Molecular Characterization of Segment 6 of Bluetongue Serotype 16 of Sheep Origin from India

Ranjan, Koushlesh; Prasad, Gaya; Kumar, Pawan; Prasad, Minakshi

doi:10.14737/journal.aavs/2014/2.2.98.103

Cited by 3 publications

(6 citation statements)

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“…Confirmation of serotype has been done by sequencing of the cDNA amplicons and subsequently phylogenetic analysis is done for comparing with reference strains that are previously characterized [52,54]. The RT-PCR assay provides a rapid as well as sensitive and reliable method to identify and differentiate all the serotypes of BTV [45,50,51,[55][56][57][58].…”

Section: 2mentioning

confidence: 99%

“…The virus isolation is performed in embryonated chicken eggs, in cell culture (BHK-21 or Vero cell line), or occasionally in sheep [ 139 ]. The virus is serotyped either by virus neutralization tests such as plaque reduction, plaque inhibition, Microtiter neutralization, and Fluorescence inhibition test (FIT) or through reverse-transcription polymerase chain reaction (RT-PCR) (a prescribed test for international trade) [ 50 , 51 ]. A highly sensitive silver staining method of RNA-polyacrylamide gel electrophoresis (RNA-PAGE) of bluetongue virus was developed recently [ 49 ].…”

Section: Advances In Diagnosis Of Respiratory Diseases Of Small Rumentioning

confidence: 99%

“…Analyses of the nucleotide sequence as well as phylogenetic comparisons of genome segment 2 that encodes outer-capsid protein VP2 help in creation of segment-2 database [ 53 ]. Such database is used for developing rapid as well as reliable typing assay based on RT-PCR [ 50 , 51 , 54 ]. Testing of multiple primer pairs has also been done that provides an identification of serotype initially by amplifying a cDNA product of the expected size.…”

Section: Advances In Diagnosis Of Respiratory Diseases Of Small Rumentioning

confidence: 99%

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Advances in Diagnosis of Respiratory Diseases of Small Ruminants

Chakraborty¹,

Kumar

Tiwari

et al. 2014

Veterinary Medicine International

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Irrespective of aetiology, infectious respiratory diseases of sheep and goats contribute to 5.6 percent of the total diseases of small ruminants. These infectious respiratory disorders are divided into two groups: the diseases of upper respiratory tract, namely, nasal myiasis and enzootic nasal tumors, and diseases of lower respiratory tract, namely, peste des petits ruminants (PPR), parainfluenza, Pasteurellosis, Ovine progressive pneumonia, mycoplasmosis, caprine arthritis encephalitis virus, caseous lymphadenitis, verminous pneumonia, and many others. Depending upon aetiology, many of them are acute and fatal in nature. Early, rapid, and specific diagnosis of such diseases holds great importance to reduce the losses. The advanced enzyme-linked immunosorbent assays (ELISAs) for the detection of antigen as well as antibodies directly from the samples and molecular diagnostic assays along with microsatellites comprehensively assist in diagnosis as well as treatment and epidemiological studies. The present review discusses the advancements made in the diagnosis of common infectious respiratory diseases of sheep and goats. It would update the knowledge and help in adapting and implementing appropriate, timely, and confirmatory diagnostic procedures. Moreover, it would assist in designing appropriate prevention protocols and devising suitable control strategies to overcome respiratory diseases and alleviate the economic losses.

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Section: 2mentioning

confidence: 99%

Section: Advances In Diagnosis Of Respiratory Diseases Of Small Rumentioning

confidence: 99%

Section: Advances In Diagnosis Of Respiratory Diseases Of Small Rumentioning

confidence: 99%

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Advances in Diagnosis of Respiratory Diseases of Small Ruminants

Chakraborty¹,

Kumar

Tiwari

et al. 2014

Veterinary Medicine International

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“…Variation in the outer capsid protein genes ( VP2 and VP5 ) to different extents is reported among the isolates of a particular serotype in different topotypes [ 9 ]. A high percentage of sequence identity was also observed between Indian, and most of the other global BTV-16 isolates except NIG/AJ586694 reported from Nigeria [ 19 ]. A new BTV 16 isolate from Andhra Pradesh was found to be very close to other Indian BTV 16 isolates at the nucleotide sequence identity of segment 6 [ 20 ].…”

