Molecular characterization of soft-rot disease pathogen from Phalaenopsis and differences in genotype response to its infection

Sanjaya, I Putu Wahyu; Sudarsono, Sudarsono; Chan, Ming‐Tsair; Sukma, Dewi

doi:10.17660/actahortic.2022.1334.14

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…SRD symptoms were similar to rot symptoms on naturally infected leaves (positive method of Koch's postulates). This selected strain was validated using the 16S rRNA marker gene to detect the pathogen, and the sequencing data confirmed the bacteria was Dickeya dadantii (Sanjaya et al, 2022). Therefore, this pathogen is confirmed to be associated with soft rot disease in P. amabilis.…”

Section: Effects Of Pathogen Concentration On the Detached Leaves Of ...mentioning

confidence: 77%

Inoculation Methods to Determine Resistance of Phalaenopsis amabilis (L.) Regeneratded from Irradiated Protocorms to Dickeya dadantii

Putri,

Sukma,

Sudarsono

et al. 2024

Planta Tropika

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Soft-rot disease (SRD) in Phalaenopsis, caused Dickeya dadantii, has resulted in significant losses in the orchid sector in Indonesia. This study aimed to evaluate the inoculation method of Dickeya dadantii and identify the resistance response of individual regenerated plantlets of Phal. amabilis from irradiated protocorm. A detached leaf assay was used to evaluate the inoculation method and resistance response of SRD. Based on the results of this study, Dickeya dadantii bacteria could only infect the leaves through wounding tissue. The density of bacteria that could infect leaf tissue was OD600 = 0.2. All dilution factors tested caused soft rot symptoms in P. amabilis. On the other hand, Vanilla planifolia only showed symptoms at a dilution factor of 10-0. Four accessions of regenerated plantlets from irradiated protocorms were resistant to SRD. They were from irradiation 5 Gy (IP 05 Gy-23, IP 05 Gy-31, and IP 05 Gy-33) and one accession from the control treatment or without irradiation (IP 0 Gy -1). These results showed that 5 Gy irradiation increased plant resistance to SRD in Phalaenopsis. A dose of 5 Gy can potentially produce mutant lines resistant to SRD in Phalaenopsis or other plants, too.

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Section: Effects Of Pathogen Concentration On the Detached Leaves Of ...mentioning

confidence: 77%

Inoculation Methods to Determine Resistance of Phalaenopsis amabilis (L.) Regeneratded from Irradiated Protocorms to Dickeya dadantii

Putri,

Sukma,

Sudarsono

et al. 2024

Planta Tropika

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“…The latest study found that the Phalaenopsis leaf extract has antimicrobial potential for plant and human pathogens. [ 23 24 ] The possibility of obtaining effective antimicrobial compounds is increased using the pathogen-resistant Phalaenopsis species. Further studies to determine the bioactive compounds with antimicrobial activity from the two species can be explored to obtain detailed information on which compounds can be utilized for the potential use in biopharmaceuticals as antibiotics or in agriculture as alternative bio-bactericides.…”

Section: R Esults and Discussionmentioning

confidence: 99%

D-optimal Design Optimization of Solvent Mixture for Flavonoid Extraction from Phalaenopsis Leaves with Antioxidant Activity

Sanjaya,

Sudarsono,

Chan

et al. 2024

Journal of Pharmacy and Bioallied Sciences

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Aims: This study aimed to optimize the extraction of flavonoids and antioxidants from Phalaenopsis leaves by using solvent mixtures. Method: The total flavonoid content (TFC) and antioxidant activity were evaluated using the colorimetric method and ferric-reducing antioxidant power (FRAP), respectively. Maceration extracts from fresh leaves were used for the analysis. The study used the Design Expert 13.0 program to optimize the solvents (water, acetone, and methanol) and their combined ratio. Result: The results showed that 100% acetone was the best solvent for both responses, with a desirability value of 0.884, TFC of 0.434 mg QE/g fresh weight (FW) and FRAP of 713.53 μmol TE/g FW. Screening of the most potent Phalaenopsis genotypes for obtaining the most active leaf extract showed that P. amboinensis and P. pantherina were the best genotypes for TFC (0.786–0.797 mg QE/g FW) and FRAP activity (862.25–891.48 μmol TE/g FW). Conclusion: This study demonstrates an easy and useful way to obtain flavonoids and antioxidants from Phalaenopsis materials that can be used in the flower-based industry to make new functional ingredients.

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“…The seeds were extracted from seedpod resulting from selfpollinated wild D. crumenatum obtained in Bogor, West Java. The extracted seeds then cultured in in vitro culture following Sanjaya et al (2022) germination medium. Briefly half-strength Murashige and Skoog medium supplemented with 15% coconut water, 30 g L -1 sugar, 7 g L -1 agar.…”

Section: Methodsmentioning

confidence: 99%

Intraspecific SSR Marker Screening for Detection of Dendrobium crumenatum Mutants Generated from In Vitro Gamma Irradiation

Dwiyani,

Sanjaya,

Yuswanti

et al. 2024

J.Tropical Biodiversity Biotechnology

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Determination of D. crumenatum mutant obtained from in vitro mutation breeding needs a long time due to its long-life cycle. SSR molecular markers can be used for early mutant detection. Specific SSR markers developed in D. crumenatum are not yet available. Alternative published SSR markers were developed from D. catenatum. The aims of this study are to screen the most informative SSR markers generated from D. catenatum tested in irradiated D. crumenatum population and to determine the gamma irradiation dose resulting the most mutants. Ten SSR markers were randomly selected and tested in 25 individuals of D. crumenatum plantlets irradiated with several doses (0, 5, 10, 15, and 20 Gy; 5 plantlets each dose). The result showed 7 of 10 primers were polymorphic and the other three were monomorphic. All seven polymorphic primers can be used to identified intraspecific variation in the D. crumenatum mutant population. Markers dnsr28 and dnsr98 were the most informative, with the highest polymorphic information content (PIC) value of 0.5. Irradiation D. crumenatum protocorms using 10-15 gray doses were detected as the highest mutant percentage obtained up to 100% in the sample tested. This resulting marker information can be used to screen wider mutant population to decrease the non-mutant individuals in the population for maintenance and cost efficiency. The 10-15 Gy can be used as reference doses for gamma irradiation in 3 months old D. crumenatum protocorm materials.

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Molecular characterization of soft-rot disease pathogen from Phalaenopsis and differences in genotype response to its infection

Cited by 3 publications

References 21 publications

Inoculation Methods to Determine Resistance of Phalaenopsis amabilis (L.) Regeneratded from Irradiated Protocorms to Dickeya dadantii

Inoculation Methods to Determine Resistance of Phalaenopsis amabilis (L.) Regeneratded from Irradiated Protocorms to Dickeya dadantii

D-optimal Design Optimization of Solvent Mixture for Flavonoid Extraction from Phalaenopsis Leaves with Antioxidant Activity

Intraspecific SSR Marker Screening for Detection of <i>Dendrobium crumenatum</i> Mutants Generated from <i>In Vitro</i> Gamma Irradiation

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