengqiang; Zhang, Xueqing; Wang, Liangxing

doi:10.1186/1471-2334-10-133

Cited by 55 publications

(47 citation statements)

References 25 publications

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“…The presence of C97 and C151 was congruent with the findings of recent publications, which reported them as the most predominant clusters associated with bovine mastitis in a number of countries worldwide (9,16,28). C20 was described for human carriage (22) and infection (33), as well as for bovine mastitis in southern Germany and Japan (10,16). C8 is typically a human genotype which gave rise to prominent MRSA (20), but it has only sporadically been reported for animals, including horses in Germany (MRSA ST254) (32) and bovine mastitis in the Netherlands (ST8, 8% of isolates) (30).…”

Section: Discussionsupporting

confidence: 78%

Link between Genotype and Antimicrobial Resistance in Bovine Mastitis-Related Staphylococcus aureus Strains, Determined by Comparing Swiss and French Isolates from the Rhône Valley

Sakwińska

Morisset

Madec³

et al. 2011

Appl Environ Microbiol

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Staphylococcus aureus is a major bovine mastitis pathogen. Although the reported antimicrobial resistance was generally low, the emergence of new genetic clusters in bovine mastitis requires examination of the link between antimicrobial resistance and genotypes. Here, amplified fragment length polymorphism (AFLP) profiles and standard antimicrobial resistance profiles were determined in order to characterize a total of 343 S. aureus cow mastitis isolates from two geographically close regions of Switzerland and France. AFLP profiles revealed similar population compositions in the two regions, with 4 major clusters (C8, C20, C97, and C151), but the proportions of isolates in each cluster significantly diverged between the two countries (P ‫؍‬ 9.2 ؋ 10 ؊9 ). Antimicrobial resistance was overall low (<5% resistance to all therapeutically relevant molecules), with the exception of penicillin resistance, which was detected in 26% of the isolates. Penicillin resistance proportions differed between clusters, with only 1 to 2% of resistance associated with C20 and C151 and up to 70% associated with bovine C97. The prevalence of C20 and C8 was unexpectedly high and requires further investigation into the mechanism of adaptation to the bovine host. The strong association of penicillin resistance with few clusters highlights the fact that the knowledge of local epidemiology is essential for rational choices of antimicrobial treatment in the absence of susceptibility testing. Taken together, these observations argue in favor of more routine scrutiny of antimicrobial resistance and antibiotic-resistant clones in cattle and the farm environment.

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Section: Discussionsupporting

confidence: 78%

Link between Genotype and Antimicrobial Resistance in Bovine Mastitis-Related Staphylococcus aureus Strains, Determined by Comparing Swiss and French Isolates from the Rhône Valley

Sakwińska

Morisset

Madec³

et al. 2011

Appl Environ Microbiol

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“…The ST6 MRSA strain has rarely been isolated, but community-associated MRSA (CA-MRSA) clones were reported in Kuwait hospitals (32), Japan (12), and Lebanon (31). According to previous research, ST6 is not the predominant S. aureus lineage that is observed in hospitals and animals in China (6,8,9,35,36,38,40,41). Why ST6 MSSA became the dominant ST that causes SFP and whether ST6 was the unique ST in food products or among SFP isolates in China should be investigated further.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Staphylococcus aureus Strains Associated with Food Poisoning in Shenzhen, China