Section: Discussionmentioning

confidence: 89%

Genetic and phylogenetic analysis of the outer capsid protein genes of Indian isolates of bluetongue virus serotype-16

et al. 2018

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Aim:The aim of the study was to characterize bluetongue virus serotype 16 (BTV-16), recently isolated from different states of India. The evolutionary relationship of newly isolated BTV-16 and previously reported Indian and global BTV-16 isolates were compared using molecular analysis.Materials and Methods:In the present study, five (n=5) BTV-16 isolates were used to amplify gene segment-2 and segment-6 encoding the outer capsid proteins VP2 and VP5, respectively. The amplified products were purified and sequenced by the Sanger sequencing method. The phylogenetic relationship and nucleotide identity of all five BTV-16 isolates were compared with previously reported Indian and global BTV-16 isolates. Nucleotide sequence data were aligned using the CLUSTAL W algorithm implemented in the MegAlign of DNASTAR program package (MegAlign 5.00, DNASTAR Inc., Madison, USA). Phylogenetic analyses were carried out using MEGA version 6.0 software with the best nucleotide substitution model.Results:Phylogenetic analysis based on the VP2 and VP5 encoding genes, segregates Indian BTV-16 isolates in a distinct cluster with proximity to the Eastern topotype. Indian isolates make a monophyletic cluster with Eastern topotypes with Western topotype BTV-16 (BTV-16/NIG/AJ586694) occupying a separate cluster. Indian isolates were found to share 91.5%-97.5% and 96.5%-98.9% identity at the nucleotide and deduced amino acid (aa) level, respectively, to the global BTV-16 isolates. There is a high degree of variation with the Nigerian isolate with 27.0-27.7% and 26.0-26.9% at the nucleotide and aa sequence level, respectively. These data suggest that Indian BTV-16 isolates might have evolved separately within the Eastern BTV topotype.Conclusion:Phylogenetic analyses and nucleotide identity of BTV-16 isolates at the VP2 and VP5 gene encoded level indicate that isolates used in the present study might have evolved from a common Eastern topotype ancestor. The data presented in this study will be helpful for future selection of reference strains in a serological and molecular epidemiology study.

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“…Th e segment-6-based analyses of Indian BTVs also showed the eastern and western topotype origin. Segment 6 of Indian isolates of BTV2 (BT2) and BTV9 (Ong5/06/Ind) showed western origin (Ranjan et al, 2013(Ranjan et al, , 2014a, whereas BTV16 (BT16/07) and BTV1 showed eastern origin (Ranjan et al, 2014b;Manjunatha et al, 2010). Moreover, Indian viruses BTV1 (IND1988/0 1), BTV3 (IND2003/08), BTV9 (IND2005/01), BTV16 (IND/ Goat/2010/16/HSR), BTV21 (Kmno-7/06/IND) and BTV23 (IND1988/02) were grouped as eastern topotype with >77.1% identity with previously characterized homologous serotypes of eastern origin (Maan et al, 2015).…”

Section: Segmentmentioning

confidence: 99%

Bluetongue: Indian perspective

Ranjan¹,

Minakshi²,

Prasad³

2015

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Bluetongue (BT) is an insect borne (Culicoides) viral disease of small ruminants in India. While seroprevalence for BT is observed mostly in domestic and wild ruminant animals, the clinical form of disease and severe mortality is observed in sheep. Since the fi rst report of BT in 1960s the country became endemic for the disease and most of the BT virus (BTV) serotypes (22 out of 27 worldwide) have been reported. Th e genome sequence analyses of these viruses revealed that both the eastern and western topotypes as well as their reassortant strains are present in India. It further revealed that some of these viruses are very close to live vaccines used in other countries. Th e severe economic concern justifi es the need to develop sensitive and reliable diagnostic tests for BT. Th e virus isolation followed by identifi cation by electron microscopy is gold standard test, but it is time consuming and not easily available in all the laboratories. Th erefore, nucleic acid-based rapid diagnostic tests such as PCR, real-time PCR etc. are used nowadays. Th e BT control program in India includes vector control as well as eff ective vaccination. Th e vector population is controlled by vector traps, synthetic pesticides and some of the herbal compounds. For eff ective vaccination, the serotypes prevalent in a particular geographical area must be known, which can be achieved by continuous monitoring and sero-surveillance of disease. Th e multivalent inactivated vaccines are more suitable for India in comparison to modifi ed live vaccines as the latter may turn to virulent and may lead to severe outbreak of the disease.

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Molecular Characterization of Segment 6 of Bluetongue Serotype 16 of Sheep Origin from India

Cited by 3 publications

References 18 publications

Advances in Diagnosis of Respiratory Diseases of Small Ruminants

Advances in Diagnosis of Respiratory Diseases of Small Ruminants

Genetic and phylogenetic analysis of the outer capsid protein genes of Indian isolates of bluetongue virus serotype-16

Bluetongue: Indian perspective

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