Molecular Characterization of Rice OsLCB2a1 Gene and Functional Analysis of its Role in Insect Resistance

Begum, Mahfuj Ara; Shi, Xiaoxiao; Tan, Ye; Zhou, Wenwu; Hannun, Yusuf A.; Obeid, Lina M.; Mao, Cungui; Zhu, Zhiliang

doi:10.3389/fpls.2016.01789

Cited by 14 publications

(7 citation statements)

References 68 publications

(83 reference statements)

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“…Overexpression of OsLCB2a in Arabidopsis led to the accumulation of LCB and ceramides compared with WT. These transgenic plants also displayed increased callose and wax deposition, an induction of SA-dependent and camalexin-dependent genes but a reduction of JA-related genes, and inhibited aphid infestation (Begum et al, 2016).…”

Section: Signalling Messengers In Biotic Stressmentioning

confidence: 93%

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Sphingolipids: towards an integrated view of metabolism during the plant stress response

et al. 2019

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Plants exist in an environment of changing abiotic and biotic stresses. They have developed a complex set of strategies to respond to these stresses and over recent years it has become clear that sphingolipids are a key player in these responses. Sphingolipids are not universally present in all three domains of life. Many bacteria and archaea do not produce sphingolipids but they are ubiquitous in eukaryotes and have been intensively studied in yeast and mammals. During the last decade there has been a steadily increasing interest in plant sphingolipids. Plant sphingolipids exhibit structural differences when compared with their mammalian counterparts and it is now clear that they perform some unique functions. Sphingolipids are recognised as critical components of the plant plasma membrane and endomembrane system. Besides being important structural elements of plant membranes, their particular structure contributes to the fluidity and biophysical order. Sphingolipids are also involved in multiple cellular and regulatory processes including vesicle trafficking, plant development and defence. This review will focus on our current knowledge as to the function of sphingolipids during plant stress responses, not only as structural components of biological membranes, but also as signalling mediators.

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Section: Signalling Messengers In Biotic Stressmentioning

confidence: 93%

“…The role of sphingolipid metabolism in response to herbivory has been analysed (Begum et al, 2016). Overexpression of OsLCB2a in Arabidopsis led to the accumulation of LCB and ceramides compared with WT.…”

Section: Signalling Messengers In Biotic Stressmentioning

confidence: 99%

Sphingolipids: towards an integrated view of metabolism during the plant stress response

et al. 2019

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“…However, the fatty acid hydroxylase we identified does not appear to have high similarity to CER1 from cabbage or Arabidopsis. Regarding the palmitoyltransferase, overexpression of a rice gene, OsLCB2a1 , which encodes a subunit of a serine palmitoyltransferase, an enzyme important for sphingolipid biosynthesis, has been shown to result in higher wax deposition on leaves of transgenic Arabidopsis plants ( Begum et al, 2016 ). From our scans of the diploid genome, a gene (CUFF.9282.1) in the color QTL region on LG02 was found, which was annotated as very-long-chain 3-ketoacyl-CoA synthase (KCS) on the Genome Database for Vaccinium (GDV) website 3 .…”

Section: Discussionmentioning

confidence: 99%

High-Density Linkage Map Construction and QTL Identification in a Diploid Blueberry Mapping Population

Ogden

Bostan

et al. 2021

Front. Plant Sci.

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Genotyping by sequencing approaches have been widely applied in major crops and are now being used in horticultural crops like berries and fruit trees. As the original and largest producer of cultivated blueberry, the United States maintains the most diverse blueberry germplasm resources comprised of many species of different ploidy levels. We previously constructed an interspecific mapping population of diploid blueberry by crossing the parent F1#10 (Vaccinium darrowii Fla4B × diploid V. corymbosum W85–20) with the parent W85–23 (diploid V. corymbosum). Employing the Capture-Seq technology developed by RAPiD Genomics, with an emphasis on probes designed in predicted gene regions, 117 F1 progeny, the two parents, and two grandparents of this population were sequenced, yielding 131.7 Gbp clean sequenced reads. A total of 160,535 single nucleotide polymorphisms (SNPs), referenced to 4,522 blueberry genome sequence scaffolds, were identified and subjected to a parent-dependent sliding window approach to further genotype the population. Recombination breakpoints were determined and marker bins were deduced to construct a high density linkage map. Twelve blueberry linkage groups (LGs) consisting of 17,486 SNP markers were obtained, spanning a total genetic distance of 1,539.4 cM. Among 18 horticultural traits phenotyped in this population, quantitative trait loci (QTLs) that were significant over at least 2 years were identified for chilling requirement, cold hardiness, and fruit quality traits of color, scar size, and firmness. Interestingly, in 1 year, a QTL associated with timing of early bloom, full bloom, petal fall, and early green fruit was identified in the same region harboring the major QTL for chilling requirement. In summary, we report here the first high density bin map of a diploid blueberry mapping population and the identification of several horticulturally important QTLs.

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“…To date, the role of sphingolipids in the biotic stress response to herbivores is undefined. Recently, Begum et al (2016) reported that the OsLCB2a1 gene encoding a subunit of serine palmitoyltransferase (SPT) plays a critical role in the plant defense response to brown planthopper (BPH) attack and protected plants from herbivore infestation upon upregulation. Gene expression analysis in rice seedlings revealed the enhanced transcript levels of OsLCB2a1 gene at 4 h but decreased at 8-24 h after BPH attack.…”

Section: Sphingolipids In Plant-herbivore Interactionsmentioning

confidence: 99%

“…Sphingolipidomic analysis revealed elevated levels of trihydroxylated LCB, phytosphingosine, and phytoceramide by 1.7-and 1.3-fold, respectively. In addition, the reduced population size of the green peach aphid (Myzus persicae) was observed in transgenic Arabidopsis overexpressing OsLCB2a1 gene (Begum et al, 2016). Further investigation is needed to determine which signals were generated due to the overexpression of OsLCB2a1 gene in rice that protected the plants from brown planthopper infestation.…”

Section: Sphingolipids In Plant-herbivore Interactionsmentioning

confidence: 99%

Emerging Roles of Sphingolipid Signaling in Plant Response to Biotic and Abiotic Stresses

et al. 2018

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Plant sphingolipids are not only structural components of the plasma membrane and other endomembrane systems but also act as signaling molecules during biotic and abiotic stresses. However, the roles of sphingolipids in plant signal transduction in response to environmental cues are yet to be investigated in detail. In this review, we discuss the signaling roles of sphingolipid metabolites with a focus on plant sphingolipids. We also mention some microbial sphingolipids that initiate signals during their interaction with plants, because of the limited literatures on their plant analogs. The equilibrium of nonphosphorylated and phosphorylated sphingolipid species determine the destiny of plant cells, whereas molecular connections among the enzymes responsible for this equilibrium in a coordinated signaling network are poorly understood. A mechanistic link between the phytohormone-sphingolipid interplay has also not yet been fully understood and many key participants involved in this complex interaction operating under stress conditions await to be identified. Future research is needed to fill these gaps and to better understand the signal pathways of plant sphingolipids and their interplay with other signals in response to environmental stresses.

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Molecular Characterization of Rice OsLCB2a1 Gene and Functional Analysis of its Role in Insect Resistance

Cited by 14 publications

References 68 publications

Sphingolipids: towards an integrated view of metabolism during the plant stress response

Sphingolipids: towards an integrated view of metabolism during the plant stress response

High-Density Linkage Map Construction and QTL Identification in a Diploid Blueberry Mapping Population

Emerging Roles of Sphingolipid Signaling in Plant Response to Biotic and Abiotic Stresses

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