Yan

Wang

Tao

et al. 2012

Appl Environ Microbiol

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ABSTRACTTo characterize isolates ofStaphylococcus aureusthat were associated with staphylococcal food poisoning between 2006 and 2009 in Shenzhen, Southern China, a total of 52Staphylococcus aureusisolates from 11 outbreaks were analyzed by using multilocus sequence typing (MLST),spatyping, and pulsed-field gel electrophoresis (PFGE). PCR analysis was used to analyze the staphylococcal enterotoxin (SE) genesseatosei, and antimicrobial susceptibility testing was also performed. ST6 was the most dominant sequence type (ST), constituting 63.5% (34/52) of all of the isolates in 7 outbreaks. The next most common ST was ST943, which constituted 23.1% (12/52) of the isolates that were collected from 3 outbreaks. t701, t091, and t2360 were the most predominantspatypes, constituting 67.3% (35/52) of the isolates that were collected from 11 outbreaks. Three PFGE types, (types A, B, and C) were the most frequently observed types, constituting 84.6% (44/52) of all of the isolates. The enterotoxin gene that we detected most frequently wassea(45/52; 86.5%). Four SE gene profiles were observed, includingsea(n= 45),sec-seh(n= 3),seb(n= 2), andseg-sei(n= 2). With respect to antibiotic resistance, penicillin resistance was the most common (96.2%; 50/52), followed by resistance to tetracycline (28.8%; 15/52). Approximately 30.8% (16/52) of the isolates were resistant to at least two antibiotics, and 7.7% (4/52) of the isolates were resistant to three or more drugs. The two predominantS. aureuslineages, (i) PFGE types A and B with ST6 and (ii) PFGE type C with ST943, were identified in the outbreaks.

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“…ST88 with PVL-positive isolates had been reported in Sweden, Brazil, Italy and Asia (Afroz et al, 2008;Fang et al, 2008;Reinert et al, 2008;Stefani et al, 2009). In China, this clone was the predominant lineages of PVL-positive clones and correlated to skin and soft tissue infection (Yao et al, 2010). One pediatric clone (ST-MRSA-VI) possessing PVL-gene had been detected in our hospital.…”

Section: Mlst-ccmentioning

confidence: 86%

Molecular characteristic of Staphylococcus aureus isolates in a Chinese teaching hospital

Ma¹,

Chen²,

Zhou³

et al. 2011

Afr. J. Microbiol. Res.

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The aim of this study is to investigate the genetic backgrounds of methicillin susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA) and heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) isolates isolated from clinical specimens of the patients with verified infections in a Chinese teaching hospital. Macro E test (MET) was used to detect hVISA and multilocus sequence typing (MLST) was used to determine the STs of the selected isolates. The genotypes of SCCmec were determined by multiplex polymerase chain reaction (PCR) in MRSA isolates. Panton-valentine leucocidin (PVL) genes were also detected by PCR. Among 273 S. aureus isolates, hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA), community-associated methicillin-resistant S. aureus (CA-MRSA), hospital-acquired methicillin susceptible Staphylococcus aureus (HA-MSSA) and community-associated methicillin susceptible Staphylococcus aureus (CA-MSSA) isolates accounted for 55.6, 1.5, 36.3, and 6.6%, respectively. Nine isolates were confirmed as hVISA by MET. Among 60 HA-MRSA isolates, ST239-MRSA-III was the most prevalent clone accounting for 51.7%, followed by ST5-MRSA-II clone. Fifty percentage and 22.2% of CA-MSSA isolates were found to be ST121 and ST88. ST239-MRSA-III was the predominant clone in hVISA isolates. However, no predominant ST type was found in HA-MSSA isolates. Of 9 PVL-positive strains, ST88 was the most prevalent ST (50.0%; 4/8), followed by ST121 (33.3%; 3/9), ST5 (4.5%; 1/22) and ST239 (2.6%; 1/39). In conclusion, ST239-III was the major pandemic clone in hVISA and HA-MRSA and spread in China. ST5-emerging rapidly in China had remained stable viability.

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Molecular characterization of Staphylococcus aureus isolates causing skin and soft tissue infections (SSTIs)

Cited by 55 publications

References 25 publications

Link between Genotype and Antimicrobial Resistance in Bovine Mastitis-Related Staphylococcus aureus Strains, Determined by Comparing Swiss and French Isolates from the Rhône Valley

Link between Genotype and Antimicrobial Resistance in Bovine Mastitis-Related Staphylococcus aureus Strains, Determined by Comparing Swiss and French Isolates from the Rhône Valley

Characterization of Staphylococcus aureus Strains Associated with Food Poisoning in Shenzhen, China

Molecular characteristic of Staphylococcus aureus isolates in a Chinese teaching hospital

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